Cargando…

Molecular characterizations of Giardia duodenalis based on multilocus genotyping in sheep, goats, and beef cattle in Southwest Inner Mongolia, China

Giardia duodenalis is an important zoonotic parasite that causes economic losses to animal husbandry and threatens public health. In the present study, a total of 1466 fresh fecal samples were collected from sheep (n = 797), goats (n = 561) and beef cattle (n = 108) in Southwest Inner Mongolia, Chin...

Descripción completa

Detalles Bibliográficos
Autores principales: Fu, Yin, Dong, Heping, Bian, Xiaokun, Qin, Ziyang, Han, Han, Lang, Jiashu, Zhang, Junchen, Zhao, Guanghui, Li, Junqiang, Zhang, Longxian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9265451/
https://www.ncbi.nlm.nih.gov/pubmed/35801842
http://dx.doi.org/10.1051/parasite/2022036
_version_ 1784743217397235712
author Fu, Yin
Dong, Heping
Bian, Xiaokun
Qin, Ziyang
Han, Han
Lang, Jiashu
Zhang, Junchen
Zhao, Guanghui
Li, Junqiang
Zhang, Longxian
author_facet Fu, Yin
Dong, Heping
Bian, Xiaokun
Qin, Ziyang
Han, Han
Lang, Jiashu
Zhang, Junchen
Zhao, Guanghui
Li, Junqiang
Zhang, Longxian
author_sort Fu, Yin
collection PubMed
description Giardia duodenalis is an important zoonotic parasite that causes economic losses to animal husbandry and threatens public health. In the present study, a total of 1466 fresh fecal samples were collected from sheep (n = 797), goats (n = 561) and beef cattle (n = 108) in Southwest Inner Mongolia, China. Giardia duodenalis was initially screened via nested polymerase chain reaction (PCR) targeting the β-giardin (bg) gene, and bg-positive samples were subjected to PCR amplification targeting the glutamate dehydrogenase (gdh) and triose phosphate isomerase (tpi) genes. A total of 4.0% of samples (58/1466) were positive for G. duodenalis, with a prevalence of 3.4% in sheep, 3.7% in goats and 5.2% in beef cattle. Three G. duodenalis assemblages (A, B, and E) were identified, with E as the prevalent assemblage. Four and one novel assemblage E sequences were obtained for the gdh and tpi loci, respectively and four assemblage E multilocus genotypes (MLG) were obtained. This study demonstrates high genetic variations in G. duodenalis assemblage E, and provides baseline data for preventing and controlling G. duodenalis infection in livestock in Inner Mongolia.
format Online
Article
Text
id pubmed-9265451
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher EDP Sciences
record_format MEDLINE/PubMed
spelling pubmed-92654512022-07-27 Molecular characterizations of Giardia duodenalis based on multilocus genotyping in sheep, goats, and beef cattle in Southwest Inner Mongolia, China Fu, Yin Dong, Heping Bian, Xiaokun Qin, Ziyang Han, Han Lang, Jiashu Zhang, Junchen Zhao, Guanghui Li, Junqiang Zhang, Longxian Parasite Research Article Giardia duodenalis is an important zoonotic parasite that causes economic losses to animal husbandry and threatens public health. In the present study, a total of 1466 fresh fecal samples were collected from sheep (n = 797), goats (n = 561) and beef cattle (n = 108) in Southwest Inner Mongolia, China. Giardia duodenalis was initially screened via nested polymerase chain reaction (PCR) targeting the β-giardin (bg) gene, and bg-positive samples were subjected to PCR amplification targeting the glutamate dehydrogenase (gdh) and triose phosphate isomerase (tpi) genes. A total of 4.0% of samples (58/1466) were positive for G. duodenalis, with a prevalence of 3.4% in sheep, 3.7% in goats and 5.2% in beef cattle. Three G. duodenalis assemblages (A, B, and E) were identified, with E as the prevalent assemblage. Four and one novel assemblage E sequences were obtained for the gdh and tpi loci, respectively and four assemblage E multilocus genotypes (MLG) were obtained. This study demonstrates high genetic variations in G. duodenalis assemblage E, and provides baseline data for preventing and controlling G. duodenalis infection in livestock in Inner Mongolia. EDP Sciences 2022-07-06 /pmc/articles/PMC9265451/ /pubmed/35801842 http://dx.doi.org/10.1051/parasite/2022036 Text en © Y. Fu et al., published by EDP Sciences, 2022 https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Fu, Yin
Dong, Heping
Bian, Xiaokun
Qin, Ziyang
Han, Han
Lang, Jiashu
Zhang, Junchen
Zhao, Guanghui
Li, Junqiang
Zhang, Longxian
Molecular characterizations of Giardia duodenalis based on multilocus genotyping in sheep, goats, and beef cattle in Southwest Inner Mongolia, China
title Molecular characterizations of Giardia duodenalis based on multilocus genotyping in sheep, goats, and beef cattle in Southwest Inner Mongolia, China
title_full Molecular characterizations of Giardia duodenalis based on multilocus genotyping in sheep, goats, and beef cattle in Southwest Inner Mongolia, China
title_fullStr Molecular characterizations of Giardia duodenalis based on multilocus genotyping in sheep, goats, and beef cattle in Southwest Inner Mongolia, China
title_full_unstemmed Molecular characterizations of Giardia duodenalis based on multilocus genotyping in sheep, goats, and beef cattle in Southwest Inner Mongolia, China
title_short Molecular characterizations of Giardia duodenalis based on multilocus genotyping in sheep, goats, and beef cattle in Southwest Inner Mongolia, China
title_sort molecular characterizations of giardia duodenalis based on multilocus genotyping in sheep, goats, and beef cattle in southwest inner mongolia, china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9265451/
https://www.ncbi.nlm.nih.gov/pubmed/35801842
http://dx.doi.org/10.1051/parasite/2022036
work_keys_str_mv AT fuyin molecularcharacterizationsofgiardiaduodenalisbasedonmultilocusgenotypinginsheepgoatsandbeefcattleinsouthwestinnermongoliachina
AT dongheping molecularcharacterizationsofgiardiaduodenalisbasedonmultilocusgenotypinginsheepgoatsandbeefcattleinsouthwestinnermongoliachina
AT bianxiaokun molecularcharacterizationsofgiardiaduodenalisbasedonmultilocusgenotypinginsheepgoatsandbeefcattleinsouthwestinnermongoliachina
AT qinziyang molecularcharacterizationsofgiardiaduodenalisbasedonmultilocusgenotypinginsheepgoatsandbeefcattleinsouthwestinnermongoliachina
AT hanhan molecularcharacterizationsofgiardiaduodenalisbasedonmultilocusgenotypinginsheepgoatsandbeefcattleinsouthwestinnermongoliachina
AT langjiashu molecularcharacterizationsofgiardiaduodenalisbasedonmultilocusgenotypinginsheepgoatsandbeefcattleinsouthwestinnermongoliachina
AT zhangjunchen molecularcharacterizationsofgiardiaduodenalisbasedonmultilocusgenotypinginsheepgoatsandbeefcattleinsouthwestinnermongoliachina
AT zhaoguanghui molecularcharacterizationsofgiardiaduodenalisbasedonmultilocusgenotypinginsheepgoatsandbeefcattleinsouthwestinnermongoliachina
AT lijunqiang molecularcharacterizationsofgiardiaduodenalisbasedonmultilocusgenotypinginsheepgoatsandbeefcattleinsouthwestinnermongoliachina
AT zhanglongxian molecularcharacterizationsofgiardiaduodenalisbasedonmultilocusgenotypinginsheepgoatsandbeefcattleinsouthwestinnermongoliachina