Halomonas salinarum sp. nov., a moderately halophilic bacterium isolated from saline soil in Yingkou, China

Strain G5-11(T), a Gram-negative, moderately halotolerant, facultatively aerobic, motile bacterium was isolated from saline soil collected from Yingkou, Liaoning, China. The cells of strain G5-11(T) grew in the presence of 3–15% (w/v) NaCl (optimum 5%), at between 4 and 35 °C (optimum 30 °C), and at a pH of 6.0–9.0 (optimum 8.0). The major respiratory quinone was Q-9 and the dominant cellular fatty acids were summed feature 8 (C(18:1)ω7c/C(18:1)ω6c), C(16:0), and summed feature 3 (C(16:1)ω7c/C(16:1)ω6c). The major components of the polar lipid profile were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and unidentified aminolipid. The G + C content of the strain G5-11(T) genome was 61.0 mol%. The isolated strain G5-11(T) showed the highest 16S rRNA gene similarity to Halomonas niordiana LMG 31227(T) and Halomonas taeanensis DSM 16463(T), both reaching 98.3%, followed by Halomonas pacifica NBRC 102220(T). The results from phenotypic, chemotaxonomic, and phylogenetic analyses showed that strain G5-11(T) represented a novel species of the genus Halomonas, for which the name Halomonas salinarum sp. nov. was proposed. The type strain of Halomonas salinarum is G5-11(T) (= CGMCC 1.12051(T) = LMG 31677(T)). SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00203-022-03032-3.