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Microplastics Affect the Inflammation Pathway in Human Gingival Fibroblasts: A Study in the Adriatic Sea

The level of environmental microplastics in the sea is constantly increasing. They can enter the human body with food, be absorbed through the gut and have negative effects on the organism’s health after its digestion. To date, microplastics (MPs) are considered new environmental pollutants in the a...

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Autores principales: Caputi, Sergio, Diomede, Francesca, Lanuti, Paola, Marconi, Guya Diletta, Di Carlo, Piero, Sinjari, Bruna, Trubiani, Oriana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266176/
https://www.ncbi.nlm.nih.gov/pubmed/35805437
http://dx.doi.org/10.3390/ijerph19137782
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author Caputi, Sergio
Diomede, Francesca
Lanuti, Paola
Marconi, Guya Diletta
Di Carlo, Piero
Sinjari, Bruna
Trubiani, Oriana
author_facet Caputi, Sergio
Diomede, Francesca
Lanuti, Paola
Marconi, Guya Diletta
Di Carlo, Piero
Sinjari, Bruna
Trubiani, Oriana
author_sort Caputi, Sergio
collection PubMed
description The level of environmental microplastics in the sea is constantly increasing. They can enter the human body with food, be absorbed through the gut and have negative effects on the organism’s health after its digestion. To date, microplastics (MPs) are considered new environmental pollutants in the air sea and they are attracting wide attention. The possible toxic effects of MPs isolated at different sea depths of 1, 24 and 78 m were explored in an in vitro model of human gingival fibroblasts (hGFs). MPs isolated from the sea showed different size and were then divided into different sample groups: 1, 24 and 78 m. The results obtained revealed that MPs are able to activate the inflammatory pathway NFkB/MyD88/NLRP3. In detail, the exposure to MPs from 1 and 78 m led to increased levels of inflammatory markers NFkB, MyD88 and NLRP3 in terms of proteins and gene expression. Moreover, cells exposed to MPs showed a lower metabolic activity rate compared to unexposed cells. In conclusion, these findings demonstrate that the inflammation process is stimulated by MPs exposure, providing a new perspective to better understand the intracellular mechanism.
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spelling pubmed-92661762022-07-09 Microplastics Affect the Inflammation Pathway in Human Gingival Fibroblasts: A Study in the Adriatic Sea Caputi, Sergio Diomede, Francesca Lanuti, Paola Marconi, Guya Diletta Di Carlo, Piero Sinjari, Bruna Trubiani, Oriana Int J Environ Res Public Health Article The level of environmental microplastics in the sea is constantly increasing. They can enter the human body with food, be absorbed through the gut and have negative effects on the organism’s health after its digestion. To date, microplastics (MPs) are considered new environmental pollutants in the air sea and they are attracting wide attention. The possible toxic effects of MPs isolated at different sea depths of 1, 24 and 78 m were explored in an in vitro model of human gingival fibroblasts (hGFs). MPs isolated from the sea showed different size and were then divided into different sample groups: 1, 24 and 78 m. The results obtained revealed that MPs are able to activate the inflammatory pathway NFkB/MyD88/NLRP3. In detail, the exposure to MPs from 1 and 78 m led to increased levels of inflammatory markers NFkB, MyD88 and NLRP3 in terms of proteins and gene expression. Moreover, cells exposed to MPs showed a lower metabolic activity rate compared to unexposed cells. In conclusion, these findings demonstrate that the inflammation process is stimulated by MPs exposure, providing a new perspective to better understand the intracellular mechanism. MDPI 2022-06-24 /pmc/articles/PMC9266176/ /pubmed/35805437 http://dx.doi.org/10.3390/ijerph19137782 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Caputi, Sergio
Diomede, Francesca
Lanuti, Paola
Marconi, Guya Diletta
Di Carlo, Piero
Sinjari, Bruna
Trubiani, Oriana
Microplastics Affect the Inflammation Pathway in Human Gingival Fibroblasts: A Study in the Adriatic Sea
title Microplastics Affect the Inflammation Pathway in Human Gingival Fibroblasts: A Study in the Adriatic Sea
title_full Microplastics Affect the Inflammation Pathway in Human Gingival Fibroblasts: A Study in the Adriatic Sea
title_fullStr Microplastics Affect the Inflammation Pathway in Human Gingival Fibroblasts: A Study in the Adriatic Sea
title_full_unstemmed Microplastics Affect the Inflammation Pathway in Human Gingival Fibroblasts: A Study in the Adriatic Sea
title_short Microplastics Affect the Inflammation Pathway in Human Gingival Fibroblasts: A Study in the Adriatic Sea
title_sort microplastics affect the inflammation pathway in human gingival fibroblasts: a study in the adriatic sea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266176/
https://www.ncbi.nlm.nih.gov/pubmed/35805437
http://dx.doi.org/10.3390/ijerph19137782
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