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Specific Cerebrospinal Fluid SerpinA1 Isoform Pattern in Alzheimer’s Disease
SerpinA1 (α1-antitrypsin) is a soluble glycoprotein, the cerebrospinal fluid (CSF) isoforms of which showed disease-specific changes in neurodegenerative disorders that are still unexplored in Alz-heimer’s disease (AD). By means of capillary isoelectric focusing immunoassay, we investigated six serp...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266332/ https://www.ncbi.nlm.nih.gov/pubmed/35805926 http://dx.doi.org/10.3390/ijms23136922 |
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author | Barba, Lorenzo Halbgebauer, Steffen Paolini Paoletti, Federico Bellomo, Giovanni Abu-Rumeileh, Samir Steinacker, Petra Massa, Federico Parnetti, Lucilla Otto, Markus |
author_facet | Barba, Lorenzo Halbgebauer, Steffen Paolini Paoletti, Federico Bellomo, Giovanni Abu-Rumeileh, Samir Steinacker, Petra Massa, Federico Parnetti, Lucilla Otto, Markus |
author_sort | Barba, Lorenzo |
collection | PubMed |
description | SerpinA1 (α1-antitrypsin) is a soluble glycoprotein, the cerebrospinal fluid (CSF) isoforms of which showed disease-specific changes in neurodegenerative disorders that are still unexplored in Alz-heimer’s disease (AD). By means of capillary isoelectric focusing immunoassay, we investigated six serpinA1 isoforms in CSF samples of controls (n = 29), AD-MCI (n = 29), AD-dem (n = 26) and Lewy body disease (LBD, n = 59) patients and correlated the findings with CSF AD core biomarkers (Aβ42/40 ratio, p-tau, t-tau). Four CSF serpinA1 isoforms were differently expressed in AD patients compared to controls and LBD patients, especially isoforms 2 and 4. AD-specific changes were found since the MCI stage and significantly correlated with decreased Aβ42/40 (p < 0.05) and in-creased p-tau and t-tau levels in CSF (p < 0.001). Analysis of serpinA1 isoform provided good di-agnostic accuracy in discriminating AD patients versus controls (AUC = 0.80) and versus LBD patients (AUC = 0.92), with best results in patients in the dementia stage (AUC = 0.97). SerpinA1 isoform expression is altered in AD patients, suggesting a common, albeit disease-specific, in-volvement of serpinA1 in most neurodegenerative disorders. |
format | Online Article Text |
id | pubmed-9266332 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92663322022-07-09 Specific Cerebrospinal Fluid SerpinA1 Isoform Pattern in Alzheimer’s Disease Barba, Lorenzo Halbgebauer, Steffen Paolini Paoletti, Federico Bellomo, Giovanni Abu-Rumeileh, Samir Steinacker, Petra Massa, Federico Parnetti, Lucilla Otto, Markus Int J Mol Sci Article SerpinA1 (α1-antitrypsin) is a soluble glycoprotein, the cerebrospinal fluid (CSF) isoforms of which showed disease-specific changes in neurodegenerative disorders that are still unexplored in Alz-heimer’s disease (AD). By means of capillary isoelectric focusing immunoassay, we investigated six serpinA1 isoforms in CSF samples of controls (n = 29), AD-MCI (n = 29), AD-dem (n = 26) and Lewy body disease (LBD, n = 59) patients and correlated the findings with CSF AD core biomarkers (Aβ42/40 ratio, p-tau, t-tau). Four CSF serpinA1 isoforms were differently expressed in AD patients compared to controls and LBD patients, especially isoforms 2 and 4. AD-specific changes were found since the MCI stage and significantly correlated with decreased Aβ42/40 (p < 0.05) and in-creased p-tau and t-tau levels in CSF (p < 0.001). Analysis of serpinA1 isoform provided good di-agnostic accuracy in discriminating AD patients versus controls (AUC = 0.80) and versus LBD patients (AUC = 0.92), with best results in patients in the dementia stage (AUC = 0.97). SerpinA1 isoform expression is altered in AD patients, suggesting a common, albeit disease-specific, in-volvement of serpinA1 in most neurodegenerative disorders. MDPI 2022-06-22 /pmc/articles/PMC9266332/ /pubmed/35805926 http://dx.doi.org/10.3390/ijms23136922 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Barba, Lorenzo Halbgebauer, Steffen Paolini Paoletti, Federico Bellomo, Giovanni Abu-Rumeileh, Samir Steinacker, Petra Massa, Federico Parnetti, Lucilla Otto, Markus Specific Cerebrospinal Fluid SerpinA1 Isoform Pattern in Alzheimer’s Disease |
title | Specific Cerebrospinal Fluid SerpinA1 Isoform Pattern in Alzheimer’s Disease |
title_full | Specific Cerebrospinal Fluid SerpinA1 Isoform Pattern in Alzheimer’s Disease |
title_fullStr | Specific Cerebrospinal Fluid SerpinA1 Isoform Pattern in Alzheimer’s Disease |
title_full_unstemmed | Specific Cerebrospinal Fluid SerpinA1 Isoform Pattern in Alzheimer’s Disease |
title_short | Specific Cerebrospinal Fluid SerpinA1 Isoform Pattern in Alzheimer’s Disease |
title_sort | specific cerebrospinal fluid serpina1 isoform pattern in alzheimer’s disease |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266332/ https://www.ncbi.nlm.nih.gov/pubmed/35805926 http://dx.doi.org/10.3390/ijms23136922 |
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