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Loss of NSE-4 Perturbs Genome Stability and DNA Repair in Caenorhabditis elegans

The Structural Maintenance of Chromosomes (SMC) complex plays an important role in maintaining chromosome integrity, in which the SMC5/6 complex occupies a central position by facilitating mitotic and meiotic processes as well as DNA repair. NSE-4 Kleisin is critical for both the organization and fu...

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Autores principales: Odiba, Arome Solomon, Ezechukwu, Chiemekam Samuel, Liao, Guiyan, Li, Siqiao, Chen, Zhongliang, Liu, Xihui, Fang, Wenxia, Jin, Cheng, Wang, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266361/
https://www.ncbi.nlm.nih.gov/pubmed/35806213
http://dx.doi.org/10.3390/ijms23137202
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author Odiba, Arome Solomon
Ezechukwu, Chiemekam Samuel
Liao, Guiyan
Li, Siqiao
Chen, Zhongliang
Liu, Xihui
Fang, Wenxia
Jin, Cheng
Wang, Bin
author_facet Odiba, Arome Solomon
Ezechukwu, Chiemekam Samuel
Liao, Guiyan
Li, Siqiao
Chen, Zhongliang
Liu, Xihui
Fang, Wenxia
Jin, Cheng
Wang, Bin
author_sort Odiba, Arome Solomon
collection PubMed
description The Structural Maintenance of Chromosomes (SMC) complex plays an important role in maintaining chromosome integrity, in which the SMC5/6 complex occupies a central position by facilitating mitotic and meiotic processes as well as DNA repair. NSE-4 Kleisin is critical for both the organization and function of the SMC5/6 complex, bridging NSE1 and NSE3 (MAGE related) with the head domains of the SMC5 and SMC6 proteins. Despite the conservation in protein sequence, no functional relevance of the NSE-4 homologous protein (NSE-4) in Caenorhabditis elegans has been reported. Here, we demonstrated the essential role of C. elegans NSE-4 in genome maintenance and DNA repair. Our results showed that NSE-4 is essential for the maintenance of chromosomal structure and repair of a range of chemically induced DNA damage. Furthermore, NSE-4 is involved in inter-sister repair during meiosis. NSE-4 localizes on the chromosome and is indispensable for the localization of NSE-1. Collectively, our data from this study provide further insight into the evolutionary conservation and diversification of NSE-4 function in the SMC-5/6 complex.
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spelling pubmed-92663612022-07-09 Loss of NSE-4 Perturbs Genome Stability and DNA Repair in Caenorhabditis elegans Odiba, Arome Solomon Ezechukwu, Chiemekam Samuel Liao, Guiyan Li, Siqiao Chen, Zhongliang Liu, Xihui Fang, Wenxia Jin, Cheng Wang, Bin Int J Mol Sci Article The Structural Maintenance of Chromosomes (SMC) complex plays an important role in maintaining chromosome integrity, in which the SMC5/6 complex occupies a central position by facilitating mitotic and meiotic processes as well as DNA repair. NSE-4 Kleisin is critical for both the organization and function of the SMC5/6 complex, bridging NSE1 and NSE3 (MAGE related) with the head domains of the SMC5 and SMC6 proteins. Despite the conservation in protein sequence, no functional relevance of the NSE-4 homologous protein (NSE-4) in Caenorhabditis elegans has been reported. Here, we demonstrated the essential role of C. elegans NSE-4 in genome maintenance and DNA repair. Our results showed that NSE-4 is essential for the maintenance of chromosomal structure and repair of a range of chemically induced DNA damage. Furthermore, NSE-4 is involved in inter-sister repair during meiosis. NSE-4 localizes on the chromosome and is indispensable for the localization of NSE-1. Collectively, our data from this study provide further insight into the evolutionary conservation and diversification of NSE-4 function in the SMC-5/6 complex. MDPI 2022-06-29 /pmc/articles/PMC9266361/ /pubmed/35806213 http://dx.doi.org/10.3390/ijms23137202 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Odiba, Arome Solomon
Ezechukwu, Chiemekam Samuel
Liao, Guiyan
Li, Siqiao
Chen, Zhongliang
Liu, Xihui
Fang, Wenxia
Jin, Cheng
Wang, Bin
Loss of NSE-4 Perturbs Genome Stability and DNA Repair in Caenorhabditis elegans
title Loss of NSE-4 Perturbs Genome Stability and DNA Repair in Caenorhabditis elegans
title_full Loss of NSE-4 Perturbs Genome Stability and DNA Repair in Caenorhabditis elegans
title_fullStr Loss of NSE-4 Perturbs Genome Stability and DNA Repair in Caenorhabditis elegans
title_full_unstemmed Loss of NSE-4 Perturbs Genome Stability and DNA Repair in Caenorhabditis elegans
title_short Loss of NSE-4 Perturbs Genome Stability and DNA Repair in Caenorhabditis elegans
title_sort loss of nse-4 perturbs genome stability and dna repair in caenorhabditis elegans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266361/
https://www.ncbi.nlm.nih.gov/pubmed/35806213
http://dx.doi.org/10.3390/ijms23137202
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