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The Upregulated Expression of the Citrus RIN4 Gene in HLB Diseased Citrus Aids Candidatus Liberibacter Asiaticus Infection

The citrus industry has been threatened by Huanglongbing (HLB) for over a century. Here, an HLB-induced Arabidopsis RPM1-interacting protein 4 (RIN4) homologous gene was cloned from Citrus clementina, and its characteristics and function were analyzed to determine its role during citrus–Candidatus L...

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Autores principales: Cheng, Chunzhen, Zhong, Yun, Wang, Bin, Zhang, Yongyan, Wu, Huan, Jiang, Nonghui, Wu, Bo, Lv, Yuanda, Jiang, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266415/
https://www.ncbi.nlm.nih.gov/pubmed/35805971
http://dx.doi.org/10.3390/ijms23136971
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author Cheng, Chunzhen
Zhong, Yun
Wang, Bin
Zhang, Yongyan
Wu, Huan
Jiang, Nonghui
Wu, Bo
Lv, Yuanda
Jiang, Bo
author_facet Cheng, Chunzhen
Zhong, Yun
Wang, Bin
Zhang, Yongyan
Wu, Huan
Jiang, Nonghui
Wu, Bo
Lv, Yuanda
Jiang, Bo
author_sort Cheng, Chunzhen
collection PubMed
description The citrus industry has been threatened by Huanglongbing (HLB) for over a century. Here, an HLB-induced Arabidopsis RPM1-interacting protein 4 (RIN4) homologous gene was cloned from Citrus clementina, and its characteristics and function were analyzed to determine its role during citrus–Candidatus Liberibacter asiaticus (CLas) interactions. Quantitative real-time PCR showed that RIN4 was expressed in roots, stems, leaves and flowers, with the greatest expression level in leaves. Its expression was suppressed by gibberellic acid, indole-3-acetic acid, salicylic acid and jasmonic acid treatments, but was induced by abscisic acid and salt treatments, as well as wounding. The transient expression of a RIN4-GFP showed that RIN4 was localized in the cell membrane. RIN4-overexpressing transgenic C. maxima cv. ‘Shatianyou’ plants were obtained, and some transgenic plants showed greater sensitivity to CLas infection and earlier HLB symptoms appearance than non-transgenic controls. Results obtained in this study indicated that the upregulated expression of RIN4 in HLB diseased citrus may aid CLas infection.
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spelling pubmed-92664152022-07-09 The Upregulated Expression of the Citrus RIN4 Gene in HLB Diseased Citrus Aids Candidatus Liberibacter Asiaticus Infection Cheng, Chunzhen Zhong, Yun Wang, Bin Zhang, Yongyan Wu, Huan Jiang, Nonghui Wu, Bo Lv, Yuanda Jiang, Bo Int J Mol Sci Article The citrus industry has been threatened by Huanglongbing (HLB) for over a century. Here, an HLB-induced Arabidopsis RPM1-interacting protein 4 (RIN4) homologous gene was cloned from Citrus clementina, and its characteristics and function were analyzed to determine its role during citrus–Candidatus Liberibacter asiaticus (CLas) interactions. Quantitative real-time PCR showed that RIN4 was expressed in roots, stems, leaves and flowers, with the greatest expression level in leaves. Its expression was suppressed by gibberellic acid, indole-3-acetic acid, salicylic acid and jasmonic acid treatments, but was induced by abscisic acid and salt treatments, as well as wounding. The transient expression of a RIN4-GFP showed that RIN4 was localized in the cell membrane. RIN4-overexpressing transgenic C. maxima cv. ‘Shatianyou’ plants were obtained, and some transgenic plants showed greater sensitivity to CLas infection and earlier HLB symptoms appearance than non-transgenic controls. Results obtained in this study indicated that the upregulated expression of RIN4 in HLB diseased citrus may aid CLas infection. MDPI 2022-06-23 /pmc/articles/PMC9266415/ /pubmed/35805971 http://dx.doi.org/10.3390/ijms23136971 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cheng, Chunzhen
Zhong, Yun
Wang, Bin
Zhang, Yongyan
Wu, Huan
Jiang, Nonghui
Wu, Bo
Lv, Yuanda
Jiang, Bo
The Upregulated Expression of the Citrus RIN4 Gene in HLB Diseased Citrus Aids Candidatus Liberibacter Asiaticus Infection
title The Upregulated Expression of the Citrus RIN4 Gene in HLB Diseased Citrus Aids Candidatus Liberibacter Asiaticus Infection
title_full The Upregulated Expression of the Citrus RIN4 Gene in HLB Diseased Citrus Aids Candidatus Liberibacter Asiaticus Infection
title_fullStr The Upregulated Expression of the Citrus RIN4 Gene in HLB Diseased Citrus Aids Candidatus Liberibacter Asiaticus Infection
title_full_unstemmed The Upregulated Expression of the Citrus RIN4 Gene in HLB Diseased Citrus Aids Candidatus Liberibacter Asiaticus Infection
title_short The Upregulated Expression of the Citrus RIN4 Gene in HLB Diseased Citrus Aids Candidatus Liberibacter Asiaticus Infection
title_sort upregulated expression of the citrus rin4 gene in hlb diseased citrus aids candidatus liberibacter asiaticus infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266415/
https://www.ncbi.nlm.nih.gov/pubmed/35805971
http://dx.doi.org/10.3390/ijms23136971
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