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Enhanced Muscle Fibers of Epinephelus coioides by Myostatin Autologous Nucleic Acid Vaccine

Epinephelus coioides is a fish species with high economic value due to its delicious meat, high protein content, and rich fatty acid nutrition. It has become a high-economic fish in southern parts of China and some other Southeast Asian countries. In this study, the myostatin nucleic acid vaccine wa...

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Detalles Bibliográficos
Autores principales: Fu, Bing, Yang, Jinzeng, Yang, Yan, Xia, Jun, He, Yinglin, Wang, Qing, Zhao, Huihong, Yang, Huirong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266527/
https://www.ncbi.nlm.nih.gov/pubmed/35805999
http://dx.doi.org/10.3390/ijms23136997
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author Fu, Bing
Yang, Jinzeng
Yang, Yan
Xia, Jun
He, Yinglin
Wang, Qing
Zhao, Huihong
Yang, Huirong
author_facet Fu, Bing
Yang, Jinzeng
Yang, Yan
Xia, Jun
He, Yinglin
Wang, Qing
Zhao, Huihong
Yang, Huirong
author_sort Fu, Bing
collection PubMed
description Epinephelus coioides is a fish species with high economic value due to its delicious meat, high protein content, and rich fatty acid nutrition. It has become a high-economic fish in southern parts of China and some other Southeast Asian countries. In this study, the myostatin nucleic acid vaccine was constructed and used to immunize E. coioides. The results from body length and weight measurements indicated the myostatin nucleic acid vaccine promoted E. coioides growth performance by increasing muscle fiber size. The results from RT-qPCR analysis showed that myostatin nucleic acid vaccine upregulated the expression of myod, myog and p21 mRNA, downregulated the expression of smad3 and mrf4 mRNA. This preliminary study is the first report that explored the role of myostatin in E. coioides and showed positive effects of autologous nucleic acid vaccine on the muscle growth of E. coioides. Further experiments with increased numbers of animals and different doses are needed for its application to E. coiodes aquaculture production.
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spelling pubmed-92665272022-07-09 Enhanced Muscle Fibers of Epinephelus coioides by Myostatin Autologous Nucleic Acid Vaccine Fu, Bing Yang, Jinzeng Yang, Yan Xia, Jun He, Yinglin Wang, Qing Zhao, Huihong Yang, Huirong Int J Mol Sci Article Epinephelus coioides is a fish species with high economic value due to its delicious meat, high protein content, and rich fatty acid nutrition. It has become a high-economic fish in southern parts of China and some other Southeast Asian countries. In this study, the myostatin nucleic acid vaccine was constructed and used to immunize E. coioides. The results from body length and weight measurements indicated the myostatin nucleic acid vaccine promoted E. coioides growth performance by increasing muscle fiber size. The results from RT-qPCR analysis showed that myostatin nucleic acid vaccine upregulated the expression of myod, myog and p21 mRNA, downregulated the expression of smad3 and mrf4 mRNA. This preliminary study is the first report that explored the role of myostatin in E. coioides and showed positive effects of autologous nucleic acid vaccine on the muscle growth of E. coioides. Further experiments with increased numbers of animals and different doses are needed for its application to E. coiodes aquaculture production. MDPI 2022-06-23 /pmc/articles/PMC9266527/ /pubmed/35805999 http://dx.doi.org/10.3390/ijms23136997 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fu, Bing
Yang, Jinzeng
Yang, Yan
Xia, Jun
He, Yinglin
Wang, Qing
Zhao, Huihong
Yang, Huirong
Enhanced Muscle Fibers of Epinephelus coioides by Myostatin Autologous Nucleic Acid Vaccine
title Enhanced Muscle Fibers of Epinephelus coioides by Myostatin Autologous Nucleic Acid Vaccine
title_full Enhanced Muscle Fibers of Epinephelus coioides by Myostatin Autologous Nucleic Acid Vaccine
title_fullStr Enhanced Muscle Fibers of Epinephelus coioides by Myostatin Autologous Nucleic Acid Vaccine
title_full_unstemmed Enhanced Muscle Fibers of Epinephelus coioides by Myostatin Autologous Nucleic Acid Vaccine
title_short Enhanced Muscle Fibers of Epinephelus coioides by Myostatin Autologous Nucleic Acid Vaccine
title_sort enhanced muscle fibers of epinephelus coioides by myostatin autologous nucleic acid vaccine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266527/
https://www.ncbi.nlm.nih.gov/pubmed/35805999
http://dx.doi.org/10.3390/ijms23136997
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