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The effects of rivaroxaban, an oral anticoagulant, on human IVD primary cultures

INTRODUCTION: The present study aimed to investigate the potential effects of rivaroxaban, an oral anticoagulant that inhibits the effects of factor Xa, on intact intervertebral disc tissue cells and the extracellular matrix (ECM). MATERIAL AND METHODS: Rivaroxaban was applied to primary human cell...

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Autores principales: Caliskan, Tezcan, Akalan, Hande, Yilmaz, Ibrahim, Karaarslan, Numan, Sirin, Duygu Yasar, Ozbek, Hanefi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266726/
https://www.ncbi.nlm.nih.gov/pubmed/35832710
http://dx.doi.org/10.5114/aoms/136323
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author Caliskan, Tezcan
Akalan, Hande
Yilmaz, Ibrahim
Karaarslan, Numan
Sirin, Duygu Yasar
Ozbek, Hanefi
author_facet Caliskan, Tezcan
Akalan, Hande
Yilmaz, Ibrahim
Karaarslan, Numan
Sirin, Duygu Yasar
Ozbek, Hanefi
author_sort Caliskan, Tezcan
collection PubMed
description INTRODUCTION: The present study aimed to investigate the potential effects of rivaroxaban, an oral anticoagulant that inhibits the effects of factor Xa, on intact intervertebral disc tissue cells and the extracellular matrix (ECM). MATERIAL AND METHODS: Rivaroxaban was applied to primary human cell cultures prepared from tissues of the intervertebral disc. Comparative molecular analyses were performed on non-drug-treated control group samples. Descriptive statistics were presented as the mean ± standard deviation. An analysis of variance test was performed to determine whether there were significant differences in the mean across the groups. When differences across groups were observed, Tukey’s honestly significant difference post-hoc test was used for multiple pairwise comparisons. The significance of the obtained data was determined statistically. The α significance value was < 0.05. RESULTS: The cells in the control group and in the rivaroxaban-treated group were viable, healthy, and proliferated (p < 0.05). However, the expression levels of the chondroadherin gene (CHAD), cartilage oligo matrix protein (COMP), matrix metalloproteinase (MMP)-13, and MMP-19 genes were changed (p < 0.05). CONCLUSIONS: Although rivaroxaban does not suppress cell proliferation due to morphological, biological, and biochemical changes in the intervertebral disc tissue, it may change the expression of genes that are related to ECM maintenance.
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spelling pubmed-92667262022-07-12 The effects of rivaroxaban, an oral anticoagulant, on human IVD primary cultures Caliskan, Tezcan Akalan, Hande Yilmaz, Ibrahim Karaarslan, Numan Sirin, Duygu Yasar Ozbek, Hanefi Arch Med Sci Basic Research INTRODUCTION: The present study aimed to investigate the potential effects of rivaroxaban, an oral anticoagulant that inhibits the effects of factor Xa, on intact intervertebral disc tissue cells and the extracellular matrix (ECM). MATERIAL AND METHODS: Rivaroxaban was applied to primary human cell cultures prepared from tissues of the intervertebral disc. Comparative molecular analyses were performed on non-drug-treated control group samples. Descriptive statistics were presented as the mean ± standard deviation. An analysis of variance test was performed to determine whether there were significant differences in the mean across the groups. When differences across groups were observed, Tukey’s honestly significant difference post-hoc test was used for multiple pairwise comparisons. The significance of the obtained data was determined statistically. The α significance value was < 0.05. RESULTS: The cells in the control group and in the rivaroxaban-treated group were viable, healthy, and proliferated (p < 0.05). However, the expression levels of the chondroadherin gene (CHAD), cartilage oligo matrix protein (COMP), matrix metalloproteinase (MMP)-13, and MMP-19 genes were changed (p < 0.05). CONCLUSIONS: Although rivaroxaban does not suppress cell proliferation due to morphological, biological, and biochemical changes in the intervertebral disc tissue, it may change the expression of genes that are related to ECM maintenance. Termedia Publishing House 2021-05-09 /pmc/articles/PMC9266726/ /pubmed/35832710 http://dx.doi.org/10.5114/aoms/136323 Text en Copyright: © 2021 Termedia & Banach https://creativecommons.org/licenses/by-nc-sa/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
spellingShingle Basic Research
Caliskan, Tezcan
Akalan, Hande
Yilmaz, Ibrahim
Karaarslan, Numan
Sirin, Duygu Yasar
Ozbek, Hanefi
The effects of rivaroxaban, an oral anticoagulant, on human IVD primary cultures
title The effects of rivaroxaban, an oral anticoagulant, on human IVD primary cultures
title_full The effects of rivaroxaban, an oral anticoagulant, on human IVD primary cultures
title_fullStr The effects of rivaroxaban, an oral anticoagulant, on human IVD primary cultures
title_full_unstemmed The effects of rivaroxaban, an oral anticoagulant, on human IVD primary cultures
title_short The effects of rivaroxaban, an oral anticoagulant, on human IVD primary cultures
title_sort effects of rivaroxaban, an oral anticoagulant, on human ivd primary cultures
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266726/
https://www.ncbi.nlm.nih.gov/pubmed/35832710
http://dx.doi.org/10.5114/aoms/136323
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