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miRNA and mRNA Expression Profiles Associated with Lymph Node Metastasis and Prognosis in Penile Carcinoma

Penile cancer (PeC) is a rare disease, and no prognostic biomarkers have been adopted in clinical practice yet. The objective of the present study was to identify differentially expressed miRNAs (DEmiRs) and genes (DEGs) as potential biomarkers for lymph node metastasis and other prognostic factors...

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Autores principales: Murta, Claudio B., Furuya, Tatiane K., Carrasco, Alexis G. M., Uno, Miyuki, Sichero, Laura, Villa, Luisa L., Faraj, Sheila F., Coelho, Rafael F., Guglielmetti, Giuliano B., Cordeiro, Mauricio D., Leite, Katia R. M., Nahas, William C., Chammas, Roger, Pontes, José
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266734/
https://www.ncbi.nlm.nih.gov/pubmed/35806108
http://dx.doi.org/10.3390/ijms23137103
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author Murta, Claudio B.
Furuya, Tatiane K.
Carrasco, Alexis G. M.
Uno, Miyuki
Sichero, Laura
Villa, Luisa L.
Faraj, Sheila F.
Coelho, Rafael F.
Guglielmetti, Giuliano B.
Cordeiro, Mauricio D.
Leite, Katia R. M.
Nahas, William C.
Chammas, Roger
Pontes, José
author_facet Murta, Claudio B.
Furuya, Tatiane K.
Carrasco, Alexis G. M.
Uno, Miyuki
Sichero, Laura
Villa, Luisa L.
Faraj, Sheila F.
Coelho, Rafael F.
Guglielmetti, Giuliano B.
Cordeiro, Mauricio D.
Leite, Katia R. M.
Nahas, William C.
Chammas, Roger
Pontes, José
author_sort Murta, Claudio B.
collection PubMed
description Penile cancer (PeC) is a rare disease, and no prognostic biomarkers have been adopted in clinical practice yet. The objective of the present study was to identify differentially expressed miRNAs (DEmiRs) and genes (DEGs) as potential biomarkers for lymph node metastasis and other prognostic factors in PeC. Tumor samples were prospectively obtained from 24 patients with squamous cell carcinoma of the penis. miRNA microarray analysis was performed comparing tumors from patients with inguinal lymph node metastatic and localized disease, and the results were validated by qRT-PCR. Eighty-three gene expression levels were also compared between groups through qRT-PCR. Moreover, DEmiRs and DEGs expression levels were correlated with clinicopathological variables, cancer-specific (CSS), and overall survival (OS). TAC software, TM4 MeV 4.9 software, SPSS v.25.0, and R software v.4.0.2 were used for statistical analyses. We identified 21 DEmiRs in microarray analysis, and seven were selected for validation. miR-744-5p and miR-421 were overexpressed in tissue samples of metastatic patients, and high expression of miR-421 was also associated with lower OS. We found seven DEGs (CCND1, EGFR, ENTPD5, HOXA10, IGF1R, MYC, and SNAI2) related to metastatic disease. A significant association was found between increased MMP1 expression and tumor size, grade, pathological T stage, and perineural invasion. Other genes were also associated with clinicopathological variables, CSS and OS. Finally, we found changes in mRNA–miRNA regulation that contribute to understanding the mechanisms involved in tumor progression. Therefore, we identified miRNA and mRNA expression profiles as potential biomarkers associated with lymph node metastasis and prognosis in PeC, in addition to disruption in mRNA–miRNA regulation during disease progression.
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spelling pubmed-92667342022-07-09 miRNA and mRNA Expression Profiles Associated with Lymph Node Metastasis and Prognosis in Penile Carcinoma Murta, Claudio B. Furuya, Tatiane K. Carrasco, Alexis G. M. Uno, Miyuki Sichero, Laura Villa, Luisa L. Faraj, Sheila F. Coelho, Rafael F. Guglielmetti, Giuliano B. Cordeiro, Mauricio D. Leite, Katia R. M. Nahas, William C. Chammas, Roger Pontes, José Int J Mol Sci Article Penile cancer (PeC) is a rare disease, and no prognostic biomarkers have been adopted in clinical practice yet. The objective of the present study was to identify differentially expressed miRNAs (DEmiRs) and genes (DEGs) as potential biomarkers for lymph node metastasis and other prognostic factors in PeC. Tumor samples were prospectively obtained from 24 patients with squamous cell carcinoma of the penis. miRNA microarray analysis was performed comparing tumors from patients with inguinal lymph node metastatic and localized disease, and the results were validated by qRT-PCR. Eighty-three gene expression levels were also compared between groups through qRT-PCR. Moreover, DEmiRs and DEGs expression levels were correlated with clinicopathological variables, cancer-specific (CSS), and overall survival (OS). TAC software, TM4 MeV 4.9 software, SPSS v.25.0, and R software v.4.0.2 were used for statistical analyses. We identified 21 DEmiRs in microarray analysis, and seven were selected for validation. miR-744-5p and miR-421 were overexpressed in tissue samples of metastatic patients, and high expression of miR-421 was also associated with lower OS. We found seven DEGs (CCND1, EGFR, ENTPD5, HOXA10, IGF1R, MYC, and SNAI2) related to metastatic disease. A significant association was found between increased MMP1 expression and tumor size, grade, pathological T stage, and perineural invasion. Other genes were also associated with clinicopathological variables, CSS and OS. Finally, we found changes in mRNA–miRNA regulation that contribute to understanding the mechanisms involved in tumor progression. Therefore, we identified miRNA and mRNA expression profiles as potential biomarkers associated with lymph node metastasis and prognosis in PeC, in addition to disruption in mRNA–miRNA regulation during disease progression. MDPI 2022-06-26 /pmc/articles/PMC9266734/ /pubmed/35806108 http://dx.doi.org/10.3390/ijms23137103 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Murta, Claudio B.
Furuya, Tatiane K.
Carrasco, Alexis G. M.
Uno, Miyuki
Sichero, Laura
Villa, Luisa L.
Faraj, Sheila F.
Coelho, Rafael F.
Guglielmetti, Giuliano B.
Cordeiro, Mauricio D.
Leite, Katia R. M.
Nahas, William C.
Chammas, Roger
Pontes, José
miRNA and mRNA Expression Profiles Associated with Lymph Node Metastasis and Prognosis in Penile Carcinoma
title miRNA and mRNA Expression Profiles Associated with Lymph Node Metastasis and Prognosis in Penile Carcinoma
title_full miRNA and mRNA Expression Profiles Associated with Lymph Node Metastasis and Prognosis in Penile Carcinoma
title_fullStr miRNA and mRNA Expression Profiles Associated with Lymph Node Metastasis and Prognosis in Penile Carcinoma
title_full_unstemmed miRNA and mRNA Expression Profiles Associated with Lymph Node Metastasis and Prognosis in Penile Carcinoma
title_short miRNA and mRNA Expression Profiles Associated with Lymph Node Metastasis and Prognosis in Penile Carcinoma
title_sort mirna and mrna expression profiles associated with lymph node metastasis and prognosis in penile carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266734/
https://www.ncbi.nlm.nih.gov/pubmed/35806108
http://dx.doi.org/10.3390/ijms23137103
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