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Microsatellite Instability in Russian Patients with Colorectal Cancer
The aim of this study was to determine the characteristics of Russian patients with microsatellite instability (MSI) tumors. MSI in the tumor was determined in 514 patients with colon cancer using PCR and subsequent fragment analysis for five markers (NR21, NR24, BAT25, BAT26, and NR27). In the pres...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266820/ https://www.ncbi.nlm.nih.gov/pubmed/35806077 http://dx.doi.org/10.3390/ijms23137062 |
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author | Shubin, Vitaly Shelygin, Yury Achkasov, Sergey Sushkov, Oleg Nazarov, Ilya Ponomarenko, Alexey Alimova, Iuliia Loginova, Anna Tsukanov, Aleksey |
author_facet | Shubin, Vitaly Shelygin, Yury Achkasov, Sergey Sushkov, Oleg Nazarov, Ilya Ponomarenko, Alexey Alimova, Iuliia Loginova, Anna Tsukanov, Aleksey |
author_sort | Shubin, Vitaly |
collection | PubMed |
description | The aim of this study was to determine the characteristics of Russian patients with microsatellite instability (MSI) tumors. MSI in the tumor was determined in 514 patients with colon cancer using PCR and subsequent fragment analysis for five markers (NR21, NR24, BAT25, BAT26, and NR27). In the presence of microsatellite instability, the mismatch repair (MMR) system genes were examined using the NGS and MLPA methods to establish the diagnosis of Lynch syndrome. The overall frequency of MSI tumors was 15%: at stage I—19% (9/48), at stage II—21% (44/213), at stage III—16% (26/160), and at stage IV—2% (2/93). Patients with MSI tumors differed in the age of diagnosis, tumor localization, time of cancer recurrence, and stage of the disease. The overall and disease-free survival of patients whose tumors had MSI status was higher than that of patients with microsatellite-stable status, p = 0.04 and p = 0.02, respectively. Analysis of overall and disease-free survival of patients with Lynch syndrome and patients with sporadic colon cancer, but with MSI status, did not reveal significant differences, p = 0.52 and p = 0.24, respectively. The age of patients with Lynch syndrome was significantly younger than that of patients with sporadic colon cancer whose tumors had MSI status (p < 0.001). |
format | Online Article Text |
id | pubmed-9266820 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92668202022-07-09 Microsatellite Instability in Russian Patients with Colorectal Cancer Shubin, Vitaly Shelygin, Yury Achkasov, Sergey Sushkov, Oleg Nazarov, Ilya Ponomarenko, Alexey Alimova, Iuliia Loginova, Anna Tsukanov, Aleksey Int J Mol Sci Article The aim of this study was to determine the characteristics of Russian patients with microsatellite instability (MSI) tumors. MSI in the tumor was determined in 514 patients with colon cancer using PCR and subsequent fragment analysis for five markers (NR21, NR24, BAT25, BAT26, and NR27). In the presence of microsatellite instability, the mismatch repair (MMR) system genes were examined using the NGS and MLPA methods to establish the diagnosis of Lynch syndrome. The overall frequency of MSI tumors was 15%: at stage I—19% (9/48), at stage II—21% (44/213), at stage III—16% (26/160), and at stage IV—2% (2/93). Patients with MSI tumors differed in the age of diagnosis, tumor localization, time of cancer recurrence, and stage of the disease. The overall and disease-free survival of patients whose tumors had MSI status was higher than that of patients with microsatellite-stable status, p = 0.04 and p = 0.02, respectively. Analysis of overall and disease-free survival of patients with Lynch syndrome and patients with sporadic colon cancer, but with MSI status, did not reveal significant differences, p = 0.52 and p = 0.24, respectively. The age of patients with Lynch syndrome was significantly younger than that of patients with sporadic colon cancer whose tumors had MSI status (p < 0.001). MDPI 2022-06-25 /pmc/articles/PMC9266820/ /pubmed/35806077 http://dx.doi.org/10.3390/ijms23137062 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shubin, Vitaly Shelygin, Yury Achkasov, Sergey Sushkov, Oleg Nazarov, Ilya Ponomarenko, Alexey Alimova, Iuliia Loginova, Anna Tsukanov, Aleksey Microsatellite Instability in Russian Patients with Colorectal Cancer |
title | Microsatellite Instability in Russian Patients with Colorectal Cancer |
title_full | Microsatellite Instability in Russian Patients with Colorectal Cancer |
title_fullStr | Microsatellite Instability in Russian Patients with Colorectal Cancer |
title_full_unstemmed | Microsatellite Instability in Russian Patients with Colorectal Cancer |
title_short | Microsatellite Instability in Russian Patients with Colorectal Cancer |
title_sort | microsatellite instability in russian patients with colorectal cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266820/ https://www.ncbi.nlm.nih.gov/pubmed/35806077 http://dx.doi.org/10.3390/ijms23137062 |
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