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Overexpression of OsGF14f Enhances Quantitative Leaf Blast and Bacterial Blight Resistance in Rice

Although it is known that rice 14-3-3 family genes are involved in various defense responses, the functions of OsGF14f in response to diseases have not been reported. Here, we showed that the transcription of OsGF14f was significantly induced by leaf blast infection, and the overexpression of OsGF14...

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Autores principales: Ma, Yamei, Yang, Jianyuan, Dong, Jingfang, Zhang, Shaohong, Yang, Wu, Zhao, Junliang, Yang, Tifeng, Chen, Luo, Zhou, Lian, Wang, Jian, Chen, Jiansong, Li, Wenhui, Wu, Wei, Liu, Qing, Liu, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266906/
https://www.ncbi.nlm.nih.gov/pubmed/35806444
http://dx.doi.org/10.3390/ijms23137440
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author Ma, Yamei
Yang, Jianyuan
Dong, Jingfang
Zhang, Shaohong
Yang, Wu
Zhao, Junliang
Yang, Tifeng
Chen, Luo
Zhou, Lian
Wang, Jian
Chen, Jiansong
Li, Wenhui
Wu, Wei
Liu, Qing
Liu, Bin
author_facet Ma, Yamei
Yang, Jianyuan
Dong, Jingfang
Zhang, Shaohong
Yang, Wu
Zhao, Junliang
Yang, Tifeng
Chen, Luo
Zhou, Lian
Wang, Jian
Chen, Jiansong
Li, Wenhui
Wu, Wei
Liu, Qing
Liu, Bin
author_sort Ma, Yamei
collection PubMed
description Although it is known that rice 14-3-3 family genes are involved in various defense responses, the functions of OsGF14f in response to diseases have not been reported. Here, we showed that the transcription of OsGF14f was significantly induced by leaf blast infection, and the overexpression of OsGF14f quantitatively enhanced resistance to leaf blast and bacterial blight in rice. Further analysis showed that the expression levels of salicylic acid (SA) pathway-associated genes (PAL1, NH1, PR1a and PR10) in the OsGF14f-overexpressing plants, were higher than those in wild-type plants after inoculation with the blast isolate (Magnaporthe oryzae Barr). In addition, the expression level of OsGF14f was significantly induced after SA treatment, and higher endogenous SA levels were observed in the OsGF14f-overexpressing plants compared with that in wild-type plants, especially after blast challenge. Taken together, these results suggest that OsGF14f positively regulates leaf blast and bacterial blight resistance in rice via the SA-dependent signaling pathway.
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spelling pubmed-92669062022-07-09 Overexpression of OsGF14f Enhances Quantitative Leaf Blast and Bacterial Blight Resistance in Rice Ma, Yamei Yang, Jianyuan Dong, Jingfang Zhang, Shaohong Yang, Wu Zhao, Junliang Yang, Tifeng Chen, Luo Zhou, Lian Wang, Jian Chen, Jiansong Li, Wenhui Wu, Wei Liu, Qing Liu, Bin Int J Mol Sci Article Although it is known that rice 14-3-3 family genes are involved in various defense responses, the functions of OsGF14f in response to diseases have not been reported. Here, we showed that the transcription of OsGF14f was significantly induced by leaf blast infection, and the overexpression of OsGF14f quantitatively enhanced resistance to leaf blast and bacterial blight in rice. Further analysis showed that the expression levels of salicylic acid (SA) pathway-associated genes (PAL1, NH1, PR1a and PR10) in the OsGF14f-overexpressing plants, were higher than those in wild-type plants after inoculation with the blast isolate (Magnaporthe oryzae Barr). In addition, the expression level of OsGF14f was significantly induced after SA treatment, and higher endogenous SA levels were observed in the OsGF14f-overexpressing plants compared with that in wild-type plants, especially after blast challenge. Taken together, these results suggest that OsGF14f positively regulates leaf blast and bacterial blight resistance in rice via the SA-dependent signaling pathway. MDPI 2022-07-04 /pmc/articles/PMC9266906/ /pubmed/35806444 http://dx.doi.org/10.3390/ijms23137440 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ma, Yamei
Yang, Jianyuan
Dong, Jingfang
Zhang, Shaohong
Yang, Wu
Zhao, Junliang
Yang, Tifeng
Chen, Luo
Zhou, Lian
Wang, Jian
Chen, Jiansong
Li, Wenhui
Wu, Wei
Liu, Qing
Liu, Bin
Overexpression of OsGF14f Enhances Quantitative Leaf Blast and Bacterial Blight Resistance in Rice
title Overexpression of OsGF14f Enhances Quantitative Leaf Blast and Bacterial Blight Resistance in Rice
title_full Overexpression of OsGF14f Enhances Quantitative Leaf Blast and Bacterial Blight Resistance in Rice
title_fullStr Overexpression of OsGF14f Enhances Quantitative Leaf Blast and Bacterial Blight Resistance in Rice
title_full_unstemmed Overexpression of OsGF14f Enhances Quantitative Leaf Blast and Bacterial Blight Resistance in Rice
title_short Overexpression of OsGF14f Enhances Quantitative Leaf Blast and Bacterial Blight Resistance in Rice
title_sort overexpression of osgf14f enhances quantitative leaf blast and bacterial blight resistance in rice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266906/
https://www.ncbi.nlm.nih.gov/pubmed/35806444
http://dx.doi.org/10.3390/ijms23137440
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