Cargando…

Antimicrobial Combined Action of Graphene Oxide and Light Emitting Diodes for Chronic Wound Management

Innovative non-antibiotic compounds such as graphene oxide (GO) and light-emitting diodes (LEDs) may represent a valid strategy for managing chronic wound infections related to resistant pathogens. This study aimed to evaluate 630 nm LED and 880 nm LED ability to enhance the GO antimicrobial activit...

Descripción completa

Detalles Bibliográficos
Autores principales: Di Lodovico, Silvia, Diban, Firas, Di Fermo, Paola, Petrini, Morena, Fontana, Antonella, Di Giulio, Mara, Piattelli, Adriano, D’Ercole, Simonetta, Cellini, Luigina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266944/
https://www.ncbi.nlm.nih.gov/pubmed/35805944
http://dx.doi.org/10.3390/ijms23136942
_version_ 1784743594821681152
author Di Lodovico, Silvia
Diban, Firas
Di Fermo, Paola
Petrini, Morena
Fontana, Antonella
Di Giulio, Mara
Piattelli, Adriano
D’Ercole, Simonetta
Cellini, Luigina
author_facet Di Lodovico, Silvia
Diban, Firas
Di Fermo, Paola
Petrini, Morena
Fontana, Antonella
Di Giulio, Mara
Piattelli, Adriano
D’Ercole, Simonetta
Cellini, Luigina
author_sort Di Lodovico, Silvia
collection PubMed
description Innovative non-antibiotic compounds such as graphene oxide (GO) and light-emitting diodes (LEDs) may represent a valid strategy for managing chronic wound infections related to resistant pathogens. This study aimed to evaluate 630 nm LED and 880 nm LED ability to enhance the GO antimicrobial activity against Staphylococcus aureus- and Pseudomonas aeruginosa-resistant strains in a dual-species biofilm in the Lubbock chronic wound biofilm (LCWB) model. The effect of a 630 nm LED, alone or plus 5-aminolevulinic acid (ALAD)-mediated photodynamic therapy (PDT) (ALAD-PDT), or an 880 nm LED on the GO (50 mg/l) action was evaluated by determining the CFU/mg reductions, live/dead analysis, scanning electron microscope observation, and reactive oxygen species assay. Among the LCWBs, the best effect was obtained with GO irradiated with ALAD-PDT, with percentages of CFU/mg reduction up to 78.96% ± 0.21 and 95.17% ± 2.56 for S. aureus and P. aeruginosa, respectively. The microscope images showed a reduction in the cell number and viability when treated with GO + ALAD-PDT. In addition, increased ROS production was detected. No differences were recorded when GO was irradiated with an 880 nm LED versus GO alone. The obtained results suggest that treatment with GO irradiated with ALAD-PDT represents a valid, sustainable strategy to counteract the polymicrobial colonization of chronic wounds.
format Online
Article
Text
id pubmed-9266944
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-92669442022-07-09 Antimicrobial Combined Action of Graphene Oxide and Light Emitting Diodes for Chronic Wound Management Di Lodovico, Silvia Diban, Firas Di Fermo, Paola Petrini, Morena Fontana, Antonella Di Giulio, Mara Piattelli, Adriano D’Ercole, Simonetta Cellini, Luigina Int J Mol Sci Article Innovative non-antibiotic compounds such as graphene oxide (GO) and light-emitting diodes (LEDs) may represent a valid strategy for managing chronic wound infections related to resistant pathogens. This study aimed to evaluate 630 nm LED and 880 nm LED ability to enhance the GO antimicrobial activity against Staphylococcus aureus- and Pseudomonas aeruginosa-resistant strains in a dual-species biofilm in the Lubbock chronic wound biofilm (LCWB) model. The effect of a 630 nm LED, alone or plus 5-aminolevulinic acid (ALAD)-mediated photodynamic therapy (PDT) (ALAD-PDT), or an 880 nm LED on the GO (50 mg/l) action was evaluated by determining the CFU/mg reductions, live/dead analysis, scanning electron microscope observation, and reactive oxygen species assay. Among the LCWBs, the best effect was obtained with GO irradiated with ALAD-PDT, with percentages of CFU/mg reduction up to 78.96% ± 0.21 and 95.17% ± 2.56 for S. aureus and P. aeruginosa, respectively. The microscope images showed a reduction in the cell number and viability when treated with GO + ALAD-PDT. In addition, increased ROS production was detected. No differences were recorded when GO was irradiated with an 880 nm LED versus GO alone. The obtained results suggest that treatment with GO irradiated with ALAD-PDT represents a valid, sustainable strategy to counteract the polymicrobial colonization of chronic wounds. MDPI 2022-06-22 /pmc/articles/PMC9266944/ /pubmed/35805944 http://dx.doi.org/10.3390/ijms23136942 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Di Lodovico, Silvia
Diban, Firas
Di Fermo, Paola
Petrini, Morena
Fontana, Antonella
Di Giulio, Mara
Piattelli, Adriano
D’Ercole, Simonetta
Cellini, Luigina
Antimicrobial Combined Action of Graphene Oxide and Light Emitting Diodes for Chronic Wound Management
title Antimicrobial Combined Action of Graphene Oxide and Light Emitting Diodes for Chronic Wound Management
title_full Antimicrobial Combined Action of Graphene Oxide and Light Emitting Diodes for Chronic Wound Management
title_fullStr Antimicrobial Combined Action of Graphene Oxide and Light Emitting Diodes for Chronic Wound Management
title_full_unstemmed Antimicrobial Combined Action of Graphene Oxide and Light Emitting Diodes for Chronic Wound Management
title_short Antimicrobial Combined Action of Graphene Oxide and Light Emitting Diodes for Chronic Wound Management
title_sort antimicrobial combined action of graphene oxide and light emitting diodes for chronic wound management
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9266944/
https://www.ncbi.nlm.nih.gov/pubmed/35805944
http://dx.doi.org/10.3390/ijms23136942
work_keys_str_mv AT dilodovicosilvia antimicrobialcombinedactionofgrapheneoxideandlightemittingdiodesforchronicwoundmanagement
AT dibanfiras antimicrobialcombinedactionofgrapheneoxideandlightemittingdiodesforchronicwoundmanagement
AT difermopaola antimicrobialcombinedactionofgrapheneoxideandlightemittingdiodesforchronicwoundmanagement
AT petrinimorena antimicrobialcombinedactionofgrapheneoxideandlightemittingdiodesforchronicwoundmanagement
AT fontanaantonella antimicrobialcombinedactionofgrapheneoxideandlightemittingdiodesforchronicwoundmanagement
AT digiuliomara antimicrobialcombinedactionofgrapheneoxideandlightemittingdiodesforchronicwoundmanagement
AT piattelliadriano antimicrobialcombinedactionofgrapheneoxideandlightemittingdiodesforchronicwoundmanagement
AT dercolesimonetta antimicrobialcombinedactionofgrapheneoxideandlightemittingdiodesforchronicwoundmanagement
AT celliniluigina antimicrobialcombinedactionofgrapheneoxideandlightemittingdiodesforchronicwoundmanagement