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In Vitro and Ectopic In Vivo Studies toward the Utilization of Rapidly Isolated Human Nasal Chondrocytes for Single-Stage Arthroscopic Cartilage Regeneration Therapy

Nasal chondrocytes (NCs) have a higher and more reproducible chondrogenic capacity than articular chondrocytes, and the engineered cartilage tissue they generate in vitro has been demonstrated to be safe in clinical applications. Here, we aimed at determining the feasibility for a single-stage appli...

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Autores principales: Lehoczky, Gyözö, Trofin, Raluca Elena, Vallmajo-Martin, Queralt, Chawla, Shikha, Pelttari, Karoliina, Mumme, Marcus, Haug, Martin, Egloff, Christian, Jakob, Marcel, Ehrbar, Martin, Martin, Ivan, Barbero, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9267018/
https://www.ncbi.nlm.nih.gov/pubmed/35805907
http://dx.doi.org/10.3390/ijms23136900
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author Lehoczky, Gyözö
Trofin, Raluca Elena
Vallmajo-Martin, Queralt
Chawla, Shikha
Pelttari, Karoliina
Mumme, Marcus
Haug, Martin
Egloff, Christian
Jakob, Marcel
Ehrbar, Martin
Martin, Ivan
Barbero, Andrea
author_facet Lehoczky, Gyözö
Trofin, Raluca Elena
Vallmajo-Martin, Queralt
Chawla, Shikha
Pelttari, Karoliina
Mumme, Marcus
Haug, Martin
Egloff, Christian
Jakob, Marcel
Ehrbar, Martin
Martin, Ivan
Barbero, Andrea
author_sort Lehoczky, Gyözö
collection PubMed
description Nasal chondrocytes (NCs) have a higher and more reproducible chondrogenic capacity than articular chondrocytes, and the engineered cartilage tissue they generate in vitro has been demonstrated to be safe in clinical applications. Here, we aimed at determining the feasibility for a single-stage application of NCs for cartilage regeneration under minimally invasive settings. In particular, we assessed whether NCs isolated using a short collagenase digestion protocol retain their potential to proliferate and chondro-differentiate within an injectable, swiftly cross-linked and matrix-metalloproteinase (MMP)-degradable polyethylene glycol (PEG) gel enriched with human platelet lysate (hPL). NC-hPL-PEG gels were additionally tested for their capacity to generate cartilage tissue in vivo and to integrate into cartilage/bone compartments of human osteochondral plugs upon ectopic subcutaneous implantation into nude mice. NCs isolated with a rapid protocol and embedded in PEG gels with hPL at low cell density were capable of efficiently proliferating and of generating tissue rich in glycosaminoglycans and collagen II. NC-hPL-PEG gels developed into hyaline-like cartilage tissues upon ectopic in vivo implantation and integrated with surrounding native cartilage and bone tissues. The delivery of NCs in PEG gels containing hPL is a feasible strategy for cartilage repair and now requires further validation in orthotopic in vivo models.
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spelling pubmed-92670182022-07-09 In Vitro and Ectopic In Vivo Studies toward the Utilization of Rapidly Isolated Human Nasal Chondrocytes for Single-Stage Arthroscopic Cartilage Regeneration Therapy Lehoczky, Gyözö Trofin, Raluca Elena Vallmajo-Martin, Queralt Chawla, Shikha Pelttari, Karoliina Mumme, Marcus Haug, Martin Egloff, Christian Jakob, Marcel Ehrbar, Martin Martin, Ivan Barbero, Andrea Int J Mol Sci Article Nasal chondrocytes (NCs) have a higher and more reproducible chondrogenic capacity than articular chondrocytes, and the engineered cartilage tissue they generate in vitro has been demonstrated to be safe in clinical applications. Here, we aimed at determining the feasibility for a single-stage application of NCs for cartilage regeneration under minimally invasive settings. In particular, we assessed whether NCs isolated using a short collagenase digestion protocol retain their potential to proliferate and chondro-differentiate within an injectable, swiftly cross-linked and matrix-metalloproteinase (MMP)-degradable polyethylene glycol (PEG) gel enriched with human platelet lysate (hPL). NC-hPL-PEG gels were additionally tested for their capacity to generate cartilage tissue in vivo and to integrate into cartilage/bone compartments of human osteochondral plugs upon ectopic subcutaneous implantation into nude mice. NCs isolated with a rapid protocol and embedded in PEG gels with hPL at low cell density were capable of efficiently proliferating and of generating tissue rich in glycosaminoglycans and collagen II. NC-hPL-PEG gels developed into hyaline-like cartilage tissues upon ectopic in vivo implantation and integrated with surrounding native cartilage and bone tissues. The delivery of NCs in PEG gels containing hPL is a feasible strategy for cartilage repair and now requires further validation in orthotopic in vivo models. MDPI 2022-06-21 /pmc/articles/PMC9267018/ /pubmed/35805907 http://dx.doi.org/10.3390/ijms23136900 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lehoczky, Gyözö
Trofin, Raluca Elena
Vallmajo-Martin, Queralt
Chawla, Shikha
Pelttari, Karoliina
Mumme, Marcus
Haug, Martin
Egloff, Christian
Jakob, Marcel
Ehrbar, Martin
Martin, Ivan
Barbero, Andrea
In Vitro and Ectopic In Vivo Studies toward the Utilization of Rapidly Isolated Human Nasal Chondrocytes for Single-Stage Arthroscopic Cartilage Regeneration Therapy
title In Vitro and Ectopic In Vivo Studies toward the Utilization of Rapidly Isolated Human Nasal Chondrocytes for Single-Stage Arthroscopic Cartilage Regeneration Therapy
title_full In Vitro and Ectopic In Vivo Studies toward the Utilization of Rapidly Isolated Human Nasal Chondrocytes for Single-Stage Arthroscopic Cartilage Regeneration Therapy
title_fullStr In Vitro and Ectopic In Vivo Studies toward the Utilization of Rapidly Isolated Human Nasal Chondrocytes for Single-Stage Arthroscopic Cartilage Regeneration Therapy
title_full_unstemmed In Vitro and Ectopic In Vivo Studies toward the Utilization of Rapidly Isolated Human Nasal Chondrocytes for Single-Stage Arthroscopic Cartilage Regeneration Therapy
title_short In Vitro and Ectopic In Vivo Studies toward the Utilization of Rapidly Isolated Human Nasal Chondrocytes for Single-Stage Arthroscopic Cartilage Regeneration Therapy
title_sort in vitro and ectopic in vivo studies toward the utilization of rapidly isolated human nasal chondrocytes for single-stage arthroscopic cartilage regeneration therapy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9267018/
https://www.ncbi.nlm.nih.gov/pubmed/35805907
http://dx.doi.org/10.3390/ijms23136900
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