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Electrospun Membranes Designed for Burst Release of New Gold-Complexes Inducing Apoptosis of Melanoma Cells

Two non-commercial metallic Au-based complexes were tested against one of the most aggressive malignant melanomas of the skin (MeWo cells), through cell viability and time-lapse live-cell imaging system assays. The tests with the complexes were carried out both in the form of free metallic complexes...

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Autores principales: Guadagno, Liberata, Raimondo, Marialuigia, Vertuccio, Luigi, Lamparelli, Erwin Pavel, Ciardulli, Maria Camilla, Longo, Pasquale, Mariconda, Annaluisa, Della Porta, Giovanna, Longo, Raffaele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9267035/
https://www.ncbi.nlm.nih.gov/pubmed/35806152
http://dx.doi.org/10.3390/ijms23137147
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author Guadagno, Liberata
Raimondo, Marialuigia
Vertuccio, Luigi
Lamparelli, Erwin Pavel
Ciardulli, Maria Camilla
Longo, Pasquale
Mariconda, Annaluisa
Della Porta, Giovanna
Longo, Raffaele
author_facet Guadagno, Liberata
Raimondo, Marialuigia
Vertuccio, Luigi
Lamparelli, Erwin Pavel
Ciardulli, Maria Camilla
Longo, Pasquale
Mariconda, Annaluisa
Della Porta, Giovanna
Longo, Raffaele
author_sort Guadagno, Liberata
collection PubMed
description Two non-commercial metallic Au-based complexes were tested against one of the most aggressive malignant melanomas of the skin (MeWo cells), through cell viability and time-lapse live-cell imaging system assays. The tests with the complexes were carried out both in the form of free metallic complexes, directly in contact with the MeWo cell line culture, and embedded in fibers of Polycaprolactone (PCL) membranes produced by the electrospinning technique. Membranes functionalized with complexes were prepared to evaluate the efficiency of the membranes against the melanoma cells and therefore their feasibility in the application as an antitumoral patch for topical use. Both series of tests highlighted a very effective antitumoral activity, manifesting a very relevant cell viability inhibition after both 24 h and 48 h. In the case of the AuM1 complex at the concentration of 20 mM, melanoma cells completely died in this short period of time. A mortality of around 70% was detected from the tests performed using the membranes functionalized with AuM1 complex at a very low concentration (3 wt.%), even after 24 h of the contact period. The synthesized complexes also manifest high selectivity with respect to the MeWo cells. The peculiar structural and morphological organization of the nanofibers constituting the membranes allows for a very effective antitumoral activity in the first 3 h of treatment. Experimental points of the release profiles were perfectly fitted with theoretical curves, which easily allow interpretation of the kinetic phenomena occurring in the release of the synthesized complexes in the chosen medium.
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spelling pubmed-92670352022-07-09 Electrospun Membranes Designed for Burst Release of New Gold-Complexes Inducing Apoptosis of Melanoma Cells Guadagno, Liberata Raimondo, Marialuigia Vertuccio, Luigi Lamparelli, Erwin Pavel Ciardulli, Maria Camilla Longo, Pasquale Mariconda, Annaluisa Della Porta, Giovanna Longo, Raffaele Int J Mol Sci Article Two non-commercial metallic Au-based complexes were tested against one of the most aggressive malignant melanomas of the skin (MeWo cells), through cell viability and time-lapse live-cell imaging system assays. The tests with the complexes were carried out both in the form of free metallic complexes, directly in contact with the MeWo cell line culture, and embedded in fibers of Polycaprolactone (PCL) membranes produced by the electrospinning technique. Membranes functionalized with complexes were prepared to evaluate the efficiency of the membranes against the melanoma cells and therefore their feasibility in the application as an antitumoral patch for topical use. Both series of tests highlighted a very effective antitumoral activity, manifesting a very relevant cell viability inhibition after both 24 h and 48 h. In the case of the AuM1 complex at the concentration of 20 mM, melanoma cells completely died in this short period of time. A mortality of around 70% was detected from the tests performed using the membranes functionalized with AuM1 complex at a very low concentration (3 wt.%), even after 24 h of the contact period. The synthesized complexes also manifest high selectivity with respect to the MeWo cells. The peculiar structural and morphological organization of the nanofibers constituting the membranes allows for a very effective antitumoral activity in the first 3 h of treatment. Experimental points of the release profiles were perfectly fitted with theoretical curves, which easily allow interpretation of the kinetic phenomena occurring in the release of the synthesized complexes in the chosen medium. MDPI 2022-06-27 /pmc/articles/PMC9267035/ /pubmed/35806152 http://dx.doi.org/10.3390/ijms23137147 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guadagno, Liberata
Raimondo, Marialuigia
Vertuccio, Luigi
Lamparelli, Erwin Pavel
Ciardulli, Maria Camilla
Longo, Pasquale
Mariconda, Annaluisa
Della Porta, Giovanna
Longo, Raffaele
Electrospun Membranes Designed for Burst Release of New Gold-Complexes Inducing Apoptosis of Melanoma Cells
title Electrospun Membranes Designed for Burst Release of New Gold-Complexes Inducing Apoptosis of Melanoma Cells
title_full Electrospun Membranes Designed for Burst Release of New Gold-Complexes Inducing Apoptosis of Melanoma Cells
title_fullStr Electrospun Membranes Designed for Burst Release of New Gold-Complexes Inducing Apoptosis of Melanoma Cells
title_full_unstemmed Electrospun Membranes Designed for Burst Release of New Gold-Complexes Inducing Apoptosis of Melanoma Cells
title_short Electrospun Membranes Designed for Burst Release of New Gold-Complexes Inducing Apoptosis of Melanoma Cells
title_sort electrospun membranes designed for burst release of new gold-complexes inducing apoptosis of melanoma cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9267035/
https://www.ncbi.nlm.nih.gov/pubmed/35806152
http://dx.doi.org/10.3390/ijms23137147
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