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Identification of Type-H-like Blood Vessels in a Dynamic and Controlled Model of Osteogenesis in Rabbit Calvarium

Angiogenesis and bone regeneration are closely interconnected processes. Whereas type-H blood vessels are abundantly found in the osteogenic zones during endochondral long bone development, their presence in flat bones’ development involving intramembranous mechanisms remains unclear. Here, we hypot...

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Autores principales: Marger, Laurine, Liaudet, Nicolas, Scherrer, Susanne S., Brembilla, Nicolo-Constantino, Preynat-Seauve, Olivier, Manoil, Daniel, Mekki, Mustapha, Durual, Stéphane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9267487/
https://www.ncbi.nlm.nih.gov/pubmed/35806828
http://dx.doi.org/10.3390/ma15134703
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author Marger, Laurine
Liaudet, Nicolas
Scherrer, Susanne S.
Brembilla, Nicolo-Constantino
Preynat-Seauve, Olivier
Manoil, Daniel
Mekki, Mustapha
Durual, Stéphane
author_facet Marger, Laurine
Liaudet, Nicolas
Scherrer, Susanne S.
Brembilla, Nicolo-Constantino
Preynat-Seauve, Olivier
Manoil, Daniel
Mekki, Mustapha
Durual, Stéphane
author_sort Marger, Laurine
collection PubMed
description Angiogenesis and bone regeneration are closely interconnected processes. Whereas type-H blood vessels are abundantly found in the osteogenic zones during endochondral long bone development, their presence in flat bones’ development involving intramembranous mechanisms remains unclear. Here, we hypothesized that type-H-like capillaries that highly express CD31 and Endomucin (EMCN), may be present at sites of intramembranous bone development and participate in the control of osteogenesis. A rabbit model of calvarial bone augmentation was used in which bone growth was controlled over time (2–4 weeks) using a particulate bone scaffold. The model allowed the visualization of the entire spectrum of stages throughout bone growth in the same sample, i.e., active ossification, osteogenic activity, and controlled inflammation. Using systematic mRNA hybridization, the formation of capillaries subpopulations (CD31–EMCN staining) over time was studied and correlated with the presence of osteogenic precursors (Osterix staining). Type-H-like capillaries strongly expressing CD31 and EMCN were identified and described. Their presence increased gradually from the regenerative zone up to the osteogenic zone, at 2 and 4 weeks. Type-H-like capillaries may thus represent the initial vascular support encountered in flat bones’ development and which organize osteogenic niches.
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spelling pubmed-92674872022-07-09 Identification of Type-H-like Blood Vessels in a Dynamic and Controlled Model of Osteogenesis in Rabbit Calvarium Marger, Laurine Liaudet, Nicolas Scherrer, Susanne S. Brembilla, Nicolo-Constantino Preynat-Seauve, Olivier Manoil, Daniel Mekki, Mustapha Durual, Stéphane Materials (Basel) Article Angiogenesis and bone regeneration are closely interconnected processes. Whereas type-H blood vessels are abundantly found in the osteogenic zones during endochondral long bone development, their presence in flat bones’ development involving intramembranous mechanisms remains unclear. Here, we hypothesized that type-H-like capillaries that highly express CD31 and Endomucin (EMCN), may be present at sites of intramembranous bone development and participate in the control of osteogenesis. A rabbit model of calvarial bone augmentation was used in which bone growth was controlled over time (2–4 weeks) using a particulate bone scaffold. The model allowed the visualization of the entire spectrum of stages throughout bone growth in the same sample, i.e., active ossification, osteogenic activity, and controlled inflammation. Using systematic mRNA hybridization, the formation of capillaries subpopulations (CD31–EMCN staining) over time was studied and correlated with the presence of osteogenic precursors (Osterix staining). Type-H-like capillaries strongly expressing CD31 and EMCN were identified and described. Their presence increased gradually from the regenerative zone up to the osteogenic zone, at 2 and 4 weeks. Type-H-like capillaries may thus represent the initial vascular support encountered in flat bones’ development and which organize osteogenic niches. MDPI 2022-07-05 /pmc/articles/PMC9267487/ /pubmed/35806828 http://dx.doi.org/10.3390/ma15134703 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Marger, Laurine
Liaudet, Nicolas
Scherrer, Susanne S.
Brembilla, Nicolo-Constantino
Preynat-Seauve, Olivier
Manoil, Daniel
Mekki, Mustapha
Durual, Stéphane
Identification of Type-H-like Blood Vessels in a Dynamic and Controlled Model of Osteogenesis in Rabbit Calvarium
title Identification of Type-H-like Blood Vessels in a Dynamic and Controlled Model of Osteogenesis in Rabbit Calvarium
title_full Identification of Type-H-like Blood Vessels in a Dynamic and Controlled Model of Osteogenesis in Rabbit Calvarium
title_fullStr Identification of Type-H-like Blood Vessels in a Dynamic and Controlled Model of Osteogenesis in Rabbit Calvarium
title_full_unstemmed Identification of Type-H-like Blood Vessels in a Dynamic and Controlled Model of Osteogenesis in Rabbit Calvarium
title_short Identification of Type-H-like Blood Vessels in a Dynamic and Controlled Model of Osteogenesis in Rabbit Calvarium
title_sort identification of type-h-like blood vessels in a dynamic and controlled model of osteogenesis in rabbit calvarium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9267487/
https://www.ncbi.nlm.nih.gov/pubmed/35806828
http://dx.doi.org/10.3390/ma15134703
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