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Fluorescent Nitrogen-Doped Carbon Dots for Label Live Elder Blood-Stage Plasmodium falciparum through New Permeability Pathways
To verify the size and emergence time of new permeability pathways (NPPs) in malaria parasites, the permeability of the Plasmodium falciparum-infected erythrocytes was tested with different particle sizes of nanomaterials by flow cytometry assay. The results confirmed the permeability of the host ce...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9267939/ https://www.ncbi.nlm.nih.gov/pubmed/35807422 http://dx.doi.org/10.3390/molecules27134163 |
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author | Xu, Jiahui Hu, Fengyue Li, Shuang Bao, Jiaojiao Yin, Yi Ren, Zhenyu Deng, Ying Tian, Fang Bao, Guangyu Liu, Jian Li, Yinyue He, Xinlong Xi, Juqun Lu, Feng |
author_facet | Xu, Jiahui Hu, Fengyue Li, Shuang Bao, Jiaojiao Yin, Yi Ren, Zhenyu Deng, Ying Tian, Fang Bao, Guangyu Liu, Jian Li, Yinyue He, Xinlong Xi, Juqun Lu, Feng |
author_sort | Xu, Jiahui |
collection | PubMed |
description | To verify the size and emergence time of new permeability pathways (NPPs) in malaria parasites, the permeability of the Plasmodium falciparum-infected erythrocytes was tested with different particle sizes of nanomaterials by flow cytometry assay. The results confirmed the permeability of the host cell membrane increases with parasite maturation for the stage-development evolution of NPPs, and especially found that a particle size of about 50 nm had higher efficiency. As a kind of the novel nanomaterials, nitrogen-doped carbon dots (NCDs) showed no toxicity, specificity binding ability to the malaria parasites, and could label live elder blood-stage P. falciparum through NPPs, indicating the potential application in cell imaging. NPPs and some nanomaterials such as NCDs deserve more attention and exploration for the elimination and prevention of malaria. |
format | Online Article Text |
id | pubmed-9267939 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92679392022-07-09 Fluorescent Nitrogen-Doped Carbon Dots for Label Live Elder Blood-Stage Plasmodium falciparum through New Permeability Pathways Xu, Jiahui Hu, Fengyue Li, Shuang Bao, Jiaojiao Yin, Yi Ren, Zhenyu Deng, Ying Tian, Fang Bao, Guangyu Liu, Jian Li, Yinyue He, Xinlong Xi, Juqun Lu, Feng Molecules Article To verify the size and emergence time of new permeability pathways (NPPs) in malaria parasites, the permeability of the Plasmodium falciparum-infected erythrocytes was tested with different particle sizes of nanomaterials by flow cytometry assay. The results confirmed the permeability of the host cell membrane increases with parasite maturation for the stage-development evolution of NPPs, and especially found that a particle size of about 50 nm had higher efficiency. As a kind of the novel nanomaterials, nitrogen-doped carbon dots (NCDs) showed no toxicity, specificity binding ability to the malaria parasites, and could label live elder blood-stage P. falciparum through NPPs, indicating the potential application in cell imaging. NPPs and some nanomaterials such as NCDs deserve more attention and exploration for the elimination and prevention of malaria. MDPI 2022-06-29 /pmc/articles/PMC9267939/ /pubmed/35807422 http://dx.doi.org/10.3390/molecules27134163 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xu, Jiahui Hu, Fengyue Li, Shuang Bao, Jiaojiao Yin, Yi Ren, Zhenyu Deng, Ying Tian, Fang Bao, Guangyu Liu, Jian Li, Yinyue He, Xinlong Xi, Juqun Lu, Feng Fluorescent Nitrogen-Doped Carbon Dots for Label Live Elder Blood-Stage Plasmodium falciparum through New Permeability Pathways |
title | Fluorescent Nitrogen-Doped Carbon Dots for Label Live Elder Blood-Stage Plasmodium falciparum through New Permeability Pathways |
title_full | Fluorescent Nitrogen-Doped Carbon Dots for Label Live Elder Blood-Stage Plasmodium falciparum through New Permeability Pathways |
title_fullStr | Fluorescent Nitrogen-Doped Carbon Dots for Label Live Elder Blood-Stage Plasmodium falciparum through New Permeability Pathways |
title_full_unstemmed | Fluorescent Nitrogen-Doped Carbon Dots for Label Live Elder Blood-Stage Plasmodium falciparum through New Permeability Pathways |
title_short | Fluorescent Nitrogen-Doped Carbon Dots for Label Live Elder Blood-Stage Plasmodium falciparum through New Permeability Pathways |
title_sort | fluorescent nitrogen-doped carbon dots for label live elder blood-stage plasmodium falciparum through new permeability pathways |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9267939/ https://www.ncbi.nlm.nih.gov/pubmed/35807422 http://dx.doi.org/10.3390/molecules27134163 |
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