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Stimuli-Responsive Thiomorpholine Oxide-Derived Polymers with Tailored Hydrophilicity and Hemocompatible Properties

Thermo-responsive hydrophilic polymers, including those showing tuneable lower critical solution temperature (LCST), represent a continuous subject of exploration for a variety of applications, but particularly in nanomedicine. Since biological pH changes can inform the organism about the presence o...

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Autores principales: Arsenie, Laura Vasilica, Hausig, Franziska, Kellner, Carolin, Brendel, Johannes C., Lacroix-Desmazes, Patrick, Ladmiral, Vincent, Catrouillet, Sylvain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268026/
https://www.ncbi.nlm.nih.gov/pubmed/35807477
http://dx.doi.org/10.3390/molecules27134233
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author Arsenie, Laura Vasilica
Hausig, Franziska
Kellner, Carolin
Brendel, Johannes C.
Lacroix-Desmazes, Patrick
Ladmiral, Vincent
Catrouillet, Sylvain
author_facet Arsenie, Laura Vasilica
Hausig, Franziska
Kellner, Carolin
Brendel, Johannes C.
Lacroix-Desmazes, Patrick
Ladmiral, Vincent
Catrouillet, Sylvain
author_sort Arsenie, Laura Vasilica
collection PubMed
description Thermo-responsive hydrophilic polymers, including those showing tuneable lower critical solution temperature (LCST), represent a continuous subject of exploration for a variety of applications, but particularly in nanomedicine. Since biological pH changes can inform the organism about the presence of disequilibrium or diseases, the development of dual LCST/pH-responsive hydrophilic polymers with biological potential is an attractive subject in polymer science. Here, we present a novel polymer featuring LCST/pH double responsiveness. The monomer ethylthiomorpholine oxide methacrylate (THOXMA) can be polymerised via the RAFT process to obtain well-defined polymers. Copolymers with hydroxyethyl methacrylate (HEMA) were prepared, which allowed the tuning of the LCST behaviour of the polymers. Both, the LCST behaviour and pH responsiveness of hydrophilic PTHOXMA were tested by following the evolution of particle size by dynamic light scattering (DLS). In weak and strong alkaline conditions, cloud points ranged between 40–60 °C, while in acidic medium no LCST was found due to the protonation of the amine of the THOX moieties. Additional cytotoxicity assays confirmed a high biocompatibility of PTHOXMA and haemolysis and aggregation assays proved that the thiomorpholine oxide-derived polymers did not cause aggregation or lysis of red blood cells. These preliminary results bode well for the use of PTHOXMA as smart material in biological applications.
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spelling pubmed-92680262022-07-09 Stimuli-Responsive Thiomorpholine Oxide-Derived Polymers with Tailored Hydrophilicity and Hemocompatible Properties Arsenie, Laura Vasilica Hausig, Franziska Kellner, Carolin Brendel, Johannes C. Lacroix-Desmazes, Patrick Ladmiral, Vincent Catrouillet, Sylvain Molecules Article Thermo-responsive hydrophilic polymers, including those showing tuneable lower critical solution temperature (LCST), represent a continuous subject of exploration for a variety of applications, but particularly in nanomedicine. Since biological pH changes can inform the organism about the presence of disequilibrium or diseases, the development of dual LCST/pH-responsive hydrophilic polymers with biological potential is an attractive subject in polymer science. Here, we present a novel polymer featuring LCST/pH double responsiveness. The monomer ethylthiomorpholine oxide methacrylate (THOXMA) can be polymerised via the RAFT process to obtain well-defined polymers. Copolymers with hydroxyethyl methacrylate (HEMA) were prepared, which allowed the tuning of the LCST behaviour of the polymers. Both, the LCST behaviour and pH responsiveness of hydrophilic PTHOXMA were tested by following the evolution of particle size by dynamic light scattering (DLS). In weak and strong alkaline conditions, cloud points ranged between 40–60 °C, while in acidic medium no LCST was found due to the protonation of the amine of the THOX moieties. Additional cytotoxicity assays confirmed a high biocompatibility of PTHOXMA and haemolysis and aggregation assays proved that the thiomorpholine oxide-derived polymers did not cause aggregation or lysis of red blood cells. These preliminary results bode well for the use of PTHOXMA as smart material in biological applications. MDPI 2022-06-30 /pmc/articles/PMC9268026/ /pubmed/35807477 http://dx.doi.org/10.3390/molecules27134233 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Arsenie, Laura Vasilica
Hausig, Franziska
Kellner, Carolin
Brendel, Johannes C.
Lacroix-Desmazes, Patrick
Ladmiral, Vincent
Catrouillet, Sylvain
Stimuli-Responsive Thiomorpholine Oxide-Derived Polymers with Tailored Hydrophilicity and Hemocompatible Properties
title Stimuli-Responsive Thiomorpholine Oxide-Derived Polymers with Tailored Hydrophilicity and Hemocompatible Properties
title_full Stimuli-Responsive Thiomorpholine Oxide-Derived Polymers with Tailored Hydrophilicity and Hemocompatible Properties
title_fullStr Stimuli-Responsive Thiomorpholine Oxide-Derived Polymers with Tailored Hydrophilicity and Hemocompatible Properties
title_full_unstemmed Stimuli-Responsive Thiomorpholine Oxide-Derived Polymers with Tailored Hydrophilicity and Hemocompatible Properties
title_short Stimuli-Responsive Thiomorpholine Oxide-Derived Polymers with Tailored Hydrophilicity and Hemocompatible Properties
title_sort stimuli-responsive thiomorpholine oxide-derived polymers with tailored hydrophilicity and hemocompatible properties
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268026/
https://www.ncbi.nlm.nih.gov/pubmed/35807477
http://dx.doi.org/10.3390/molecules27134233
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