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SARS-CoV-2 variants of concern partially escape humoral but not T cell responses in COVID-19 convalescent donors and vaccine recipients
The emergence of SARS-CoV-2 variants harboring mutations in the spike (S) protein has raised concern about potential immune escape. Here, we studied humoral and cellular immune responses to wild-type SARS-CoV-2 and the B.1.1.7 and B.1.351 variants of concern in a cohort of 121 BNT162b2 messenger RNA...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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American Association for the Advancement of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268159/ https://www.ncbi.nlm.nih.gov/pubmed/34035118 http://dx.doi.org/10.1126/sciimmunol.abj1750 |
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author | Geers, Daryl Shamier, Marc C. Bogers, Susanne den Hartog, Gerco Gommers, Lennert Nieuwkoop, Nella N. Schmitz, Katharina S. Rijsbergen, Laurine C. van Osch, Jolieke A. T. Dijkhuizen, Emma Smits, Gaby Comvalius, Anouskha van Mourik, Djenolan Caniels, Tom G. van Gils, Marit J. Sanders, Rogier W. Oude Munnink, Bas B. Molenkamp, Richard de Jager, Herbert J. Haagmans, Bart L. de Swart, Rik L. Koopmans, Marion P. G. van Binnendijk, Robert S. de Vries, Rory D. GeurtsvanKessel, Corine H. |
author_facet | Geers, Daryl Shamier, Marc C. Bogers, Susanne den Hartog, Gerco Gommers, Lennert Nieuwkoop, Nella N. Schmitz, Katharina S. Rijsbergen, Laurine C. van Osch, Jolieke A. T. Dijkhuizen, Emma Smits, Gaby Comvalius, Anouskha van Mourik, Djenolan Caniels, Tom G. van Gils, Marit J. Sanders, Rogier W. Oude Munnink, Bas B. Molenkamp, Richard de Jager, Herbert J. Haagmans, Bart L. de Swart, Rik L. Koopmans, Marion P. G. van Binnendijk, Robert S. de Vries, Rory D. GeurtsvanKessel, Corine H. |
author_sort | Geers, Daryl |
collection | PubMed |
description | The emergence of SARS-CoV-2 variants harboring mutations in the spike (S) protein has raised concern about potential immune escape. Here, we studied humoral and cellular immune responses to wild-type SARS-CoV-2 and the B.1.1.7 and B.1.351 variants of concern in a cohort of 121 BNT162b2 messenger RNA–vaccinated health care workers (HCWs). Twenty-three HCWs recovered from mild COVID-19 disease and exhibited a recall response with high levels of SARS-CoV-2–specific functional antibodies and virus-specific T cells after a single vaccination. Specific immune responses were also detected in seronegative HCWs after one vaccination, but a second dose was required to reach high levels of functional antibodies and cellular immune responses in all individuals. Vaccination-induced antibodies cross-neutralized the variants B.1.1.7 and B.1.351, but the neutralizing capacity and Fc-mediated functionality against B.1.351 were consistently two- to fourfold lower than those against the homologous virus. In addition, peripheral blood mononuclear cells were stimulated with peptide pools spanning the mutated S regions of B.1.1.7 and B.1.351 to detect cross-reactivity of SARS-CoV-2–specific T cells with variants. We observed no differences in CD4(+) T cell activation in response to variant antigens, indicating that the B.1.1.7 and B.1.351 S proteins do not escape T cell–mediated immunity elicited by the wild-type S protein. In conclusion, this study shows that some variants can partially escape humoral immunity induced by SARS-CoV-2 infection or BNT162b2 vaccination, but S-specific CD4(+) T cell activation is not affected by the mutations in the B.1.1.7 and B.1.351 variants. |
format | Online Article Text |
id | pubmed-9268159 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-92681592022-07-14 SARS-CoV-2 variants of concern partially escape humoral but not T cell responses in COVID-19 convalescent donors and vaccine recipients Geers, Daryl Shamier, Marc C. Bogers, Susanne den Hartog, Gerco Gommers, Lennert Nieuwkoop, Nella N. Schmitz, Katharina S. Rijsbergen, Laurine C. van Osch, Jolieke A. T. Dijkhuizen, Emma Smits, Gaby Comvalius, Anouskha van Mourik, Djenolan Caniels, Tom G. van Gils, Marit J. Sanders, Rogier W. Oude Munnink, Bas B. Molenkamp, Richard de Jager, Herbert J. Haagmans, Bart L. de Swart, Rik L. Koopmans, Marion P. G. van Binnendijk, Robert S. de Vries, Rory D. GeurtsvanKessel, Corine H. Sci Immunol Research Articles The emergence of SARS-CoV-2 variants harboring mutations in the spike (S) protein has raised concern about potential immune escape. Here, we studied humoral and cellular immune responses to wild-type SARS-CoV-2 and the B.1.1.7 and B.1.351 variants of concern in a cohort of 121 BNT162b2 messenger RNA–vaccinated health care workers (HCWs). Twenty-three HCWs recovered from mild COVID-19 disease and exhibited a recall response with high levels of SARS-CoV-2–specific functional antibodies and virus-specific T cells after a single vaccination. Specific immune responses were also detected in seronegative HCWs after one vaccination, but a second dose was required to reach high levels of functional antibodies and cellular immune responses in all individuals. Vaccination-induced antibodies cross-neutralized the variants B.1.1.7 and B.1.351, but the neutralizing capacity and Fc-mediated functionality against B.1.351 were consistently two- to fourfold lower than those against the homologous virus. In addition, peripheral blood mononuclear cells were stimulated with peptide pools spanning the mutated S regions of B.1.1.7 and B.1.351 to detect cross-reactivity of SARS-CoV-2–specific T cells with variants. We observed no differences in CD4(+) T cell activation in response to variant antigens, indicating that the B.1.1.7 and B.1.351 S proteins do not escape T cell–mediated immunity elicited by the wild-type S protein. In conclusion, this study shows that some variants can partially escape humoral immunity induced by SARS-CoV-2 infection or BNT162b2 vaccination, but S-specific CD4(+) T cell activation is not affected by the mutations in the B.1.1.7 and B.1.351 variants. American Association for the Advancement of Science 2021-05-25 /pmc/articles/PMC9268159/ /pubmed/34035118 http://dx.doi.org/10.1126/sciimmunol.abj1750 Text en Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Geers, Daryl Shamier, Marc C. Bogers, Susanne den Hartog, Gerco Gommers, Lennert Nieuwkoop, Nella N. Schmitz, Katharina S. Rijsbergen, Laurine C. van Osch, Jolieke A. T. Dijkhuizen, Emma Smits, Gaby Comvalius, Anouskha van Mourik, Djenolan Caniels, Tom G. van Gils, Marit J. Sanders, Rogier W. Oude Munnink, Bas B. Molenkamp, Richard de Jager, Herbert J. Haagmans, Bart L. de Swart, Rik L. Koopmans, Marion P. G. van Binnendijk, Robert S. de Vries, Rory D. GeurtsvanKessel, Corine H. SARS-CoV-2 variants of concern partially escape humoral but not T cell responses in COVID-19 convalescent donors and vaccine recipients |
title | SARS-CoV-2 variants of concern partially escape humoral but not T cell responses in COVID-19 convalescent donors and vaccine recipients |
title_full | SARS-CoV-2 variants of concern partially escape humoral but not T cell responses in COVID-19 convalescent donors and vaccine recipients |
title_fullStr | SARS-CoV-2 variants of concern partially escape humoral but not T cell responses in COVID-19 convalescent donors and vaccine recipients |
title_full_unstemmed | SARS-CoV-2 variants of concern partially escape humoral but not T cell responses in COVID-19 convalescent donors and vaccine recipients |
title_short | SARS-CoV-2 variants of concern partially escape humoral but not T cell responses in COVID-19 convalescent donors and vaccine recipients |
title_sort | sars-cov-2 variants of concern partially escape humoral but not t cell responses in covid-19 convalescent donors and vaccine recipients |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268159/ https://www.ncbi.nlm.nih.gov/pubmed/34035118 http://dx.doi.org/10.1126/sciimmunol.abj1750 |
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