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Cornelian Cherry (Cornus mas L.) Extracts Exert Cytotoxicity in Two Selected Melanoma Cell Lines—A Factorial Analysis of Time-Dependent Alterations in Values Obtained with SRB and MTT Assays

Despite the fact that phytochemicals of Cornaceae species have long been discussed as possible auxiliary agents in contemporary treatment, the insights on their properties remain relatively scarce. This study focuses on Cornus mas L. (Cornelian cherry), the extracts of which are reported to exert a...

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Autores principales: Lewandowski, Łukasz, Bednarz-Misa, Iwona, Kucharska, Alicja Z., Kubiak, Agnieszka, Kasprzyk, Patrycja, Sozański, Tomasz, Przybylska, Dominika, Piórecki, Narcyz, Krzystek-Korpacka, Małgorzata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268180/
https://www.ncbi.nlm.nih.gov/pubmed/35807436
http://dx.doi.org/10.3390/molecules27134193
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author Lewandowski, Łukasz
Bednarz-Misa, Iwona
Kucharska, Alicja Z.
Kubiak, Agnieszka
Kasprzyk, Patrycja
Sozański, Tomasz
Przybylska, Dominika
Piórecki, Narcyz
Krzystek-Korpacka, Małgorzata
author_facet Lewandowski, Łukasz
Bednarz-Misa, Iwona
Kucharska, Alicja Z.
Kubiak, Agnieszka
Kasprzyk, Patrycja
Sozański, Tomasz
Przybylska, Dominika
Piórecki, Narcyz
Krzystek-Korpacka, Małgorzata
author_sort Lewandowski, Łukasz
collection PubMed
description Despite the fact that phytochemicals of Cornaceae species have long been discussed as possible auxiliary agents in contemporary treatment, the insights on their properties remain relatively scarce. This study focuses on Cornus mas L. (Cornelian cherry), the extracts of which are reported to exert a pleiotropic effect shown in both in vivo and in vitro studies. This study aimed to explore the cytotoxic effect of extracts from fruits of red (Cornus mas L. ‘Podolski’) and yellow (Cornus mas L. ‘Yantarnyi’ and ‘Flava’) Cornelian cherries on two melanoma cell lines (A375 and MeWo). The extracts were characterized in the context of the concentration of bioactive compounds of antioxidative properties. Cytotoxicity was investigated with the use of the following two assays: SRB and MTT. An additional, alternative protocol for the SRB assay was used in this study so as to account for possible bias. Cytotoxicity was assessed as a difference in the whole time series of cell viability, instead of analyzing differences in raw values (often found in the literature). Both extracts from Cornus mas L. induced cytotoxicity in both A375 and MeWo cell lines, although the response of these cells was different. Moreover, based on this study, there is no evidence for claiming a different magnitude of cytotoxicity between these two extracts.
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spelling pubmed-92681802022-07-09 Cornelian Cherry (Cornus mas L.) Extracts Exert Cytotoxicity in Two Selected Melanoma Cell Lines—A Factorial Analysis of Time-Dependent Alterations in Values Obtained with SRB and MTT Assays Lewandowski, Łukasz Bednarz-Misa, Iwona Kucharska, Alicja Z. Kubiak, Agnieszka Kasprzyk, Patrycja Sozański, Tomasz Przybylska, Dominika Piórecki, Narcyz Krzystek-Korpacka, Małgorzata Molecules Article Despite the fact that phytochemicals of Cornaceae species have long been discussed as possible auxiliary agents in contemporary treatment, the insights on their properties remain relatively scarce. This study focuses on Cornus mas L. (Cornelian cherry), the extracts of which are reported to exert a pleiotropic effect shown in both in vivo and in vitro studies. This study aimed to explore the cytotoxic effect of extracts from fruits of red (Cornus mas L. ‘Podolski’) and yellow (Cornus mas L. ‘Yantarnyi’ and ‘Flava’) Cornelian cherries on two melanoma cell lines (A375 and MeWo). The extracts were characterized in the context of the concentration of bioactive compounds of antioxidative properties. Cytotoxicity was investigated with the use of the following two assays: SRB and MTT. An additional, alternative protocol for the SRB assay was used in this study so as to account for possible bias. Cytotoxicity was assessed as a difference in the whole time series of cell viability, instead of analyzing differences in raw values (often found in the literature). Both extracts from Cornus mas L. induced cytotoxicity in both A375 and MeWo cell lines, although the response of these cells was different. Moreover, based on this study, there is no evidence for claiming a different magnitude of cytotoxicity between these two extracts. MDPI 2022-06-29 /pmc/articles/PMC9268180/ /pubmed/35807436 http://dx.doi.org/10.3390/molecules27134193 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lewandowski, Łukasz
Bednarz-Misa, Iwona
Kucharska, Alicja Z.
Kubiak, Agnieszka
Kasprzyk, Patrycja
Sozański, Tomasz
Przybylska, Dominika
Piórecki, Narcyz
Krzystek-Korpacka, Małgorzata
Cornelian Cherry (Cornus mas L.) Extracts Exert Cytotoxicity in Two Selected Melanoma Cell Lines—A Factorial Analysis of Time-Dependent Alterations in Values Obtained with SRB and MTT Assays
title Cornelian Cherry (Cornus mas L.) Extracts Exert Cytotoxicity in Two Selected Melanoma Cell Lines—A Factorial Analysis of Time-Dependent Alterations in Values Obtained with SRB and MTT Assays
title_full Cornelian Cherry (Cornus mas L.) Extracts Exert Cytotoxicity in Two Selected Melanoma Cell Lines—A Factorial Analysis of Time-Dependent Alterations in Values Obtained with SRB and MTT Assays
title_fullStr Cornelian Cherry (Cornus mas L.) Extracts Exert Cytotoxicity in Two Selected Melanoma Cell Lines—A Factorial Analysis of Time-Dependent Alterations in Values Obtained with SRB and MTT Assays
title_full_unstemmed Cornelian Cherry (Cornus mas L.) Extracts Exert Cytotoxicity in Two Selected Melanoma Cell Lines—A Factorial Analysis of Time-Dependent Alterations in Values Obtained with SRB and MTT Assays
title_short Cornelian Cherry (Cornus mas L.) Extracts Exert Cytotoxicity in Two Selected Melanoma Cell Lines—A Factorial Analysis of Time-Dependent Alterations in Values Obtained with SRB and MTT Assays
title_sort cornelian cherry (cornus mas l.) extracts exert cytotoxicity in two selected melanoma cell lines—a factorial analysis of time-dependent alterations in values obtained with srb and mtt assays
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268180/
https://www.ncbi.nlm.nih.gov/pubmed/35807436
http://dx.doi.org/10.3390/molecules27134193
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