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Adlay Seed (Coix lacryma-jobi L. var. Ma-yuen Stapf.) Ethanolic Extract Fractions and Subfractions Induce Cell Cycle Arrest and Apoptosis in Human Breast and Cervical Cancer Cell Lines

The antitumor effects of Coix lacryma-jobi L. var. ma-yuen Stapf. (adlay seed) ethanolic extract have been increasingly shown. This study aimed to investigate the beneficial effects of both the fractions and subfractions of adlay seed ethanolic extract on the human breast (MCF-7) and cervical (HeLa)...

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Autores principales: Chiang, Yi-Fen, Chung, Cheng-Pei, Lin, Jing-Hui, Chiang, Wenchang, Chen, Hsin-Yuan, Ali, Mohamed, Shih, Yin-Hwa, Wang, Kai-Lee, Huang, Tsui-Chin, Chang, Hsin-Yi, Lin, Li-Chun, Shieh, Tzong-Ming, Hsia, Shih-Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268182/
https://www.ncbi.nlm.nih.gov/pubmed/35807238
http://dx.doi.org/10.3390/molecules27133984
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author Chiang, Yi-Fen
Chung, Cheng-Pei
Lin, Jing-Hui
Chiang, Wenchang
Chen, Hsin-Yuan
Ali, Mohamed
Shih, Yin-Hwa
Wang, Kai-Lee
Huang, Tsui-Chin
Chang, Hsin-Yi
Lin, Li-Chun
Shieh, Tzong-Ming
Hsia, Shih-Min
author_facet Chiang, Yi-Fen
Chung, Cheng-Pei
Lin, Jing-Hui
Chiang, Wenchang
Chen, Hsin-Yuan
Ali, Mohamed
Shih, Yin-Hwa
Wang, Kai-Lee
Huang, Tsui-Chin
Chang, Hsin-Yi
Lin, Li-Chun
Shieh, Tzong-Ming
Hsia, Shih-Min
author_sort Chiang, Yi-Fen
collection PubMed
description The antitumor effects of Coix lacryma-jobi L. var. ma-yuen Stapf. (adlay seed) ethanolic extract have been increasingly shown. This study aimed to investigate the beneficial effects of both the fractions and subfractions of adlay seed ethanolic extract on the human breast (MCF-7) and cervical (HeLa) cancer cell lines, as well as exploring their possible mechanisms of action. The ethanolic extracts were obtained from different parts of adlay seed, including AHE (adlay hull extract), ATE (adlay testa extract), ABE (adlay bran extract) and PAE (polished adlay extract). The results of a 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl- tetrazolium bromide (MTT) assay showed that AHE-Ea and ATE-Ea showed significant growth inhibitory effects in a dose-dependent manner. The results also showed that the AHE-Ea-K, AHE-Ea-L, ATE-Ea-E and ATE-Ea-F subfractions inhibited cell proliferation, induced cell cycle arrest in the G0/G1 phase and decreased CDK4/Cyclin D1 protein expression. Finally, the extract activated caspase-3 activity and PARP protein expression, which induced MCF-7 and HeLa cell apoptosis. We then used liquid chromatography–mass spectrometry (LC/MS) to identify the potential active components., Quercetin showed an anticancer capacity. In conclusion, the AHE-Ea-K, AHE-Ea-L, ATE-Ea-E and ATE-Ea-F subfractions showed antitumor effects through the inhibition of MCF-7 and HeLa cell line viability, as well as inducing apoptosis and cell cycle arrest.
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spelling pubmed-92681822022-07-09 Adlay Seed (Coix lacryma-jobi L. var. Ma-yuen Stapf.) Ethanolic Extract Fractions and Subfractions Induce Cell Cycle Arrest and Apoptosis in Human Breast and Cervical Cancer Cell Lines Chiang, Yi-Fen Chung, Cheng-Pei Lin, Jing-Hui Chiang, Wenchang Chen, Hsin-Yuan Ali, Mohamed Shih, Yin-Hwa Wang, Kai-Lee Huang, Tsui-Chin Chang, Hsin-Yi Lin, Li-Chun Shieh, Tzong-Ming Hsia, Shih-Min Molecules Article The antitumor effects of Coix lacryma-jobi L. var. ma-yuen Stapf. (adlay seed) ethanolic extract have been increasingly shown. This study aimed to investigate the beneficial effects of both the fractions and subfractions of adlay seed ethanolic extract on the human breast (MCF-7) and cervical (HeLa) cancer cell lines, as well as exploring their possible mechanisms of action. The ethanolic extracts were obtained from different parts of adlay seed, including AHE (adlay hull extract), ATE (adlay testa extract), ABE (adlay bran extract) and PAE (polished adlay extract). The results of a 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl- tetrazolium bromide (MTT) assay showed that AHE-Ea and ATE-Ea showed significant growth inhibitory effects in a dose-dependent manner. The results also showed that the AHE-Ea-K, AHE-Ea-L, ATE-Ea-E and ATE-Ea-F subfractions inhibited cell proliferation, induced cell cycle arrest in the G0/G1 phase and decreased CDK4/Cyclin D1 protein expression. Finally, the extract activated caspase-3 activity and PARP protein expression, which induced MCF-7 and HeLa cell apoptosis. We then used liquid chromatography–mass spectrometry (LC/MS) to identify the potential active components., Quercetin showed an anticancer capacity. In conclusion, the AHE-Ea-K, AHE-Ea-L, ATE-Ea-E and ATE-Ea-F subfractions showed antitumor effects through the inhibition of MCF-7 and HeLa cell line viability, as well as inducing apoptosis and cell cycle arrest. MDPI 2022-06-21 /pmc/articles/PMC9268182/ /pubmed/35807238 http://dx.doi.org/10.3390/molecules27133984 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chiang, Yi-Fen
Chung, Cheng-Pei
Lin, Jing-Hui
Chiang, Wenchang
Chen, Hsin-Yuan
Ali, Mohamed
Shih, Yin-Hwa
Wang, Kai-Lee
Huang, Tsui-Chin
Chang, Hsin-Yi
Lin, Li-Chun
Shieh, Tzong-Ming
Hsia, Shih-Min
Adlay Seed (Coix lacryma-jobi L. var. Ma-yuen Stapf.) Ethanolic Extract Fractions and Subfractions Induce Cell Cycle Arrest and Apoptosis in Human Breast and Cervical Cancer Cell Lines
title Adlay Seed (Coix lacryma-jobi L. var. Ma-yuen Stapf.) Ethanolic Extract Fractions and Subfractions Induce Cell Cycle Arrest and Apoptosis in Human Breast and Cervical Cancer Cell Lines
title_full Adlay Seed (Coix lacryma-jobi L. var. Ma-yuen Stapf.) Ethanolic Extract Fractions and Subfractions Induce Cell Cycle Arrest and Apoptosis in Human Breast and Cervical Cancer Cell Lines
title_fullStr Adlay Seed (Coix lacryma-jobi L. var. Ma-yuen Stapf.) Ethanolic Extract Fractions and Subfractions Induce Cell Cycle Arrest and Apoptosis in Human Breast and Cervical Cancer Cell Lines
title_full_unstemmed Adlay Seed (Coix lacryma-jobi L. var. Ma-yuen Stapf.) Ethanolic Extract Fractions and Subfractions Induce Cell Cycle Arrest and Apoptosis in Human Breast and Cervical Cancer Cell Lines
title_short Adlay Seed (Coix lacryma-jobi L. var. Ma-yuen Stapf.) Ethanolic Extract Fractions and Subfractions Induce Cell Cycle Arrest and Apoptosis in Human Breast and Cervical Cancer Cell Lines
title_sort adlay seed (coix lacryma-jobi l. var. ma-yuen stapf.) ethanolic extract fractions and subfractions induce cell cycle arrest and apoptosis in human breast and cervical cancer cell lines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268182/
https://www.ncbi.nlm.nih.gov/pubmed/35807238
http://dx.doi.org/10.3390/molecules27133984
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