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Myocardial Injury Caused by Chronic Alcohol Exposure—A Pilot Study Based on Proteomics

Chronic alcohol exposure can cause myocardial degenerative diseases, manifested as cardiac insufficiency, arrhythmia, etc. These are defined as alcoholic cardiomyopathy (ACM). Alcohol-mediated myocardial injury has previously been studied through metabolomics, and it has been proved to be involved i...

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Autores principales: Ma, Xiaonan, Liao, Zihan, Li, Rongxuan, Xia, Wei, Guo, Honghui, Luo, Jiawei, Sheng, Huaxin, Tian, Meihui, Cao, Zhipeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268295/
https://www.ncbi.nlm.nih.gov/pubmed/35807529
http://dx.doi.org/10.3390/molecules27134284
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author Ma, Xiaonan
Liao, Zihan
Li, Rongxuan
Xia, Wei
Guo, Honghui
Luo, Jiawei
Sheng, Huaxin
Tian, Meihui
Cao, Zhipeng
author_facet Ma, Xiaonan
Liao, Zihan
Li, Rongxuan
Xia, Wei
Guo, Honghui
Luo, Jiawei
Sheng, Huaxin
Tian, Meihui
Cao, Zhipeng
author_sort Ma, Xiaonan
collection PubMed
description Chronic alcohol exposure can cause myocardial degenerative diseases, manifested as cardiac insufficiency, arrhythmia, etc. These are defined as alcoholic cardiomyopathy (ACM). Alcohol-mediated myocardial injury has previously been studied through metabolomics, and it has been proved to be involved in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway concerning unsaturated fatty acids biosynthesis and oxidative phosphorylation, which tentatively explored the mechanism of ACM induced by chronic drinking. To further study alcohol-induced myocardial injury, myocardial specimens from a previously successfully established mouse model of ACM were subjected to histological, echocardiographic, and proteomic analyses, and validated by real-time quantitative polymerase chain reaction (qPCR). Results of histopathology and echocardiography showed the hypertrophy of cardiomyocytes, the dilation of ventricles, and decreased cardiac function. Proteomic results, available via ProteomeXchange with identifier PXD032949, revealed 56 differentially expressed proteins (DEPs) were identified, which have the potential to be involved in the KEGG pathway related to fatty acid biosynthesis disorders, lipid metabolism disorders, oxidative stress, and, ultimately, in the development of dilated cardiomyopathy (DCM). The present study further elucidates the underlying effects of myocardial injury due to chronic alcohol intake, laying a foundation for further studies to clarify the potential mechanisms of ACM.
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spelling pubmed-92682952022-07-09 Myocardial Injury Caused by Chronic Alcohol Exposure—A Pilot Study Based on Proteomics Ma, Xiaonan Liao, Zihan Li, Rongxuan Xia, Wei Guo, Honghui Luo, Jiawei Sheng, Huaxin Tian, Meihui Cao, Zhipeng Molecules Article Chronic alcohol exposure can cause myocardial degenerative diseases, manifested as cardiac insufficiency, arrhythmia, etc. These are defined as alcoholic cardiomyopathy (ACM). Alcohol-mediated myocardial injury has previously been studied through metabolomics, and it has been proved to be involved in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway concerning unsaturated fatty acids biosynthesis and oxidative phosphorylation, which tentatively explored the mechanism of ACM induced by chronic drinking. To further study alcohol-induced myocardial injury, myocardial specimens from a previously successfully established mouse model of ACM were subjected to histological, echocardiographic, and proteomic analyses, and validated by real-time quantitative polymerase chain reaction (qPCR). Results of histopathology and echocardiography showed the hypertrophy of cardiomyocytes, the dilation of ventricles, and decreased cardiac function. Proteomic results, available via ProteomeXchange with identifier PXD032949, revealed 56 differentially expressed proteins (DEPs) were identified, which have the potential to be involved in the KEGG pathway related to fatty acid biosynthesis disorders, lipid metabolism disorders, oxidative stress, and, ultimately, in the development of dilated cardiomyopathy (DCM). The present study further elucidates the underlying effects of myocardial injury due to chronic alcohol intake, laying a foundation for further studies to clarify the potential mechanisms of ACM. MDPI 2022-07-03 /pmc/articles/PMC9268295/ /pubmed/35807529 http://dx.doi.org/10.3390/molecules27134284 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ma, Xiaonan
Liao, Zihan
Li, Rongxuan
Xia, Wei
Guo, Honghui
Luo, Jiawei
Sheng, Huaxin
Tian, Meihui
Cao, Zhipeng
Myocardial Injury Caused by Chronic Alcohol Exposure—A Pilot Study Based on Proteomics
title Myocardial Injury Caused by Chronic Alcohol Exposure—A Pilot Study Based on Proteomics
title_full Myocardial Injury Caused by Chronic Alcohol Exposure—A Pilot Study Based on Proteomics
title_fullStr Myocardial Injury Caused by Chronic Alcohol Exposure—A Pilot Study Based on Proteomics
title_full_unstemmed Myocardial Injury Caused by Chronic Alcohol Exposure—A Pilot Study Based on Proteomics
title_short Myocardial Injury Caused by Chronic Alcohol Exposure—A Pilot Study Based on Proteomics
title_sort myocardial injury caused by chronic alcohol exposure—a pilot study based on proteomics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268295/
https://www.ncbi.nlm.nih.gov/pubmed/35807529
http://dx.doi.org/10.3390/molecules27134284
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