Cargando…
Myocardial Injury Caused by Chronic Alcohol Exposure—A Pilot Study Based on Proteomics
Chronic alcohol exposure can cause myocardial degenerative diseases, manifested as cardiac insufficiency, arrhythmia, etc. These are defined as alcoholic cardiomyopathy (ACM). Alcohol-mediated myocardial injury has previously been studied through metabolomics, and it has been proved to be involved i...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268295/ https://www.ncbi.nlm.nih.gov/pubmed/35807529 http://dx.doi.org/10.3390/molecules27134284 |
_version_ | 1784743943163871232 |
---|---|
author | Ma, Xiaonan Liao, Zihan Li, Rongxuan Xia, Wei Guo, Honghui Luo, Jiawei Sheng, Huaxin Tian, Meihui Cao, Zhipeng |
author_facet | Ma, Xiaonan Liao, Zihan Li, Rongxuan Xia, Wei Guo, Honghui Luo, Jiawei Sheng, Huaxin Tian, Meihui Cao, Zhipeng |
author_sort | Ma, Xiaonan |
collection | PubMed |
description | Chronic alcohol exposure can cause myocardial degenerative diseases, manifested as cardiac insufficiency, arrhythmia, etc. These are defined as alcoholic cardiomyopathy (ACM). Alcohol-mediated myocardial injury has previously been studied through metabolomics, and it has been proved to be involved in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway concerning unsaturated fatty acids biosynthesis and oxidative phosphorylation, which tentatively explored the mechanism of ACM induced by chronic drinking. To further study alcohol-induced myocardial injury, myocardial specimens from a previously successfully established mouse model of ACM were subjected to histological, echocardiographic, and proteomic analyses, and validated by real-time quantitative polymerase chain reaction (qPCR). Results of histopathology and echocardiography showed the hypertrophy of cardiomyocytes, the dilation of ventricles, and decreased cardiac function. Proteomic results, available via ProteomeXchange with identifier PXD032949, revealed 56 differentially expressed proteins (DEPs) were identified, which have the potential to be involved in the KEGG pathway related to fatty acid biosynthesis disorders, lipid metabolism disorders, oxidative stress, and, ultimately, in the development of dilated cardiomyopathy (DCM). The present study further elucidates the underlying effects of myocardial injury due to chronic alcohol intake, laying a foundation for further studies to clarify the potential mechanisms of ACM. |
format | Online Article Text |
id | pubmed-9268295 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92682952022-07-09 Myocardial Injury Caused by Chronic Alcohol Exposure—A Pilot Study Based on Proteomics Ma, Xiaonan Liao, Zihan Li, Rongxuan Xia, Wei Guo, Honghui Luo, Jiawei Sheng, Huaxin Tian, Meihui Cao, Zhipeng Molecules Article Chronic alcohol exposure can cause myocardial degenerative diseases, manifested as cardiac insufficiency, arrhythmia, etc. These are defined as alcoholic cardiomyopathy (ACM). Alcohol-mediated myocardial injury has previously been studied through metabolomics, and it has been proved to be involved in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway concerning unsaturated fatty acids biosynthesis and oxidative phosphorylation, which tentatively explored the mechanism of ACM induced by chronic drinking. To further study alcohol-induced myocardial injury, myocardial specimens from a previously successfully established mouse model of ACM were subjected to histological, echocardiographic, and proteomic analyses, and validated by real-time quantitative polymerase chain reaction (qPCR). Results of histopathology and echocardiography showed the hypertrophy of cardiomyocytes, the dilation of ventricles, and decreased cardiac function. Proteomic results, available via ProteomeXchange with identifier PXD032949, revealed 56 differentially expressed proteins (DEPs) were identified, which have the potential to be involved in the KEGG pathway related to fatty acid biosynthesis disorders, lipid metabolism disorders, oxidative stress, and, ultimately, in the development of dilated cardiomyopathy (DCM). The present study further elucidates the underlying effects of myocardial injury due to chronic alcohol intake, laying a foundation for further studies to clarify the potential mechanisms of ACM. MDPI 2022-07-03 /pmc/articles/PMC9268295/ /pubmed/35807529 http://dx.doi.org/10.3390/molecules27134284 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ma, Xiaonan Liao, Zihan Li, Rongxuan Xia, Wei Guo, Honghui Luo, Jiawei Sheng, Huaxin Tian, Meihui Cao, Zhipeng Myocardial Injury Caused by Chronic Alcohol Exposure—A Pilot Study Based on Proteomics |
title | Myocardial Injury Caused by Chronic Alcohol Exposure—A Pilot Study Based on Proteomics |
title_full | Myocardial Injury Caused by Chronic Alcohol Exposure—A Pilot Study Based on Proteomics |
title_fullStr | Myocardial Injury Caused by Chronic Alcohol Exposure—A Pilot Study Based on Proteomics |
title_full_unstemmed | Myocardial Injury Caused by Chronic Alcohol Exposure—A Pilot Study Based on Proteomics |
title_short | Myocardial Injury Caused by Chronic Alcohol Exposure—A Pilot Study Based on Proteomics |
title_sort | myocardial injury caused by chronic alcohol exposure—a pilot study based on proteomics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268295/ https://www.ncbi.nlm.nih.gov/pubmed/35807529 http://dx.doi.org/10.3390/molecules27134284 |
work_keys_str_mv | AT maxiaonan myocardialinjurycausedbychronicalcoholexposureapilotstudybasedonproteomics AT liaozihan myocardialinjurycausedbychronicalcoholexposureapilotstudybasedonproteomics AT lirongxuan myocardialinjurycausedbychronicalcoholexposureapilotstudybasedonproteomics AT xiawei myocardialinjurycausedbychronicalcoholexposureapilotstudybasedonproteomics AT guohonghui myocardialinjurycausedbychronicalcoholexposureapilotstudybasedonproteomics AT luojiawei myocardialinjurycausedbychronicalcoholexposureapilotstudybasedonproteomics AT shenghuaxin myocardialinjurycausedbychronicalcoholexposureapilotstudybasedonproteomics AT tianmeihui myocardialinjurycausedbychronicalcoholexposureapilotstudybasedonproteomics AT caozhipeng myocardialinjurycausedbychronicalcoholexposureapilotstudybasedonproteomics |