The DI-SPME Method for Determination of Selected Narcotics and Their Metabolites, and Application to Bone Marrow and Whole Blood Analysis

The present investigation utilised the quick and easy SPME/LC-MS method to determine selected narcotic substances and their metabolites in whole blood. The study included qualitative analysis and validation of the method. Analytes were determined in the linearity range of 25–300 ng/mL. The precision...

Descripción completa

Detalles Bibliográficos
Autores principales: Świądro-Piętoń, Magdalena, Chromiec, Alicja, Zawadzki, Marcin, Wietecha-Posłuszny, Renata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268437/
https://www.ncbi.nlm.nih.gov/pubmed/35807361
http://dx.doi.org/10.3390/molecules27134116
_version_ 1784743982142586880
author Świądro-Piętoń, Magdalena
Chromiec, Alicja
Zawadzki, Marcin
Wietecha-Posłuszny, Renata
author_facet Świądro-Piętoń, Magdalena
Chromiec, Alicja
Zawadzki, Marcin
Wietecha-Posłuszny, Renata
author_sort Świądro-Piętoń, Magdalena
collection PubMed
description The present investigation utilised the quick and easy SPME/LC-MS method to determine selected narcotic substances and their metabolites in whole blood. The study included qualitative analysis and validation of the method. Analytes were determined in the linearity range of 25–300 ng/mL. The precision during and between days (in general CV < 13.41%), and the LOD which results in between 0.36 and 11.08 ng/mL, and the LOQ between 1.20 and 36.90 ng/mL were investigated. The validation results obtained, as well as the results of subsequent in-laboratory tests, confirmed the applicability of the method in the analysis of blood samples. An attempt to apply the method to the analysis of bone marrow samples has yielded promising results; however, more detailed studies are needed in this area.
format Online
Article
Text
id pubmed-9268437
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-92684372022-07-09 The DI-SPME Method for Determination of Selected Narcotics and Their Metabolites, and Application to Bone Marrow and Whole Blood Analysis Świądro-Piętoń, Magdalena Chromiec, Alicja Zawadzki, Marcin Wietecha-Posłuszny, Renata Molecules Article The present investigation utilised the quick and easy SPME/LC-MS method to determine selected narcotic substances and their metabolites in whole blood. The study included qualitative analysis and validation of the method. Analytes were determined in the linearity range of 25–300 ng/mL. The precision during and between days (in general CV < 13.41%), and the LOD which results in between 0.36 and 11.08 ng/mL, and the LOQ between 1.20 and 36.90 ng/mL were investigated. The validation results obtained, as well as the results of subsequent in-laboratory tests, confirmed the applicability of the method in the analysis of blood samples. An attempt to apply the method to the analysis of bone marrow samples has yielded promising results; however, more detailed studies are needed in this area. MDPI 2022-06-27 /pmc/articles/PMC9268437/ /pubmed/35807361 http://dx.doi.org/10.3390/molecules27134116 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Świądro-Piętoń, Magdalena
Chromiec, Alicja
Zawadzki, Marcin
Wietecha-Posłuszny, Renata
The DI-SPME Method for Determination of Selected Narcotics and Their Metabolites, and Application to Bone Marrow and Whole Blood Analysis
title The DI-SPME Method for Determination of Selected Narcotics and Their Metabolites, and Application to Bone Marrow and Whole Blood Analysis
title_full The DI-SPME Method for Determination of Selected Narcotics and Their Metabolites, and Application to Bone Marrow and Whole Blood Analysis
title_fullStr The DI-SPME Method for Determination of Selected Narcotics and Their Metabolites, and Application to Bone Marrow and Whole Blood Analysis
title_full_unstemmed The DI-SPME Method for Determination of Selected Narcotics and Their Metabolites, and Application to Bone Marrow and Whole Blood Analysis
title_short The DI-SPME Method for Determination of Selected Narcotics and Their Metabolites, and Application to Bone Marrow and Whole Blood Analysis
title_sort di-spme method for determination of selected narcotics and their metabolites, and application to bone marrow and whole blood analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268437/
https://www.ncbi.nlm.nih.gov/pubmed/35807361
http://dx.doi.org/10.3390/molecules27134116
work_keys_str_mv AT swiadropietonmagdalena thedispmemethodfordeterminationofselectednarcoticsandtheirmetabolitesandapplicationtobonemarrowandwholebloodanalysis
AT chromiecalicja thedispmemethodfordeterminationofselectednarcoticsandtheirmetabolitesandapplicationtobonemarrowandwholebloodanalysis
AT zawadzkimarcin thedispmemethodfordeterminationofselectednarcoticsandtheirmetabolitesandapplicationtobonemarrowandwholebloodanalysis
AT wietechaposłusznyrenata thedispmemethodfordeterminationofselectednarcoticsandtheirmetabolitesandapplicationtobonemarrowandwholebloodanalysis
AT swiadropietonmagdalena dispmemethodfordeterminationofselectednarcoticsandtheirmetabolitesandapplicationtobonemarrowandwholebloodanalysis
AT chromiecalicja dispmemethodfordeterminationofselectednarcoticsandtheirmetabolitesandapplicationtobonemarrowandwholebloodanalysis
AT zawadzkimarcin dispmemethodfordeterminationofselectednarcoticsandtheirmetabolitesandapplicationtobonemarrowandwholebloodanalysis
AT wietechaposłusznyrenata dispmemethodfordeterminationofselectednarcoticsandtheirmetabolitesandapplicationtobonemarrowandwholebloodanalysis

Ejemplares similares