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Synergistic Effect of Bioactive Monoterpenes against the Mosquito, Culex pipiens (Diptera: Culicidae)

Mosquitoes represent one of the most important vectors and are responsible for the transmission of many arboviruses that affect human and animal health. The chemical method using synthetic insecticides disturbs the environmental system and promotes the appearance of resistant insect species. Therefo...

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Autores principales: Ramzi, Amal, El Ouali Lalami, Abdelhakim, Annemer, Saoussan, Ez zoubi, Yassine, Assouguem, Amine, Almutairi, Mikhlid H., Kamel, Mohamed, Peluso, Ilaria, Ercisli, Sezai, Farah, Abdellah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268466/
https://www.ncbi.nlm.nih.gov/pubmed/35807427
http://dx.doi.org/10.3390/molecules27134182
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author Ramzi, Amal
El Ouali Lalami, Abdelhakim
Annemer, Saoussan
Ez zoubi, Yassine
Assouguem, Amine
Almutairi, Mikhlid H.
Kamel, Mohamed
Peluso, Ilaria
Ercisli, Sezai
Farah, Abdellah
author_facet Ramzi, Amal
El Ouali Lalami, Abdelhakim
Annemer, Saoussan
Ez zoubi, Yassine
Assouguem, Amine
Almutairi, Mikhlid H.
Kamel, Mohamed
Peluso, Ilaria
Ercisli, Sezai
Farah, Abdellah
author_sort Ramzi, Amal
collection PubMed
description Mosquitoes represent one of the most important vectors and are responsible for the transmission of many arboviruses that affect human and animal health. The chemical method using synthetic insecticides disturbs the environmental system and promotes the appearance of resistant insect species. Therefore, this study investigated the insecticidal effect of some binary monoterpene combinations (1,8 cineole + α-pinene and carvone + R (+)-pulegone) using a mixture design approach. The fumigant toxicity was evaluated against Culex pipiens female adults using glass jars. The results show that the toxicity varies according to the proportions of each compound. Indeed, Mixture 1 (1,8-cineole + α-pinene) displayed a strong toxic effect (51.00 ± 0.86% after 24 h and 100.00 ± 0.70% after 48 h) when the pure compounds were tested at 0.25/0.75 proportions of 1,8-cineole and α-pinene, respectively. Nevertheless, the equal proportion (0.5/0.5) of carvone and R (+)-pulegone in Mixture 2 exhibited a toxic effect of 54.35 ± 0.75% after 24 h and 89.96 ± 0.14% after 48 h, respectively. For Mixture 1, the maximum area of mortality that the proposed model indicated was obtained between 0/1 and 0.25/0.75, while the maximum area of mortality in the case of Mixture 2 was obtained between 0.25/0.75 and 0.75/0.25. Moreover, the maximum possible values of mortality that could be achieved by the validated model were found to be 51.44% (after 24 h) and 100.24% (after 48 h) for Mixture 1 and 54.67% (after 24 h) and 89.99% (after 48 h) for Mixture 2. It can be said that all purev molecules tested through the binary mixtures acted together, which enhanced the insecticide’s effectiveness. These findings are very promising, as the chemical insecticide (deltamethrin) killed only 19.29 ± 0.01% and 34.05 ± 1.01% of the female adults after 24 h and 48 h, respectively. Thus, the findings of our research could help with the development of botanical insecticides that might contribute to management programs for controlling vectors of important diseases.
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spelling pubmed-92684662022-07-09 Synergistic Effect of Bioactive Monoterpenes against the Mosquito, Culex pipiens (Diptera: Culicidae) Ramzi, Amal El Ouali Lalami, Abdelhakim Annemer, Saoussan Ez zoubi, Yassine Assouguem, Amine Almutairi, Mikhlid H. Kamel, Mohamed Peluso, Ilaria Ercisli, Sezai Farah, Abdellah Molecules Article Mosquitoes represent one of the most important vectors and are responsible for the transmission of many arboviruses that affect human and animal health. The chemical method using synthetic insecticides disturbs the environmental system and promotes the appearance of resistant insect species. Therefore, this study investigated the insecticidal effect of some binary monoterpene combinations (1,8 cineole + α-pinene and carvone + R (+)-pulegone) using a mixture design approach. The fumigant toxicity was evaluated against Culex pipiens female adults using glass jars. The results show that the toxicity varies according to the proportions of each compound. Indeed, Mixture 1 (1,8-cineole + α-pinene) displayed a strong toxic effect (51.00 ± 0.86% after 24 h and 100.00 ± 0.70% after 48 h) when the pure compounds were tested at 0.25/0.75 proportions of 1,8-cineole and α-pinene, respectively. Nevertheless, the equal proportion (0.5/0.5) of carvone and R (+)-pulegone in Mixture 2 exhibited a toxic effect of 54.35 ± 0.75% after 24 h and 89.96 ± 0.14% after 48 h, respectively. For Mixture 1, the maximum area of mortality that the proposed model indicated was obtained between 0/1 and 0.25/0.75, while the maximum area of mortality in the case of Mixture 2 was obtained between 0.25/0.75 and 0.75/0.25. Moreover, the maximum possible values of mortality that could be achieved by the validated model were found to be 51.44% (after 24 h) and 100.24% (after 48 h) for Mixture 1 and 54.67% (after 24 h) and 89.99% (after 48 h) for Mixture 2. It can be said that all purev molecules tested through the binary mixtures acted together, which enhanced the insecticide’s effectiveness. These findings are very promising, as the chemical insecticide (deltamethrin) killed only 19.29 ± 0.01% and 34.05 ± 1.01% of the female adults after 24 h and 48 h, respectively. Thus, the findings of our research could help with the development of botanical insecticides that might contribute to management programs for controlling vectors of important diseases. MDPI 2022-06-29 /pmc/articles/PMC9268466/ /pubmed/35807427 http://dx.doi.org/10.3390/molecules27134182 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ramzi, Amal
El Ouali Lalami, Abdelhakim
Annemer, Saoussan
Ez zoubi, Yassine
Assouguem, Amine
Almutairi, Mikhlid H.
Kamel, Mohamed
Peluso, Ilaria
Ercisli, Sezai
Farah, Abdellah
Synergistic Effect of Bioactive Monoterpenes against the Mosquito, Culex pipiens (Diptera: Culicidae)
title Synergistic Effect of Bioactive Monoterpenes against the Mosquito, Culex pipiens (Diptera: Culicidae)
title_full Synergistic Effect of Bioactive Monoterpenes against the Mosquito, Culex pipiens (Diptera: Culicidae)
title_fullStr Synergistic Effect of Bioactive Monoterpenes against the Mosquito, Culex pipiens (Diptera: Culicidae)
title_full_unstemmed Synergistic Effect of Bioactive Monoterpenes against the Mosquito, Culex pipiens (Diptera: Culicidae)
title_short Synergistic Effect of Bioactive Monoterpenes against the Mosquito, Culex pipiens (Diptera: Culicidae)
title_sort synergistic effect of bioactive monoterpenes against the mosquito, culex pipiens (diptera: culicidae)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268466/
https://www.ncbi.nlm.nih.gov/pubmed/35807427
http://dx.doi.org/10.3390/molecules27134182
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