Cargando…
Octanoic Acid-Enrichment Diet Improves Endurance Capacity and Reprograms Mitochondrial Biogenesis in Skeletal Muscle of Mice
Background: Medium Chain Fatty Acids (MCFAs) are a dietary supplement that exhibit interesting properties, due to their smaller molecular size. The acute consumption of MCFAs is expected to enhance exercise performance. However, the short-term effects of MCFAs on endurance performance remains poorly...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268503/ https://www.ncbi.nlm.nih.gov/pubmed/35807901 http://dx.doi.org/10.3390/nu14132721 |
_version_ | 1784743999840452608 |
---|---|
author | Charlot, Anouk Morel, Lucas Bringolf, Anthony Georg, Isabelle Charles, Anne-Laure Goupilleau, Fabienne Geny, Bernard Zoll, Joffrey |
author_facet | Charlot, Anouk Morel, Lucas Bringolf, Anthony Georg, Isabelle Charles, Anne-Laure Goupilleau, Fabienne Geny, Bernard Zoll, Joffrey |
author_sort | Charlot, Anouk |
collection | PubMed |
description | Background: Medium Chain Fatty Acids (MCFAs) are a dietary supplement that exhibit interesting properties, due to their smaller molecular size. The acute consumption of MCFAs is expected to enhance exercise performance. However, the short-term effects of MCFAs on endurance performance remains poorly understood. The aim of our study is to evaluate the octanoic acid (C8)-rich diet effect on endurance capacity, and to explore their molecular and cellular effects. Methods: C57BL/6J mice were fed with a chow diet (Control group) or an octanoic acid-rich diet (C8 diet) for 6 weeks. Spontaneous activity, submaximal and maximal exercise tests were carried out to characterize the exercise capacities of the mice. Beta-oxidation and mitochondrial biogenesis pathways were explored in skeletal muscle by RT-qPCR, Western Blot (Quadriceps) and histochemical staining (Gastrocnemius). Results: Mice fed with a C8-rich diet presented a higher spontaneous activity (p < 0.05) and endurance capacities (p < 0.05) than the control, but no effect on maximal effort was observed. They also presented changes in the skeletal muscle metabolic phenotype, with a higher number of the oxidative fibers, rich in mitochondria. At the molecular level, the C8-diet induced an AMPK activation (p < 0.05), associated with a significant increase in PGC1a and CS gene expression and protein levels. Conclusion: Our study provided evidence that C8-enrichment as a food supplementation improves endurance capacities and activates mitochondrial biogenesis pathways leading to higher skeletal muscle oxidative capacities. |
format | Online Article Text |
id | pubmed-9268503 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92685032022-07-09 Octanoic Acid-Enrichment Diet Improves Endurance Capacity and Reprograms Mitochondrial Biogenesis in Skeletal Muscle of Mice Charlot, Anouk Morel, Lucas Bringolf, Anthony Georg, Isabelle Charles, Anne-Laure Goupilleau, Fabienne Geny, Bernard Zoll, Joffrey Nutrients Article Background: Medium Chain Fatty Acids (MCFAs) are a dietary supplement that exhibit interesting properties, due to their smaller molecular size. The acute consumption of MCFAs is expected to enhance exercise performance. However, the short-term effects of MCFAs on endurance performance remains poorly understood. The aim of our study is to evaluate the octanoic acid (C8)-rich diet effect on endurance capacity, and to explore their molecular and cellular effects. Methods: C57BL/6J mice were fed with a chow diet (Control group) or an octanoic acid-rich diet (C8 diet) for 6 weeks. Spontaneous activity, submaximal and maximal exercise tests were carried out to characterize the exercise capacities of the mice. Beta-oxidation and mitochondrial biogenesis pathways were explored in skeletal muscle by RT-qPCR, Western Blot (Quadriceps) and histochemical staining (Gastrocnemius). Results: Mice fed with a C8-rich diet presented a higher spontaneous activity (p < 0.05) and endurance capacities (p < 0.05) than the control, but no effect on maximal effort was observed. They also presented changes in the skeletal muscle metabolic phenotype, with a higher number of the oxidative fibers, rich in mitochondria. At the molecular level, the C8-diet induced an AMPK activation (p < 0.05), associated with a significant increase in PGC1a and CS gene expression and protein levels. Conclusion: Our study provided evidence that C8-enrichment as a food supplementation improves endurance capacities and activates mitochondrial biogenesis pathways leading to higher skeletal muscle oxidative capacities. MDPI 2022-06-29 /pmc/articles/PMC9268503/ /pubmed/35807901 http://dx.doi.org/10.3390/nu14132721 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Charlot, Anouk Morel, Lucas Bringolf, Anthony Georg, Isabelle Charles, Anne-Laure Goupilleau, Fabienne Geny, Bernard Zoll, Joffrey Octanoic Acid-Enrichment Diet Improves Endurance Capacity and Reprograms Mitochondrial Biogenesis in Skeletal Muscle of Mice |
title | Octanoic Acid-Enrichment Diet Improves Endurance Capacity and Reprograms Mitochondrial Biogenesis in Skeletal Muscle of Mice |
title_full | Octanoic Acid-Enrichment Diet Improves Endurance Capacity and Reprograms Mitochondrial Biogenesis in Skeletal Muscle of Mice |
title_fullStr | Octanoic Acid-Enrichment Diet Improves Endurance Capacity and Reprograms Mitochondrial Biogenesis in Skeletal Muscle of Mice |
title_full_unstemmed | Octanoic Acid-Enrichment Diet Improves Endurance Capacity and Reprograms Mitochondrial Biogenesis in Skeletal Muscle of Mice |
title_short | Octanoic Acid-Enrichment Diet Improves Endurance Capacity and Reprograms Mitochondrial Biogenesis in Skeletal Muscle of Mice |
title_sort | octanoic acid-enrichment diet improves endurance capacity and reprograms mitochondrial biogenesis in skeletal muscle of mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268503/ https://www.ncbi.nlm.nih.gov/pubmed/35807901 http://dx.doi.org/10.3390/nu14132721 |
work_keys_str_mv | AT charlotanouk octanoicacidenrichmentdietimprovesendurancecapacityandreprogramsmitochondrialbiogenesisinskeletalmuscleofmice AT morellucas octanoicacidenrichmentdietimprovesendurancecapacityandreprogramsmitochondrialbiogenesisinskeletalmuscleofmice AT bringolfanthony octanoicacidenrichmentdietimprovesendurancecapacityandreprogramsmitochondrialbiogenesisinskeletalmuscleofmice AT georgisabelle octanoicacidenrichmentdietimprovesendurancecapacityandreprogramsmitochondrialbiogenesisinskeletalmuscleofmice AT charlesannelaure octanoicacidenrichmentdietimprovesendurancecapacityandreprogramsmitochondrialbiogenesisinskeletalmuscleofmice AT goupilleaufabienne octanoicacidenrichmentdietimprovesendurancecapacityandreprogramsmitochondrialbiogenesisinskeletalmuscleofmice AT genybernard octanoicacidenrichmentdietimprovesendurancecapacityandreprogramsmitochondrialbiogenesisinskeletalmuscleofmice AT zolljoffrey octanoicacidenrichmentdietimprovesendurancecapacityandreprogramsmitochondrialbiogenesisinskeletalmuscleofmice |