Cargando…
Anti-Inflammatory and Wound Healing Properties of Leaf and Rhizome Extracts from the Medicinal Plant Peucedanum ostruthium (L.) W. D. J. Koch
Peucedanum ostruthium (L.) W. D. J. Koch (Apiaceae) is a worldwide perennial herb native to the mountains of central Southern Europe. The rhizome has a long tradition in popular medicine, while ethnobotanical surveys have revealed local uses of leaves for superficial injuries. To experimentally vali...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268734/ https://www.ncbi.nlm.nih.gov/pubmed/35807516 http://dx.doi.org/10.3390/molecules27134271 |
_version_ | 1784744057874939904 |
---|---|
author | Danna, Cristina Bazzicalupo, Miriam Ingegneri, Mariarosaria Smeriglio, Antonella Trombetta, Domenico Burlando, Bruno Cornara, Laura |
author_facet | Danna, Cristina Bazzicalupo, Miriam Ingegneri, Mariarosaria Smeriglio, Antonella Trombetta, Domenico Burlando, Bruno Cornara, Laura |
author_sort | Danna, Cristina |
collection | PubMed |
description | Peucedanum ostruthium (L.) W. D. J. Koch (Apiaceae) is a worldwide perennial herb native to the mountains of central Southern Europe. The rhizome has a long tradition in popular medicine, while ethnobotanical surveys have revealed local uses of leaves for superficial injuries. To experimentally validate these uses, plant material was collected in the Gran Paradiso National Park, Aosta Valley, Italy, and the rhizome and leaves were micromorphologically and phytochemically characterized. Polyphenol-enriched hydroalcoholic rhizome and leaf extracts, used in cell-free assays, showed strong and concentration-dependent antioxidant and anti-inflammatory activities. In vitro tests revealed cyclooxygenase and lipoxygenase inhibition by the leaf extract, while the rhizome extract induced only lipoxygenase inhibition. MTT assays on HaCaT keratinocytes and L929 fibroblasts showed low cytotoxicity of extracts. In vitro scratch wound test on HaCaT resulted in a strong induction of wound closure with the leaf extract, while the effect of the rhizome extract was lower. The same test on L929 cells showed similar wound closure induction with both extracts. The results confirmed the traditional medicinal uses of the rhizome as an anti-inflammatory and wound healing remedy for superficial injuries but also highlighted that the leaves can be exploited for these purposes with equal or superior effectiveness. |
format | Online Article Text |
id | pubmed-9268734 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92687342022-07-09 Anti-Inflammatory and Wound Healing Properties of Leaf and Rhizome Extracts from the Medicinal Plant Peucedanum ostruthium (L.) W. D. J. Koch Danna, Cristina Bazzicalupo, Miriam Ingegneri, Mariarosaria Smeriglio, Antonella Trombetta, Domenico Burlando, Bruno Cornara, Laura Molecules Article Peucedanum ostruthium (L.) W. D. J. Koch (Apiaceae) is a worldwide perennial herb native to the mountains of central Southern Europe. The rhizome has a long tradition in popular medicine, while ethnobotanical surveys have revealed local uses of leaves for superficial injuries. To experimentally validate these uses, plant material was collected in the Gran Paradiso National Park, Aosta Valley, Italy, and the rhizome and leaves were micromorphologically and phytochemically characterized. Polyphenol-enriched hydroalcoholic rhizome and leaf extracts, used in cell-free assays, showed strong and concentration-dependent antioxidant and anti-inflammatory activities. In vitro tests revealed cyclooxygenase and lipoxygenase inhibition by the leaf extract, while the rhizome extract induced only lipoxygenase inhibition. MTT assays on HaCaT keratinocytes and L929 fibroblasts showed low cytotoxicity of extracts. In vitro scratch wound test on HaCaT resulted in a strong induction of wound closure with the leaf extract, while the effect of the rhizome extract was lower. The same test on L929 cells showed similar wound closure induction with both extracts. The results confirmed the traditional medicinal uses of the rhizome as an anti-inflammatory and wound healing remedy for superficial injuries but also highlighted that the leaves can be exploited for these purposes with equal or superior effectiveness. MDPI 2022-07-02 /pmc/articles/PMC9268734/ /pubmed/35807516 http://dx.doi.org/10.3390/molecules27134271 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Danna, Cristina Bazzicalupo, Miriam Ingegneri, Mariarosaria Smeriglio, Antonella Trombetta, Domenico Burlando, Bruno Cornara, Laura Anti-Inflammatory and Wound Healing Properties of Leaf and Rhizome Extracts from the Medicinal Plant Peucedanum ostruthium (L.) W. D. J. Koch |
title | Anti-Inflammatory and Wound Healing Properties of Leaf and Rhizome Extracts from the Medicinal Plant Peucedanum ostruthium (L.) W. D. J. Koch |
title_full | Anti-Inflammatory and Wound Healing Properties of Leaf and Rhizome Extracts from the Medicinal Plant Peucedanum ostruthium (L.) W. D. J. Koch |
title_fullStr | Anti-Inflammatory and Wound Healing Properties of Leaf and Rhizome Extracts from the Medicinal Plant Peucedanum ostruthium (L.) W. D. J. Koch |
title_full_unstemmed | Anti-Inflammatory and Wound Healing Properties of Leaf and Rhizome Extracts from the Medicinal Plant Peucedanum ostruthium (L.) W. D. J. Koch |
title_short | Anti-Inflammatory and Wound Healing Properties of Leaf and Rhizome Extracts from the Medicinal Plant Peucedanum ostruthium (L.) W. D. J. Koch |
title_sort | anti-inflammatory and wound healing properties of leaf and rhizome extracts from the medicinal plant peucedanum ostruthium (l.) w. d. j. koch |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268734/ https://www.ncbi.nlm.nih.gov/pubmed/35807516 http://dx.doi.org/10.3390/molecules27134271 |
work_keys_str_mv | AT dannacristina antiinflammatoryandwoundhealingpropertiesofleafandrhizomeextractsfromthemedicinalplantpeucedanumostruthiumlwdjkoch AT bazzicalupomiriam antiinflammatoryandwoundhealingpropertiesofleafandrhizomeextractsfromthemedicinalplantpeucedanumostruthiumlwdjkoch AT ingegnerimariarosaria antiinflammatoryandwoundhealingpropertiesofleafandrhizomeextractsfromthemedicinalplantpeucedanumostruthiumlwdjkoch AT smeriglioantonella antiinflammatoryandwoundhealingpropertiesofleafandrhizomeextractsfromthemedicinalplantpeucedanumostruthiumlwdjkoch AT trombettadomenico antiinflammatoryandwoundhealingpropertiesofleafandrhizomeextractsfromthemedicinalplantpeucedanumostruthiumlwdjkoch AT burlandobruno antiinflammatoryandwoundhealingpropertiesofleafandrhizomeextractsfromthemedicinalplantpeucedanumostruthiumlwdjkoch AT cornaralaura antiinflammatoryandwoundhealingpropertiesofleafandrhizomeextractsfromthemedicinalplantpeucedanumostruthiumlwdjkoch |