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Characterization of 3D Printed Metal-PLA Composite Scaffolds for Biomedical Applications
Three-dimensional printing is revolutionizing the development of scaffolds due to their rapid-prototyping characteristics. One of the most used techniques is fused filament fabrication (FFF), which is fast and compatible with a wide range of polymers, such as PolyLactic Acid (PLA). Mechanical proper...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268876/ https://www.ncbi.nlm.nih.gov/pubmed/35808799 http://dx.doi.org/10.3390/polym14132754 |
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author | Buj-Corral, Irene Sanz-Fraile, Héctor Ulldemolins, Anna Tejo-Otero, Aitor Domínguez-Fernández, Alejandro Almendros, Isaac Otero, Jorge |
author_facet | Buj-Corral, Irene Sanz-Fraile, Héctor Ulldemolins, Anna Tejo-Otero, Aitor Domínguez-Fernández, Alejandro Almendros, Isaac Otero, Jorge |
author_sort | Buj-Corral, Irene |
collection | PubMed |
description | Three-dimensional printing is revolutionizing the development of scaffolds due to their rapid-prototyping characteristics. One of the most used techniques is fused filament fabrication (FFF), which is fast and compatible with a wide range of polymers, such as PolyLactic Acid (PLA). Mechanical properties of the 3D printed polymeric scaffolds are often weak for certain applications. A potential solution is the development of composite materials. In the present work, metal-PLA composites have been tested as a material for 3D printing scaffolds. Three different materials were tested: copper-filled PLA, bronze-filled PLA, and steel-filled PLA. Disk-shaped samples were printed with linear infill patterns and line spacing of 0.6, 0.7, and 0.8 mm, respectively. The porosity of the samples was measured from cross-sectional images. Biocompatibility was assessed by culturing Human Bone Marrow-Derived Mesenchymal Stromal on the surface of the printed scaffolds. The results showed that, for identical line spacing value, the highest porosity corresponded to bronze-filled material and the lowest one to steel-filled material. Steel-filled PLA polymers showed good cytocompatibility without the need to coat the material with biomolecules. Moreover, human bone marrow-derived mesenchymal stromal cells differentiated towards osteoblasts when cultured on top of the developed scaffolds. Therefore, it can be concluded that steel-filled PLA bioprinted parts are valid scaffolds for bone tissue engineering. |
format | Online Article Text |
id | pubmed-9268876 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92688762022-07-09 Characterization of 3D Printed Metal-PLA Composite Scaffolds for Biomedical Applications Buj-Corral, Irene Sanz-Fraile, Héctor Ulldemolins, Anna Tejo-Otero, Aitor Domínguez-Fernández, Alejandro Almendros, Isaac Otero, Jorge Polymers (Basel) Communication Three-dimensional printing is revolutionizing the development of scaffolds due to their rapid-prototyping characteristics. One of the most used techniques is fused filament fabrication (FFF), which is fast and compatible with a wide range of polymers, such as PolyLactic Acid (PLA). Mechanical properties of the 3D printed polymeric scaffolds are often weak for certain applications. A potential solution is the development of composite materials. In the present work, metal-PLA composites have been tested as a material for 3D printing scaffolds. Three different materials were tested: copper-filled PLA, bronze-filled PLA, and steel-filled PLA. Disk-shaped samples were printed with linear infill patterns and line spacing of 0.6, 0.7, and 0.8 mm, respectively. The porosity of the samples was measured from cross-sectional images. Biocompatibility was assessed by culturing Human Bone Marrow-Derived Mesenchymal Stromal on the surface of the printed scaffolds. The results showed that, for identical line spacing value, the highest porosity corresponded to bronze-filled material and the lowest one to steel-filled material. Steel-filled PLA polymers showed good cytocompatibility without the need to coat the material with biomolecules. Moreover, human bone marrow-derived mesenchymal stromal cells differentiated towards osteoblasts when cultured on top of the developed scaffolds. Therefore, it can be concluded that steel-filled PLA bioprinted parts are valid scaffolds for bone tissue engineering. MDPI 2022-07-05 /pmc/articles/PMC9268876/ /pubmed/35808799 http://dx.doi.org/10.3390/polym14132754 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Buj-Corral, Irene Sanz-Fraile, Héctor Ulldemolins, Anna Tejo-Otero, Aitor Domínguez-Fernández, Alejandro Almendros, Isaac Otero, Jorge Characterization of 3D Printed Metal-PLA Composite Scaffolds for Biomedical Applications |
title | Characterization of 3D Printed Metal-PLA Composite Scaffolds for Biomedical Applications |
title_full | Characterization of 3D Printed Metal-PLA Composite Scaffolds for Biomedical Applications |
title_fullStr | Characterization of 3D Printed Metal-PLA Composite Scaffolds for Biomedical Applications |
title_full_unstemmed | Characterization of 3D Printed Metal-PLA Composite Scaffolds for Biomedical Applications |
title_short | Characterization of 3D Printed Metal-PLA Composite Scaffolds for Biomedical Applications |
title_sort | characterization of 3d printed metal-pla composite scaffolds for biomedical applications |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268876/ https://www.ncbi.nlm.nih.gov/pubmed/35808799 http://dx.doi.org/10.3390/polym14132754 |
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