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Optimizing the Surface Structural and Morphological Properties of Silk Thin Films via Ultra-Short Laser Texturing for Creation of Muscle Cell Matrix Model

Temporary scaffolds that mimic the extracellular matrix’s structure and provide a stable substratum for the natural growth of cells are an innovative trend in the field of tissue engineering. The aim of this study is to obtain and design porous 2D fibroin-based cell matrices by femtosecond laser-ind...

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Autores principales: Angelova, Liliya, Daskalova, Albena, Filipov, Emil, Vila, Xavier Monforte, Tomasch, Janine, Avdeev, Georgi, Teuschl-Woller, Andreas H., Buchvarov, Ivan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269134/
https://www.ncbi.nlm.nih.gov/pubmed/35808630
http://dx.doi.org/10.3390/polym14132584
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author Angelova, Liliya
Daskalova, Albena
Filipov, Emil
Vila, Xavier Monforte
Tomasch, Janine
Avdeev, Georgi
Teuschl-Woller, Andreas H.
Buchvarov, Ivan
author_facet Angelova, Liliya
Daskalova, Albena
Filipov, Emil
Vila, Xavier Monforte
Tomasch, Janine
Avdeev, Georgi
Teuschl-Woller, Andreas H.
Buchvarov, Ivan
author_sort Angelova, Liliya
collection PubMed
description Temporary scaffolds that mimic the extracellular matrix’s structure and provide a stable substratum for the natural growth of cells are an innovative trend in the field of tissue engineering. The aim of this study is to obtain and design porous 2D fibroin-based cell matrices by femtosecond laser-induced microstructuring for future applications in muscle tissue engineering. Ultra-fast laser treatment is a non-contact method, which generates controlled porosity—the creation of micro/nanostructures on the surface of the biopolymer that can strongly affect cell behavior, while the control over its surface characteristics has the potential of directing the growth of future muscle tissue in the desired direction. The laser structured 2D thin film matrices from silk were characterized by means of SEM, EDX, AFM, FTIR, Micro-Raman, XRD, and 3D-roughness analyses. A WCA evaluation and initial experiments with murine C2C12 myoblasts cells were also performed. The results show that by varying the laser parameters, a different structuring degree can be achieved through the initial lifting and ejection of the material around the area of laser interaction to generate porous channels with varying widths and depths. The proper optimization of the applied laser parameters can significantly improve the bioactive properties of the investigated 2D model of a muscle cell matrix.
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spelling pubmed-92691342022-07-09 Optimizing the Surface Structural and Morphological Properties of Silk Thin Films via Ultra-Short Laser Texturing for Creation of Muscle Cell Matrix Model Angelova, Liliya Daskalova, Albena Filipov, Emil Vila, Xavier Monforte Tomasch, Janine Avdeev, Georgi Teuschl-Woller, Andreas H. Buchvarov, Ivan Polymers (Basel) Article Temporary scaffolds that mimic the extracellular matrix’s structure and provide a stable substratum for the natural growth of cells are an innovative trend in the field of tissue engineering. The aim of this study is to obtain and design porous 2D fibroin-based cell matrices by femtosecond laser-induced microstructuring for future applications in muscle tissue engineering. Ultra-fast laser treatment is a non-contact method, which generates controlled porosity—the creation of micro/nanostructures on the surface of the biopolymer that can strongly affect cell behavior, while the control over its surface characteristics has the potential of directing the growth of future muscle tissue in the desired direction. The laser structured 2D thin film matrices from silk were characterized by means of SEM, EDX, AFM, FTIR, Micro-Raman, XRD, and 3D-roughness analyses. A WCA evaluation and initial experiments with murine C2C12 myoblasts cells were also performed. The results show that by varying the laser parameters, a different structuring degree can be achieved through the initial lifting and ejection of the material around the area of laser interaction to generate porous channels with varying widths and depths. The proper optimization of the applied laser parameters can significantly improve the bioactive properties of the investigated 2D model of a muscle cell matrix. MDPI 2022-06-25 /pmc/articles/PMC9269134/ /pubmed/35808630 http://dx.doi.org/10.3390/polym14132584 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Angelova, Liliya
Daskalova, Albena
Filipov, Emil
Vila, Xavier Monforte
Tomasch, Janine
Avdeev, Georgi
Teuschl-Woller, Andreas H.
Buchvarov, Ivan
Optimizing the Surface Structural and Morphological Properties of Silk Thin Films via Ultra-Short Laser Texturing for Creation of Muscle Cell Matrix Model
title Optimizing the Surface Structural and Morphological Properties of Silk Thin Films via Ultra-Short Laser Texturing for Creation of Muscle Cell Matrix Model
title_full Optimizing the Surface Structural and Morphological Properties of Silk Thin Films via Ultra-Short Laser Texturing for Creation of Muscle Cell Matrix Model
title_fullStr Optimizing the Surface Structural and Morphological Properties of Silk Thin Films via Ultra-Short Laser Texturing for Creation of Muscle Cell Matrix Model
title_full_unstemmed Optimizing the Surface Structural and Morphological Properties of Silk Thin Films via Ultra-Short Laser Texturing for Creation of Muscle Cell Matrix Model
title_short Optimizing the Surface Structural and Morphological Properties of Silk Thin Films via Ultra-Short Laser Texturing for Creation of Muscle Cell Matrix Model
title_sort optimizing the surface structural and morphological properties of silk thin films via ultra-short laser texturing for creation of muscle cell matrix model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269134/
https://www.ncbi.nlm.nih.gov/pubmed/35808630
http://dx.doi.org/10.3390/polym14132584
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