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Community succession and straw degradation characteristics using a microbial decomposer at low temperature
This study explored changes in the microbial community structure during straw degradation by a microbial decomposer, M44. The microbial community succession at different degradation periods was analyzed using MiSeq high-throughput sequencing. The results showed that 14 days after inoculation, the fi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269364/ https://www.ncbi.nlm.nih.gov/pubmed/35802565 http://dx.doi.org/10.1371/journal.pone.0270162 |
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author | Zhang, Xin Borjigin, Qinggeer Gao, Julin Yu, Xiaofang Zhang, Bizhou Hu, Shuping Han, Shengcai Liu, Ruizhi Zhang, Sainan |
author_facet | Zhang, Xin Borjigin, Qinggeer Gao, Julin Yu, Xiaofang Zhang, Bizhou Hu, Shuping Han, Shengcai Liu, Ruizhi Zhang, Sainan |
author_sort | Zhang, Xin |
collection | PubMed |
description | This study explored changes in the microbial community structure during straw degradation by a microbial decomposer, M44. The microbial community succession at different degradation periods was analyzed using MiSeq high-throughput sequencing. The results showed that 14 days after inoculation, the filter paper enzyme and endoglucanase activities increased to 2.55 U·mL(-1) and 2.34 U·mL(-1). The xylanase, laccase, and lignin peroxidase activities rose to 9.86 U·mL(-1), 132.16 U·L(-1), and 85.43 U·L(-1) after 28 d, which was consistent with changes in the straw degradation rate. The degradation rates of straw, lignin, cellulose, and hemicellulose were 31.43%, 13.67%, 25.04%, and 21.69%, respectively, after 28 d of fermentation at 15°C. Proteobacteria, Firmicutes, and Bacteroidetes were the main bacterial species in samples at different degradation stages. The dominant genera included Pseudomonas, Delftia, and Paenibacillus during the initial stage (1 d, 7 d) and the mid-term stage (14 d). The key functional microbes during the late stage (21 d, 28 d) were Rhizobium, Chryseobacterium, Sphingobacterium, Brevundimonas, and Devosia. Changes in the bacterial consortium structure and straw degradation characteristics during different degradation periods were clarified to provide a theoretical basis for the rational utilization of microbial decomposer M44. |
format | Online Article Text |
id | pubmed-9269364 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-92693642022-07-09 Community succession and straw degradation characteristics using a microbial decomposer at low temperature Zhang, Xin Borjigin, Qinggeer Gao, Julin Yu, Xiaofang Zhang, Bizhou Hu, Shuping Han, Shengcai Liu, Ruizhi Zhang, Sainan PLoS One Research Article This study explored changes in the microbial community structure during straw degradation by a microbial decomposer, M44. The microbial community succession at different degradation periods was analyzed using MiSeq high-throughput sequencing. The results showed that 14 days after inoculation, the filter paper enzyme and endoglucanase activities increased to 2.55 U·mL(-1) and 2.34 U·mL(-1). The xylanase, laccase, and lignin peroxidase activities rose to 9.86 U·mL(-1), 132.16 U·L(-1), and 85.43 U·L(-1) after 28 d, which was consistent with changes in the straw degradation rate. The degradation rates of straw, lignin, cellulose, and hemicellulose were 31.43%, 13.67%, 25.04%, and 21.69%, respectively, after 28 d of fermentation at 15°C. Proteobacteria, Firmicutes, and Bacteroidetes were the main bacterial species in samples at different degradation stages. The dominant genera included Pseudomonas, Delftia, and Paenibacillus during the initial stage (1 d, 7 d) and the mid-term stage (14 d). The key functional microbes during the late stage (21 d, 28 d) were Rhizobium, Chryseobacterium, Sphingobacterium, Brevundimonas, and Devosia. Changes in the bacterial consortium structure and straw degradation characteristics during different degradation periods were clarified to provide a theoretical basis for the rational utilization of microbial decomposer M44. Public Library of Science 2022-07-08 /pmc/articles/PMC9269364/ /pubmed/35802565 http://dx.doi.org/10.1371/journal.pone.0270162 Text en © 2022 Zhang et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zhang, Xin Borjigin, Qinggeer Gao, Julin Yu, Xiaofang Zhang, Bizhou Hu, Shuping Han, Shengcai Liu, Ruizhi Zhang, Sainan Community succession and straw degradation characteristics using a microbial decomposer at low temperature |
title | Community succession and straw degradation characteristics using a microbial decomposer at low temperature |
title_full | Community succession and straw degradation characteristics using a microbial decomposer at low temperature |
title_fullStr | Community succession and straw degradation characteristics using a microbial decomposer at low temperature |
title_full_unstemmed | Community succession and straw degradation characteristics using a microbial decomposer at low temperature |
title_short | Community succession and straw degradation characteristics using a microbial decomposer at low temperature |
title_sort | community succession and straw degradation characteristics using a microbial decomposer at low temperature |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269364/ https://www.ncbi.nlm.nih.gov/pubmed/35802565 http://dx.doi.org/10.1371/journal.pone.0270162 |
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