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Effects of multigenerational imidacloprid and thiamethoxam stress on metabolism and physiology of Aphis glycines Matsumura (Hemiptera: Aphididae)
The soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), a primary pest of soybean, poses a severe threat to soybean production. In this study, the 4(th) instar nymphs were exposed to the LC(50) and LC(30) of imidacloprid and thiamethoxam from F0 to F4 generations to evaluate the activiti...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269462/ https://www.ncbi.nlm.nih.gov/pubmed/35802579 http://dx.doi.org/10.1371/journal.pone.0271069 |
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author | Zhang, Aonan Zhou, Wenjing Wu, Dongxue Han, Lanlan Zhao, Kuijun |
author_facet | Zhang, Aonan Zhou, Wenjing Wu, Dongxue Han, Lanlan Zhao, Kuijun |
author_sort | Zhang, Aonan |
collection | PubMed |
description | The soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), a primary pest of soybean, poses a severe threat to soybean production. In this study, the 4(th) instar nymphs were exposed to the LC(50) and LC(30) of imidacloprid and thiamethoxam from F0 to F4 generations to evaluate the activities of peroxidase, pyruvate kinase, and trehalase using microassay. We found that peroxidase and pyruvate kinase activities in soybean aphids increased rapidly, first to peak and then decreased slowly generation by generation under imidacloprid and thiamethoxam stress. In contrast, the trehalase activity was significantly decreased in F1 to F5 generations when treated with the LC(50) and LC(30) and imidacloprid and thiamethoxam compared to control. In addition, the Enzyme-Linked Immunosorbent Assay (ELISA) was used to monitor the changes in molting and juvenile hormone expressions of the soybean aphids in each generation (F1-F5). The expression of juvenile hormone in soybean aphids was increased significantly in each generation under continuous stress of imidacloprid and thiamethoxam LC(50) imidacloprid and LC(50) thiamethoxam inhibited the expression of molting hormones in soybean aphids of each generation. LC(30) imidacloprid or LC(30) thiamethoxam significantly stimulated the expression of molting hormone in the 1(st) and 2(nd) instar nymphs in each generation. In this paper, the differences in antioxidant regulation, energy metabolism intensity, and hormone expression of multi-generation soybean aphids were monitored under continuous stress of imidacloprid and thiamethoxam. Our results revealed the effects of continuous insecticide stress on the main endogenous substances. Further, they clarified the regulation rules of resistance in soybean aphids, providing a reference for efficient control with imidacloprid and thiamethoxam. |
format | Online Article Text |
id | pubmed-9269462 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-92694622022-07-09 Effects of multigenerational imidacloprid and thiamethoxam stress on metabolism and physiology of Aphis glycines Matsumura (Hemiptera: Aphididae) Zhang, Aonan Zhou, Wenjing Wu, Dongxue Han, Lanlan Zhao, Kuijun PLoS One Research Article The soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), a primary pest of soybean, poses a severe threat to soybean production. In this study, the 4(th) instar nymphs were exposed to the LC(50) and LC(30) of imidacloprid and thiamethoxam from F0 to F4 generations to evaluate the activities of peroxidase, pyruvate kinase, and trehalase using microassay. We found that peroxidase and pyruvate kinase activities in soybean aphids increased rapidly, first to peak and then decreased slowly generation by generation under imidacloprid and thiamethoxam stress. In contrast, the trehalase activity was significantly decreased in F1 to F5 generations when treated with the LC(50) and LC(30) and imidacloprid and thiamethoxam compared to control. In addition, the Enzyme-Linked Immunosorbent Assay (ELISA) was used to monitor the changes in molting and juvenile hormone expressions of the soybean aphids in each generation (F1-F5). The expression of juvenile hormone in soybean aphids was increased significantly in each generation under continuous stress of imidacloprid and thiamethoxam LC(50) imidacloprid and LC(50) thiamethoxam inhibited the expression of molting hormones in soybean aphids of each generation. LC(30) imidacloprid or LC(30) thiamethoxam significantly stimulated the expression of molting hormone in the 1(st) and 2(nd) instar nymphs in each generation. In this paper, the differences in antioxidant regulation, energy metabolism intensity, and hormone expression of multi-generation soybean aphids were monitored under continuous stress of imidacloprid and thiamethoxam. Our results revealed the effects of continuous insecticide stress on the main endogenous substances. Further, they clarified the regulation rules of resistance in soybean aphids, providing a reference for efficient control with imidacloprid and thiamethoxam. Public Library of Science 2022-07-08 /pmc/articles/PMC9269462/ /pubmed/35802579 http://dx.doi.org/10.1371/journal.pone.0271069 Text en © 2022 Zhang et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zhang, Aonan Zhou, Wenjing Wu, Dongxue Han, Lanlan Zhao, Kuijun Effects of multigenerational imidacloprid and thiamethoxam stress on metabolism and physiology of Aphis glycines Matsumura (Hemiptera: Aphididae) |
title | Effects of multigenerational imidacloprid and thiamethoxam stress on metabolism and physiology of Aphis glycines Matsumura (Hemiptera: Aphididae) |
title_full | Effects of multigenerational imidacloprid and thiamethoxam stress on metabolism and physiology of Aphis glycines Matsumura (Hemiptera: Aphididae) |
title_fullStr | Effects of multigenerational imidacloprid and thiamethoxam stress on metabolism and physiology of Aphis glycines Matsumura (Hemiptera: Aphididae) |
title_full_unstemmed | Effects of multigenerational imidacloprid and thiamethoxam stress on metabolism and physiology of Aphis glycines Matsumura (Hemiptera: Aphididae) |
title_short | Effects of multigenerational imidacloprid and thiamethoxam stress on metabolism and physiology of Aphis glycines Matsumura (Hemiptera: Aphididae) |
title_sort | effects of multigenerational imidacloprid and thiamethoxam stress on metabolism and physiology of aphis glycines matsumura (hemiptera: aphididae) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269462/ https://www.ncbi.nlm.nih.gov/pubmed/35802579 http://dx.doi.org/10.1371/journal.pone.0271069 |
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