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Cytocompatibility and Antibacterial Properties of Coaxial Electrospun Nanofibers Containing Ciprofloxacin and Indomethacin Drugs

A coaxial nanofibrous scaffold of poly (ε-caprolactone) and gelatin/cellulose acetate encapsulating anti-inflammatory and antibacterial drugs was co-electrospun for skin tissue regeneration. Indomethacin and ciprofloxacin as model drugs were added to the core and the shell solutions, respectively. T...

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Detalles Bibliográficos
Autores principales: Khalili, Shahla, Ghane, Nazanin, Nouri Khorasani, Saied, Heydari, Fariba, Atwal, Arjan, Davoodi, Pooya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269477/
https://www.ncbi.nlm.nih.gov/pubmed/35808610
http://dx.doi.org/10.3390/polym14132565
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author Khalili, Shahla
Ghane, Nazanin
Nouri Khorasani, Saied
Heydari, Fariba
Atwal, Arjan
Davoodi, Pooya
author_facet Khalili, Shahla
Ghane, Nazanin
Nouri Khorasani, Saied
Heydari, Fariba
Atwal, Arjan
Davoodi, Pooya
author_sort Khalili, Shahla
collection PubMed
description A coaxial nanofibrous scaffold of poly (ε-caprolactone) and gelatin/cellulose acetate encapsulating anti-inflammatory and antibacterial drugs was co-electrospun for skin tissue regeneration. Indomethacin and ciprofloxacin as model drugs were added to the core and the shell solutions, respectively. The effect of the drugs’ presence and crosslinking on the scaffold properties was investigated. TEM images confirmed the core–shell structure of the scaffold. The fiber diameter and the pore size of the scaffold increased after crosslinking. The tensile properties of the scaffold improved after crosslinking. The crosslinked scaffold illustrated a higher rate of swelling, and a lower rate of degradation and drug release compared to the uncrosslinked one. Fitting the release data into the Peppas equation showed that Fickian diffusion was the dominant mechanism of drug release from the scaffolds. The results of biocompatibility evaluations showed no cytotoxicity and suitable adhesion and cell growth on the prepared core–shell structure. The antibacterial activity of the scaffolds was studied against one of the most common pathogens in skin wounds, where the existence of ciprofloxacin could prevent the growth of the Staphylococcus aureus bacteria around the scaffold. The obtained results suggested a new coaxial nanofibrous scaffold as a promising candidate for simultaneous tissue regeneration and controlled drug release.
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spelling pubmed-92694772022-07-09 Cytocompatibility and Antibacterial Properties of Coaxial Electrospun Nanofibers Containing Ciprofloxacin and Indomethacin Drugs Khalili, Shahla Ghane, Nazanin Nouri Khorasani, Saied Heydari, Fariba Atwal, Arjan Davoodi, Pooya Polymers (Basel) Article A coaxial nanofibrous scaffold of poly (ε-caprolactone) and gelatin/cellulose acetate encapsulating anti-inflammatory and antibacterial drugs was co-electrospun for skin tissue regeneration. Indomethacin and ciprofloxacin as model drugs were added to the core and the shell solutions, respectively. The effect of the drugs’ presence and crosslinking on the scaffold properties was investigated. TEM images confirmed the core–shell structure of the scaffold. The fiber diameter and the pore size of the scaffold increased after crosslinking. The tensile properties of the scaffold improved after crosslinking. The crosslinked scaffold illustrated a higher rate of swelling, and a lower rate of degradation and drug release compared to the uncrosslinked one. Fitting the release data into the Peppas equation showed that Fickian diffusion was the dominant mechanism of drug release from the scaffolds. The results of biocompatibility evaluations showed no cytotoxicity and suitable adhesion and cell growth on the prepared core–shell structure. The antibacterial activity of the scaffolds was studied against one of the most common pathogens in skin wounds, where the existence of ciprofloxacin could prevent the growth of the Staphylococcus aureus bacteria around the scaffold. The obtained results suggested a new coaxial nanofibrous scaffold as a promising candidate for simultaneous tissue regeneration and controlled drug release. MDPI 2022-06-24 /pmc/articles/PMC9269477/ /pubmed/35808610 http://dx.doi.org/10.3390/polym14132565 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Khalili, Shahla
Ghane, Nazanin
Nouri Khorasani, Saied
Heydari, Fariba
Atwal, Arjan
Davoodi, Pooya
Cytocompatibility and Antibacterial Properties of Coaxial Electrospun Nanofibers Containing Ciprofloxacin and Indomethacin Drugs
title Cytocompatibility and Antibacterial Properties of Coaxial Electrospun Nanofibers Containing Ciprofloxacin and Indomethacin Drugs
title_full Cytocompatibility and Antibacterial Properties of Coaxial Electrospun Nanofibers Containing Ciprofloxacin and Indomethacin Drugs
title_fullStr Cytocompatibility and Antibacterial Properties of Coaxial Electrospun Nanofibers Containing Ciprofloxacin and Indomethacin Drugs
title_full_unstemmed Cytocompatibility and Antibacterial Properties of Coaxial Electrospun Nanofibers Containing Ciprofloxacin and Indomethacin Drugs
title_short Cytocompatibility and Antibacterial Properties of Coaxial Electrospun Nanofibers Containing Ciprofloxacin and Indomethacin Drugs
title_sort cytocompatibility and antibacterial properties of coaxial electrospun nanofibers containing ciprofloxacin and indomethacin drugs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269477/
https://www.ncbi.nlm.nih.gov/pubmed/35808610
http://dx.doi.org/10.3390/polym14132565
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