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Comparative LC–LTQ–MS–MS Analysis of the Leaf Extracts of Lantana camara and Lantana montevidensis Growing in Egypt with Insights into Their Antioxidant, Anti-Inflammatory, and Cytotoxic Activities
Lantana camara L. and Lantana montevidensis Briq. (F. Verbenaceae) are invasive ornamental weeds native to the tropical regions of Africa and America. The leaves of both species have been traditionally used as infusions for treating fever, rheumatism, and cancer. LC–MS–MS-guided profiling of the met...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269492/ https://www.ncbi.nlm.nih.gov/pubmed/35807651 http://dx.doi.org/10.3390/plants11131699 |
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author | El-Din, Mariam I. Gamal Fahmy, Nouran M. Wu, Fulin Salem, Maha M. Khattab, Omar M. El-Seedi, Hesham R. Korinek, Michal Hwang, Tsong-Long Osman, Ahmed K. El-Shazly, Mohamed Fayez, Shaimaa |
author_facet | El-Din, Mariam I. Gamal Fahmy, Nouran M. Wu, Fulin Salem, Maha M. Khattab, Omar M. El-Seedi, Hesham R. Korinek, Michal Hwang, Tsong-Long Osman, Ahmed K. El-Shazly, Mohamed Fayez, Shaimaa |
author_sort | El-Din, Mariam I. Gamal |
collection | PubMed |
description | Lantana camara L. and Lantana montevidensis Briq. (F. Verbenaceae) are invasive ornamental weeds native to the tropical regions of Africa and America. The leaves of both species have been traditionally used as infusions for treating fever, rheumatism, and cancer. LC–MS–MS-guided profiling of the methanolic extracts of the leaves of L. camara and L. montevidensis growing in Egypt led to the putative identification of 59 compounds belonging to terpenoids, flavonoids, iridoid glycosides, phenolic acids, and their derivatives. The in-vitro antioxidants and anti-inflammatory and anticancer activities of the two extracts were investigated. L. camara and L. montevidensis inhibited DPPH(•) (IC(50) = 34.01 ± 1.32 and 47.43 ± 1.74 µg/mL), ABTS(+) (IC(50) = 30.73 ± 1.42 and 40.37 ± 1.51 µg/mL), and superoxide anion (IC(50) = 1.57 ± 0.19 and 1.31 ± 0.14 μg/mL) free radicals. A potent anti-inflammatory effect was observed for both species through the inhibition of elastase release in fMLF/CB-induced human neutrophils (IC(50) = 2.40 ± 0.16 and 1.90 ± 0.07 μg/mL). The extracts showed significant cytotoxic activity against a panel of cancer cell lines with the most potent activity against Caco cells (IC(50) = 45.65 ± 1.64 and 40.67 ± 1.52 µg/mL for L. camara and L. montevidensis, respectively). Western blotting supported by FACS analysis revealed that the extracts inhibited cancer cell proliferation, reduced metastasis, and induced apoptosis resulting in cell cycle arrest. This was achieved via increasing mRNA and protein expressions of p53 and GSK-3β as well as decreasing the expression of PI3K, Akt, and cyclin D1. |
format | Online Article Text |
id | pubmed-9269492 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-92694922022-07-09 Comparative LC–LTQ–MS–MS Analysis of the Leaf Extracts of Lantana camara and Lantana montevidensis Growing in Egypt with Insights into Their Antioxidant, Anti-Inflammatory, and Cytotoxic Activities El-Din, Mariam I. Gamal Fahmy, Nouran M. Wu, Fulin Salem, Maha M. Khattab, Omar M. El-Seedi, Hesham R. Korinek, Michal Hwang, Tsong-Long Osman, Ahmed K. El-Shazly, Mohamed Fayez, Shaimaa Plants (Basel) Article Lantana camara L. and Lantana montevidensis Briq. (F. Verbenaceae) are invasive ornamental weeds native to the tropical regions of Africa and America. The leaves of both species have been traditionally used as infusions for treating fever, rheumatism, and cancer. LC–MS–MS-guided profiling of the methanolic extracts of the leaves of L. camara and L. montevidensis growing in Egypt led to the putative identification of 59 compounds belonging to terpenoids, flavonoids, iridoid glycosides, phenolic acids, and their derivatives. The in-vitro antioxidants and anti-inflammatory and anticancer activities of the two extracts were investigated. L. camara and L. montevidensis inhibited DPPH(•) (IC(50) = 34.01 ± 1.32 and 47.43 ± 1.74 µg/mL), ABTS(+) (IC(50) = 30.73 ± 1.42 and 40.37 ± 1.51 µg/mL), and superoxide anion (IC(50) = 1.57 ± 0.19 and 1.31 ± 0.14 μg/mL) free radicals. A potent anti-inflammatory effect was observed for both species through the inhibition of elastase release in fMLF/CB-induced human neutrophils (IC(50) = 2.40 ± 0.16 and 1.90 ± 0.07 μg/mL). The extracts showed significant cytotoxic activity against a panel of cancer cell lines with the most potent activity against Caco cells (IC(50) = 45.65 ± 1.64 and 40.67 ± 1.52 µg/mL for L. camara and L. montevidensis, respectively). Western blotting supported by FACS analysis revealed that the extracts inhibited cancer cell proliferation, reduced metastasis, and induced apoptosis resulting in cell cycle arrest. This was achieved via increasing mRNA and protein expressions of p53 and GSK-3β as well as decreasing the expression of PI3K, Akt, and cyclin D1. MDPI 2022-06-27 /pmc/articles/PMC9269492/ /pubmed/35807651 http://dx.doi.org/10.3390/plants11131699 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article El-Din, Mariam I. Gamal Fahmy, Nouran M. Wu, Fulin Salem, Maha M. Khattab, Omar M. El-Seedi, Hesham R. Korinek, Michal Hwang, Tsong-Long Osman, Ahmed K. El-Shazly, Mohamed Fayez, Shaimaa Comparative LC–LTQ–MS–MS Analysis of the Leaf Extracts of Lantana camara and Lantana montevidensis Growing in Egypt with Insights into Their Antioxidant, Anti-Inflammatory, and Cytotoxic Activities |
title | Comparative LC–LTQ–MS–MS Analysis of the Leaf Extracts of Lantana camara and Lantana montevidensis Growing in Egypt with Insights into Their Antioxidant, Anti-Inflammatory, and Cytotoxic Activities |
title_full | Comparative LC–LTQ–MS–MS Analysis of the Leaf Extracts of Lantana camara and Lantana montevidensis Growing in Egypt with Insights into Their Antioxidant, Anti-Inflammatory, and Cytotoxic Activities |
title_fullStr | Comparative LC–LTQ–MS–MS Analysis of the Leaf Extracts of Lantana camara and Lantana montevidensis Growing in Egypt with Insights into Their Antioxidant, Anti-Inflammatory, and Cytotoxic Activities |
title_full_unstemmed | Comparative LC–LTQ–MS–MS Analysis of the Leaf Extracts of Lantana camara and Lantana montevidensis Growing in Egypt with Insights into Their Antioxidant, Anti-Inflammatory, and Cytotoxic Activities |
title_short | Comparative LC–LTQ–MS–MS Analysis of the Leaf Extracts of Lantana camara and Lantana montevidensis Growing in Egypt with Insights into Their Antioxidant, Anti-Inflammatory, and Cytotoxic Activities |
title_sort | comparative lc–ltq–ms–ms analysis of the leaf extracts of lantana camara and lantana montevidensis growing in egypt with insights into their antioxidant, anti-inflammatory, and cytotoxic activities |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269492/ https://www.ncbi.nlm.nih.gov/pubmed/35807651 http://dx.doi.org/10.3390/plants11131699 |
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