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Real Time Water-In-Oil Emulsion Size Measurement in Optofluidic Channels

In this work, we investigated a platform for real-time emulsion droplet detection and size measurement in optofluidic platforms. An 8.2 µm core diameter input optical fiber and a multi-mode Gradient Refractive Index (GRIN) output fiber were integrated into an acrylic microfluidic channel platform co...

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Detalles Bibliográficos
Autores principales: Schianti, Juliana N., Abe, Igor Y., Alayo, Marco I., Carvalho, Daniel O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269678/
https://www.ncbi.nlm.nih.gov/pubmed/35808495
http://dx.doi.org/10.3390/s22134999
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author Schianti, Juliana N.
Abe, Igor Y.
Alayo, Marco I.
Carvalho, Daniel O.
author_facet Schianti, Juliana N.
Abe, Igor Y.
Alayo, Marco I.
Carvalho, Daniel O.
author_sort Schianti, Juliana N.
collection PubMed
description In this work, we investigated a platform for real-time emulsion droplet detection and size measurement in optofluidic platforms. An 8.2 µm core diameter input optical fiber and a multi-mode Gradient Refractive Index (GRIN) output fiber were integrated into an acrylic microfluidic channel platform consisting of three layers. Water-in-oil emulsions were investigated, since relevant applications have emerged in the recent past for these types of emulsions, such as drug encapsulation as well as droplet-based Polymerase Chain Reaction (PCR) amplification of DNA, among others. The main contribution of this work is in understanding the main physical phenomena (i.e., total internal reflection, refraction, and interference) behind the complex transmittance pattern obtained for these droplets. For this purpose, a frequency domain electromagnetic wave propagation modelling of the structure using the Finite Element Method (FEM) was used along with experimental measurements.
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spelling pubmed-92696782022-07-09 Real Time Water-In-Oil Emulsion Size Measurement in Optofluidic Channels Schianti, Juliana N. Abe, Igor Y. Alayo, Marco I. Carvalho, Daniel O. Sensors (Basel) Article In this work, we investigated a platform for real-time emulsion droplet detection and size measurement in optofluidic platforms. An 8.2 µm core diameter input optical fiber and a multi-mode Gradient Refractive Index (GRIN) output fiber were integrated into an acrylic microfluidic channel platform consisting of three layers. Water-in-oil emulsions were investigated, since relevant applications have emerged in the recent past for these types of emulsions, such as drug encapsulation as well as droplet-based Polymerase Chain Reaction (PCR) amplification of DNA, among others. The main contribution of this work is in understanding the main physical phenomena (i.e., total internal reflection, refraction, and interference) behind the complex transmittance pattern obtained for these droplets. For this purpose, a frequency domain electromagnetic wave propagation modelling of the structure using the Finite Element Method (FEM) was used along with experimental measurements. MDPI 2022-07-02 /pmc/articles/PMC9269678/ /pubmed/35808495 http://dx.doi.org/10.3390/s22134999 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Schianti, Juliana N.
Abe, Igor Y.
Alayo, Marco I.
Carvalho, Daniel O.
Real Time Water-In-Oil Emulsion Size Measurement in Optofluidic Channels
title Real Time Water-In-Oil Emulsion Size Measurement in Optofluidic Channels
title_full Real Time Water-In-Oil Emulsion Size Measurement in Optofluidic Channels
title_fullStr Real Time Water-In-Oil Emulsion Size Measurement in Optofluidic Channels
title_full_unstemmed Real Time Water-In-Oil Emulsion Size Measurement in Optofluidic Channels
title_short Real Time Water-In-Oil Emulsion Size Measurement in Optofluidic Channels
title_sort real time water-in-oil emulsion size measurement in optofluidic channels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269678/
https://www.ncbi.nlm.nih.gov/pubmed/35808495
http://dx.doi.org/10.3390/s22134999
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