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Dissemination of carbapenemase-producing Enterobacterales in the community of Rawalpindi, Pakistan
Carbapenems are considered last-line beta-lactams for the treatment of infections caused by multidrug-resistant Gram-negative bacteria. However, their activity is compromised by the rising prevalence of carbapenemase-producing Enterobacterales (CPE), which are especially marked in the Indian subcont...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269877/ https://www.ncbi.nlm.nih.gov/pubmed/35802735 http://dx.doi.org/10.1371/journal.pone.0270707 |
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author | Habib, Amina Lo, Stéphane Villageois-Tran, Khanh Petitjean, Marie Malik, Shaheen Akhtar Armand-Lefèvre, Laurence Ruppé, Etienne Zahra, Rabaab |
author_facet | Habib, Amina Lo, Stéphane Villageois-Tran, Khanh Petitjean, Marie Malik, Shaheen Akhtar Armand-Lefèvre, Laurence Ruppé, Etienne Zahra, Rabaab |
author_sort | Habib, Amina |
collection | PubMed |
description | Carbapenems are considered last-line beta-lactams for the treatment of infections caused by multidrug-resistant Gram-negative bacteria. However, their activity is compromised by the rising prevalence of carbapenemase-producing Enterobacterales (CPE), which are especially marked in the Indian subcontinent. In Pakistan, previous reports have warned about the possible spread of CPE in the community, but data are still partial. This study was carried out to analyse the prevalence of CPE, the genetic characterisation, and phylogenetic links among the spreading CPE in the community. In this cohort study, we collected 306 rectal swabs from patients visiting Benazir Bhutto hospital, Rawalpindi. CPEs were screened by using ertapenem-supplemented MacConkey agar. Identification was performed by using conventional biochemical tests, and genomes were sequenced using Illumina chemistry. Antibiotic resistance genes, plasmid incompatibility groups, and Escherichia coli phylogroups were determined in silico. Sequence types were determined by using MLST tool. The prevalence of CPE carriage observed was 14.4% (44/306 samples). The most common carbapenemase-encoding gene was bla-(NDM-5) (n = 58) followed by bla(NDM-1) (n = 7), bla(NDM) (non-assigned variant, n = 4), bla(OXA-181) (n = 3), bla(OXA-232) (n = 3) and bla(NDM-7) (n = 1). Most of the CPE were E. coli (55/64, 86%), and the genomic analysis revealed a pauciclonal diffusion of E. coli with ST167 (n = 14), 405 (n = 10), 940 (n = 8), 648 (n = 6) and 617 (n = 5). We obtained a second sample from 94 patients during their hospital stay in whom carriage was negative at admission and found that 7 (7.4%) acquired a CPE. Our results indicate that the prevalence of CPE carriage in the Pakistani urban community was high and driven by the dissemination of some E. coli clones, with ST167 being the most frequent. The high CPE carriage in the community poses a serious public health threat and calls for implementation of adequate preventive measures. |
format | Online Article Text |
id | pubmed-9269877 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-92698772022-07-09 Dissemination of carbapenemase-producing Enterobacterales in the community of Rawalpindi, Pakistan Habib, Amina Lo, Stéphane Villageois-Tran, Khanh Petitjean, Marie Malik, Shaheen Akhtar Armand-Lefèvre, Laurence Ruppé, Etienne Zahra, Rabaab PLoS One Research Article Carbapenems are considered last-line beta-lactams for the treatment of infections caused by multidrug-resistant Gram-negative bacteria. However, their activity is compromised by the rising prevalence of carbapenemase-producing Enterobacterales (CPE), which are especially marked in the Indian subcontinent. In Pakistan, previous reports have warned about the possible spread of CPE in the community, but data are still partial. This study was carried out to analyse the prevalence of CPE, the genetic characterisation, and phylogenetic links among the spreading CPE in the community. In this cohort study, we collected 306 rectal swabs from patients visiting Benazir Bhutto hospital, Rawalpindi. CPEs were screened by using ertapenem-supplemented MacConkey agar. Identification was performed by using conventional biochemical tests, and genomes were sequenced using Illumina chemistry. Antibiotic resistance genes, plasmid incompatibility groups, and Escherichia coli phylogroups were determined in silico. Sequence types were determined by using MLST tool. The prevalence of CPE carriage observed was 14.4% (44/306 samples). The most common carbapenemase-encoding gene was bla-(NDM-5) (n = 58) followed by bla(NDM-1) (n = 7), bla(NDM) (non-assigned variant, n = 4), bla(OXA-181) (n = 3), bla(OXA-232) (n = 3) and bla(NDM-7) (n = 1). Most of the CPE were E. coli (55/64, 86%), and the genomic analysis revealed a pauciclonal diffusion of E. coli with ST167 (n = 14), 405 (n = 10), 940 (n = 8), 648 (n = 6) and 617 (n = 5). We obtained a second sample from 94 patients during their hospital stay in whom carriage was negative at admission and found that 7 (7.4%) acquired a CPE. Our results indicate that the prevalence of CPE carriage in the Pakistani urban community was high and driven by the dissemination of some E. coli clones, with ST167 being the most frequent. The high CPE carriage in the community poses a serious public health threat and calls for implementation of adequate preventive measures. Public Library of Science 2022-07-08 /pmc/articles/PMC9269877/ /pubmed/35802735 http://dx.doi.org/10.1371/journal.pone.0270707 Text en © 2022 Habib et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Habib, Amina Lo, Stéphane Villageois-Tran, Khanh Petitjean, Marie Malik, Shaheen Akhtar Armand-Lefèvre, Laurence Ruppé, Etienne Zahra, Rabaab Dissemination of carbapenemase-producing Enterobacterales in the community of Rawalpindi, Pakistan |
title | Dissemination of carbapenemase-producing Enterobacterales in the community of Rawalpindi, Pakistan |
title_full | Dissemination of carbapenemase-producing Enterobacterales in the community of Rawalpindi, Pakistan |
title_fullStr | Dissemination of carbapenemase-producing Enterobacterales in the community of Rawalpindi, Pakistan |
title_full_unstemmed | Dissemination of carbapenemase-producing Enterobacterales in the community of Rawalpindi, Pakistan |
title_short | Dissemination of carbapenemase-producing Enterobacterales in the community of Rawalpindi, Pakistan |
title_sort | dissemination of carbapenemase-producing enterobacterales in the community of rawalpindi, pakistan |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269877/ https://www.ncbi.nlm.nih.gov/pubmed/35802735 http://dx.doi.org/10.1371/journal.pone.0270707 |
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