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A label-free and low-power microelectronic impedance spectroscopy for characterization of exosomes

Electrical Impedance Spectroscopy (EIS) is a non-invasive and label-free technology that can characterize and discriminate cells based on their dielectric properties at a wide range of frequency. This characterization method has not been utilized for small extracellular vesicles (exosomes) with hete...

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Detalles Bibliográficos
Autores principales: Shi, Leilei, Esfandiari, Leyla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269907/
https://www.ncbi.nlm.nih.gov/pubmed/35802670
http://dx.doi.org/10.1371/journal.pone.0270844
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author Shi, Leilei
Esfandiari, Leyla
author_facet Shi, Leilei
Esfandiari, Leyla
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description Electrical Impedance Spectroscopy (EIS) is a non-invasive and label-free technology that can characterize and discriminate cells based on their dielectric properties at a wide range of frequency. This characterization method has not been utilized for small extracellular vesicles (exosomes) with heterogenous and nano-scale size distribution. Here, we developed a novel label-free microelectronic impedance spectroscopy for non-invasive and rapid characterization of exosomes based on their unique dielectric properties. The device is comprised of an insulator-based dielectrophoretic (iDEP) module for exosomes isolation followed by an impedance spectroscopy utilizing the embedded micro-electrodes. This device is capable of distinguishing between exosomes harvested from different cellular origins as the result of their unique membrane and cytosolic compositions at a wide range of frequency. Therefore, it has the potential to be further evolved as a rapid tool for characterization of pathogenic exosomes in clinical settings.
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spelling pubmed-92699072022-07-09 A label-free and low-power microelectronic impedance spectroscopy for characterization of exosomes Shi, Leilei Esfandiari, Leyla PLoS One Research Article Electrical Impedance Spectroscopy (EIS) is a non-invasive and label-free technology that can characterize and discriminate cells based on their dielectric properties at a wide range of frequency. This characterization method has not been utilized for small extracellular vesicles (exosomes) with heterogenous and nano-scale size distribution. Here, we developed a novel label-free microelectronic impedance spectroscopy for non-invasive and rapid characterization of exosomes based on their unique dielectric properties. The device is comprised of an insulator-based dielectrophoretic (iDEP) module for exosomes isolation followed by an impedance spectroscopy utilizing the embedded micro-electrodes. This device is capable of distinguishing between exosomes harvested from different cellular origins as the result of their unique membrane and cytosolic compositions at a wide range of frequency. Therefore, it has the potential to be further evolved as a rapid tool for characterization of pathogenic exosomes in clinical settings. Public Library of Science 2022-07-08 /pmc/articles/PMC9269907/ /pubmed/35802670 http://dx.doi.org/10.1371/journal.pone.0270844 Text en © 2022 Shi, Esfandiari https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Shi, Leilei
Esfandiari, Leyla
A label-free and low-power microelectronic impedance spectroscopy for characterization of exosomes
title A label-free and low-power microelectronic impedance spectroscopy for characterization of exosomes
title_full A label-free and low-power microelectronic impedance spectroscopy for characterization of exosomes
title_fullStr A label-free and low-power microelectronic impedance spectroscopy for characterization of exosomes
title_full_unstemmed A label-free and low-power microelectronic impedance spectroscopy for characterization of exosomes
title_short A label-free and low-power microelectronic impedance spectroscopy for characterization of exosomes
title_sort label-free and low-power microelectronic impedance spectroscopy for characterization of exosomes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269907/
https://www.ncbi.nlm.nih.gov/pubmed/35802670
http://dx.doi.org/10.1371/journal.pone.0270844
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