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A label-free and low-power microelectronic impedance spectroscopy for characterization of exosomes
Electrical Impedance Spectroscopy (EIS) is a non-invasive and label-free technology that can characterize and discriminate cells based on their dielectric properties at a wide range of frequency. This characterization method has not been utilized for small extracellular vesicles (exosomes) with hete...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269907/ https://www.ncbi.nlm.nih.gov/pubmed/35802670 http://dx.doi.org/10.1371/journal.pone.0270844 |
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author | Shi, Leilei Esfandiari, Leyla |
author_facet | Shi, Leilei Esfandiari, Leyla |
author_sort | Shi, Leilei |
collection | PubMed |
description | Electrical Impedance Spectroscopy (EIS) is a non-invasive and label-free technology that can characterize and discriminate cells based on their dielectric properties at a wide range of frequency. This characterization method has not been utilized for small extracellular vesicles (exosomes) with heterogenous and nano-scale size distribution. Here, we developed a novel label-free microelectronic impedance spectroscopy for non-invasive and rapid characterization of exosomes based on their unique dielectric properties. The device is comprised of an insulator-based dielectrophoretic (iDEP) module for exosomes isolation followed by an impedance spectroscopy utilizing the embedded micro-electrodes. This device is capable of distinguishing between exosomes harvested from different cellular origins as the result of their unique membrane and cytosolic compositions at a wide range of frequency. Therefore, it has the potential to be further evolved as a rapid tool for characterization of pathogenic exosomes in clinical settings. |
format | Online Article Text |
id | pubmed-9269907 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-92699072022-07-09 A label-free and low-power microelectronic impedance spectroscopy for characterization of exosomes Shi, Leilei Esfandiari, Leyla PLoS One Research Article Electrical Impedance Spectroscopy (EIS) is a non-invasive and label-free technology that can characterize and discriminate cells based on their dielectric properties at a wide range of frequency. This characterization method has not been utilized for small extracellular vesicles (exosomes) with heterogenous and nano-scale size distribution. Here, we developed a novel label-free microelectronic impedance spectroscopy for non-invasive and rapid characterization of exosomes based on their unique dielectric properties. The device is comprised of an insulator-based dielectrophoretic (iDEP) module for exosomes isolation followed by an impedance spectroscopy utilizing the embedded micro-electrodes. This device is capable of distinguishing between exosomes harvested from different cellular origins as the result of their unique membrane and cytosolic compositions at a wide range of frequency. Therefore, it has the potential to be further evolved as a rapid tool for characterization of pathogenic exosomes in clinical settings. Public Library of Science 2022-07-08 /pmc/articles/PMC9269907/ /pubmed/35802670 http://dx.doi.org/10.1371/journal.pone.0270844 Text en © 2022 Shi, Esfandiari https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Shi, Leilei Esfandiari, Leyla A label-free and low-power microelectronic impedance spectroscopy for characterization of exosomes |
title | A label-free and low-power microelectronic impedance spectroscopy for characterization of exosomes |
title_full | A label-free and low-power microelectronic impedance spectroscopy for characterization of exosomes |
title_fullStr | A label-free and low-power microelectronic impedance spectroscopy for characterization of exosomes |
title_full_unstemmed | A label-free and low-power microelectronic impedance spectroscopy for characterization of exosomes |
title_short | A label-free and low-power microelectronic impedance spectroscopy for characterization of exosomes |
title_sort | label-free and low-power microelectronic impedance spectroscopy for characterization of exosomes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269907/ https://www.ncbi.nlm.nih.gov/pubmed/35802670 http://dx.doi.org/10.1371/journal.pone.0270844 |
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