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Inhibitory Effect of Seawater Pearl Hydrolysate on UVA-Induced Photoaging of Human Skin Fibroblasts
This study is an investigation into the inhibitory effect of seawater pearl hydrolysate (SPH) on the UVA-induced photoaging of human skin fibroblast (HSF) cells, and the mechanism thereof. HSF cells were cultured and irradiated with a UVA 0–50 J·cm(−2) dose gradient. The cell inhibition rate was det...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270121/ https://www.ncbi.nlm.nih.gov/pubmed/35815281 http://dx.doi.org/10.1155/2022/1558288 |
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author | Han, Siyin Li, Hongxuan Luo, Fei Chen, Xin Cen, Yanhui Liu, Peng Chen, Zhenxing Lan, Taijin Lin, Jiang |
author_facet | Han, Siyin Li, Hongxuan Luo, Fei Chen, Xin Cen, Yanhui Liu, Peng Chen, Zhenxing Lan, Taijin Lin, Jiang |
author_sort | Han, Siyin |
collection | PubMed |
description | This study is an investigation into the inhibitory effect of seawater pearl hydrolysate (SPH) on the UVA-induced photoaging of human skin fibroblast (HSF) cells, and the mechanism thereof. HSF cells were cultured and irradiated with a UVA 0–50 J·cm(−2) dose gradient. The cell inhibition rate was detected using the CCK8 method, and the half-inhibitory dose was determined. Based on this, the dose of UVA irradiation for the follow-up experiment was selected to establish a photoaging model of the HSF cells. The cells were divided into a normal (N) group, UVA-irradiated (UVA) group, SPH low dose (SPHL) group, SPH medium dose (SPHM) group, and SPH high dose (SPHH) group. The photoaging model of HSF cells was established by UVA irradiation in the UVA, SPHL, SPHM, and SPHH groups; the SPHL, SPHM, and SPHH groups were treated with SPH at concentrations of 50, 100, and 200 mg·L(−1), respectively, at the same time. After 24 and 48 h of culture, the reactive oxygen species (ROS) level of the HSF cells was detected by flow cytometry, and the required culture time of the HSF cells for the follow-up experiment was selected. The malondialdehyde and glutathione contents, as well as the activities of the superoxide dismutase, catalase, and glutathione peroxidase in the HSF cells, were detected by biochemical methods. The levels of expression of MMP-1 and collagen I protein in HSF cells were detected by the western blot test, the extent of aging of HSF cells was detected by β-galactosidase staining, and the apoptosis level of HSF cells was detected by flow cytometry. The results show that SPH inhibits the UVA-induced photoaging of HSF cells in a dose-dependent manner within a certain concentration range, and the effect of a concentration of 200 mg·L(–1) was the most significant. The mechanism is related to improving the antioxidant activity of photoaging HSF cells to eliminate excessive ROS. It can inhibit apoptosis, reduce the protein expression of MMP-1, and effectively control the degradation of collagen I protein in photoaging HSF cells. Therefore, SPH offers potential for use in sunscreen cosmetics. |
format | Online Article Text |
id | pubmed-9270121 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-92701212022-07-09 Inhibitory Effect of Seawater Pearl Hydrolysate on UVA-Induced Photoaging of Human Skin Fibroblasts Han, Siyin Li, Hongxuan Luo, Fei Chen, Xin Cen, Yanhui Liu, Peng Chen, Zhenxing Lan, Taijin Lin, Jiang Evid Based Complement Alternat Med Research Article This study is an investigation into the inhibitory effect of seawater pearl hydrolysate (SPH) on the UVA-induced photoaging of human skin fibroblast (HSF) cells, and the mechanism thereof. HSF cells were cultured and irradiated with a UVA 0–50 J·cm(−2) dose gradient. The cell inhibition rate was detected using the CCK8 method, and the half-inhibitory dose was determined. Based on this, the dose of UVA irradiation for the follow-up experiment was selected to establish a photoaging model of the HSF cells. The cells were divided into a normal (N) group, UVA-irradiated (UVA) group, SPH low dose (SPHL) group, SPH medium dose (SPHM) group, and SPH high dose (SPHH) group. The photoaging model of HSF cells was established by UVA irradiation in the UVA, SPHL, SPHM, and SPHH groups; the SPHL, SPHM, and SPHH groups were treated with SPH at concentrations of 50, 100, and 200 mg·L(−1), respectively, at the same time. After 24 and 48 h of culture, the reactive oxygen species (ROS) level of the HSF cells was detected by flow cytometry, and the required culture time of the HSF cells for the follow-up experiment was selected. The malondialdehyde and glutathione contents, as well as the activities of the superoxide dismutase, catalase, and glutathione peroxidase in the HSF cells, were detected by biochemical methods. The levels of expression of MMP-1 and collagen I protein in HSF cells were detected by the western blot test, the extent of aging of HSF cells was detected by β-galactosidase staining, and the apoptosis level of HSF cells was detected by flow cytometry. The results show that SPH inhibits the UVA-induced photoaging of HSF cells in a dose-dependent manner within a certain concentration range, and the effect of a concentration of 200 mg·L(–1) was the most significant. The mechanism is related to improving the antioxidant activity of photoaging HSF cells to eliminate excessive ROS. It can inhibit apoptosis, reduce the protein expression of MMP-1, and effectively control the degradation of collagen I protein in photoaging HSF cells. Therefore, SPH offers potential for use in sunscreen cosmetics. Hindawi 2022-07-01 /pmc/articles/PMC9270121/ /pubmed/35815281 http://dx.doi.org/10.1155/2022/1558288 Text en Copyright © 2022 Siyin Han et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Han, Siyin Li, Hongxuan Luo, Fei Chen, Xin Cen, Yanhui Liu, Peng Chen, Zhenxing Lan, Taijin Lin, Jiang Inhibitory Effect of Seawater Pearl Hydrolysate on UVA-Induced Photoaging of Human Skin Fibroblasts |
title | Inhibitory Effect of Seawater Pearl Hydrolysate on UVA-Induced Photoaging of Human Skin Fibroblasts |
title_full | Inhibitory Effect of Seawater Pearl Hydrolysate on UVA-Induced Photoaging of Human Skin Fibroblasts |
title_fullStr | Inhibitory Effect of Seawater Pearl Hydrolysate on UVA-Induced Photoaging of Human Skin Fibroblasts |
title_full_unstemmed | Inhibitory Effect of Seawater Pearl Hydrolysate on UVA-Induced Photoaging of Human Skin Fibroblasts |
title_short | Inhibitory Effect of Seawater Pearl Hydrolysate on UVA-Induced Photoaging of Human Skin Fibroblasts |
title_sort | inhibitory effect of seawater pearl hydrolysate on uva-induced photoaging of human skin fibroblasts |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270121/ https://www.ncbi.nlm.nih.gov/pubmed/35815281 http://dx.doi.org/10.1155/2022/1558288 |
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