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A method for increasing electroporation competence of Gram-negative clinical isolates by polymyxin B nonapeptide

The study of clinically relevant bacterial pathogens relies on molecular and genetic approaches. However, the generally low transformation frequency among natural isolates poses technical hurdles to widely applying common methods in molecular biology, including transformation of large constructs, ch...

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Detalles Bibliográficos
Autores principales: Qin, Jilong, Hong, Yaoqin, Pullela, Karthik, Morona, Renato, Henderson, Ian R., Totsika, Makrina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270391/
https://www.ncbi.nlm.nih.gov/pubmed/35804085
http://dx.doi.org/10.1038/s41598-022-15997-8