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A method for increasing electroporation competence of Gram-negative clinical isolates by polymyxin B nonapeptide
The study of clinically relevant bacterial pathogens relies on molecular and genetic approaches. However, the generally low transformation frequency among natural isolates poses technical hurdles to widely applying common methods in molecular biology, including transformation of large constructs, ch...
Autores principales: | Qin, Jilong, Hong, Yaoqin, Pullela, Karthik, Morona, Renato, Henderson, Ian R., Totsika, Makrina |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270391/ https://www.ncbi.nlm.nih.gov/pubmed/35804085 http://dx.doi.org/10.1038/s41598-022-15997-8 |
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