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Expression and co‐localization of RFRP‐3 and kisspeptin during breeding and non‐breeding season in the hypothalamus of male rhesus monkey (Macaca mulatta)

PROPOSE: The mechanism that underpins how RFRP‐3 and kisspeptin interacts are not fully understood in higher primates. This study therefore set out to assess RFRP‐3 and kisspeptin expression and their morphological interactions in the breeding, and in the non‐breeding period in monkey hypothalamus....

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Autores principales: Khan, Safdar, Batool, Bakhtwar, Zubair, Hira, Bano, Riffat, Ahmad, Shakil, Shahab, Muhammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270642/
https://www.ncbi.nlm.nih.gov/pubmed/35847413
http://dx.doi.org/10.1002/rmb2.12479
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author Khan, Safdar
Batool, Bakhtwar
Zubair, Hira
Bano, Riffat
Ahmad, Shakil
Shahab, Muhammad
author_facet Khan, Safdar
Batool, Bakhtwar
Zubair, Hira
Bano, Riffat
Ahmad, Shakil
Shahab, Muhammad
author_sort Khan, Safdar
collection PubMed
description PROPOSE: The mechanism that underpins how RFRP‐3 and kisspeptin interacts are not fully understood in higher primates. This study therefore set out to assess RFRP‐3 and kisspeptin expression and their morphological interactions in the breeding, and in the non‐breeding period in monkey hypothalamus. METHODS: Eight mature male macaques (Macaca mulatta) in the breeding season (February; n = 4) and non‐breeding season (June; n = 4) were used. To reveal the expression and co‐localization of RFRP‐3 and kisspeptin, double‐labeled immunohistochemistry was performed. Testicular volume, sperm count, and plasma testosterone level were also measured to validate the breeding and non‐breeding paradigms. RESULTS: Testicular volume, plasma testosterone level, and sperm count showed a significant reduction during non‐breeding season. The number of kisspeptin‐positive cells was significantly increased during the breeding season (p < 0.05), whereas more RFRP‐3‐positive cell bodies were seen in the non‐breeding season (p < 0.01). Close contacts of RFRP‐3 fibers with kisspeptin cells showed no significant difference (p > 0.05) across seasons. However, co‐localization of RFRP‐3‐ir cell bodies onto kisspeptin IR cell bodies showed a statistical increase (p < 0.01) in non‐breeding season. CONCLUSION: In higher primates, RFRP‐3 decreases kisspeptin drives from the same cells to GnRH neurons in an autocrine manner causing suppression of the reproductive axis during the non‐breeding period.
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spelling pubmed-92706422022-07-14 Expression and co‐localization of RFRP‐3 and kisspeptin during breeding and non‐breeding season in the hypothalamus of male rhesus monkey (Macaca mulatta) Khan, Safdar Batool, Bakhtwar Zubair, Hira Bano, Riffat Ahmad, Shakil Shahab, Muhammad Reprod Med Biol Original Articles PROPOSE: The mechanism that underpins how RFRP‐3 and kisspeptin interacts are not fully understood in higher primates. This study therefore set out to assess RFRP‐3 and kisspeptin expression and their morphological interactions in the breeding, and in the non‐breeding period in monkey hypothalamus. METHODS: Eight mature male macaques (Macaca mulatta) in the breeding season (February; n = 4) and non‐breeding season (June; n = 4) were used. To reveal the expression and co‐localization of RFRP‐3 and kisspeptin, double‐labeled immunohistochemistry was performed. Testicular volume, sperm count, and plasma testosterone level were also measured to validate the breeding and non‐breeding paradigms. RESULTS: Testicular volume, plasma testosterone level, and sperm count showed a significant reduction during non‐breeding season. The number of kisspeptin‐positive cells was significantly increased during the breeding season (p < 0.05), whereas more RFRP‐3‐positive cell bodies were seen in the non‐breeding season (p < 0.01). Close contacts of RFRP‐3 fibers with kisspeptin cells showed no significant difference (p > 0.05) across seasons. However, co‐localization of RFRP‐3‐ir cell bodies onto kisspeptin IR cell bodies showed a statistical increase (p < 0.01) in non‐breeding season. CONCLUSION: In higher primates, RFRP‐3 decreases kisspeptin drives from the same cells to GnRH neurons in an autocrine manner causing suppression of the reproductive axis during the non‐breeding period. John Wiley and Sons Inc. 2022-07-08 /pmc/articles/PMC9270642/ /pubmed/35847413 http://dx.doi.org/10.1002/rmb2.12479 Text en © 2022 The Authors. Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Khan, Safdar
Batool, Bakhtwar
Zubair, Hira
Bano, Riffat
Ahmad, Shakil
Shahab, Muhammad
Expression and co‐localization of RFRP‐3 and kisspeptin during breeding and non‐breeding season in the hypothalamus of male rhesus monkey (Macaca mulatta)
title Expression and co‐localization of RFRP‐3 and kisspeptin during breeding and non‐breeding season in the hypothalamus of male rhesus monkey (Macaca mulatta)
title_full Expression and co‐localization of RFRP‐3 and kisspeptin during breeding and non‐breeding season in the hypothalamus of male rhesus monkey (Macaca mulatta)
title_fullStr Expression and co‐localization of RFRP‐3 and kisspeptin during breeding and non‐breeding season in the hypothalamus of male rhesus monkey (Macaca mulatta)
title_full_unstemmed Expression and co‐localization of RFRP‐3 and kisspeptin during breeding and non‐breeding season in the hypothalamus of male rhesus monkey (Macaca mulatta)
title_short Expression and co‐localization of RFRP‐3 and kisspeptin during breeding and non‐breeding season in the hypothalamus of male rhesus monkey (Macaca mulatta)
title_sort expression and co‐localization of rfrp‐3 and kisspeptin during breeding and non‐breeding season in the hypothalamus of male rhesus monkey (macaca mulatta)
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270642/
https://www.ncbi.nlm.nih.gov/pubmed/35847413
http://dx.doi.org/10.1002/rmb2.12479
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