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Detection of Mycoplasma gallisepticum in broiler chickens by PCR

BACKGROUND: Mycoplasma is a significant microorganism of poultry, which can cause respiratory infections and synovial inflammation, bringing about huge financial misfortunes to poultry workmanship worldwide. AIM: The goal of existing research was to determine the infection rate of Mycoplasma gallise...

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Autores principales: Mahmmoud, Ebtehal N., Hamad, Mohammad A., Khudhur, Zahraa N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Faculty of Veterinary Medicine 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270947/
https://www.ncbi.nlm.nih.gov/pubmed/35821769
http://dx.doi.org/10.5455/OVJ.2022.v12.i3.4
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author Mahmmoud, Ebtehal N.
Hamad, Mohammad A.
Khudhur, Zahraa N.
author_facet Mahmmoud, Ebtehal N.
Hamad, Mohammad A.
Khudhur, Zahraa N.
author_sort Mahmmoud, Ebtehal N.
collection PubMed
description BACKGROUND: Mycoplasma is a significant microorganism of poultry, which can cause respiratory infections and synovial inflammation, bringing about huge financial misfortunes to poultry workmanship worldwide. AIM: The goal of existing research was to determine the infection rate of Mycoplasma gallisepticum (MG) from chronic respiratory disease cases among broilers fields in Mosul/ Iraq using the polymerase chain reaction (PCR) technique. METHODS: All 92 lungs samples were collected from broilers with classical respiratory signs in different regions of the Nineveh governorate for 3 months from February to April 2021. RESULTS: PCR tests were performed using two couple primers, one for the qualitative amplification of 16S rRNA genes (285 base pairs) in Mycoplasma spp. and the second couple for the detection of M. gallisepticum (580 base pairs). Among the samples obtained from broilers, 87 (94.7%) were positive for Mycoplasma and 79 (85.9%) were positive for M. gallisepticum. CONCLUSION: Our results showed that MG infection in broiler chickens leads to serious clinical symptoms and severe lesions. The rate of Mycoplasma isolation in this study is high despite the short lifespan of broiler chickens.
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spelling pubmed-92709472022-07-11 Detection of Mycoplasma gallisepticum in broiler chickens by PCR Mahmmoud, Ebtehal N. Hamad, Mohammad A. Khudhur, Zahraa N. Open Vet J Original Research BACKGROUND: Mycoplasma is a significant microorganism of poultry, which can cause respiratory infections and synovial inflammation, bringing about huge financial misfortunes to poultry workmanship worldwide. AIM: The goal of existing research was to determine the infection rate of Mycoplasma gallisepticum (MG) from chronic respiratory disease cases among broilers fields in Mosul/ Iraq using the polymerase chain reaction (PCR) technique. METHODS: All 92 lungs samples were collected from broilers with classical respiratory signs in different regions of the Nineveh governorate for 3 months from February to April 2021. RESULTS: PCR tests were performed using two couple primers, one for the qualitative amplification of 16S rRNA genes (285 base pairs) in Mycoplasma spp. and the second couple for the detection of M. gallisepticum (580 base pairs). Among the samples obtained from broilers, 87 (94.7%) were positive for Mycoplasma and 79 (85.9%) were positive for M. gallisepticum. CONCLUSION: Our results showed that MG infection in broiler chickens leads to serious clinical symptoms and severe lesions. The rate of Mycoplasma isolation in this study is high despite the short lifespan of broiler chickens. Faculty of Veterinary Medicine 2022 2022-05-21 /pmc/articles/PMC9270947/ /pubmed/35821769 http://dx.doi.org/10.5455/OVJ.2022.v12.i3.4 Text en https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Mahmmoud, Ebtehal N.
Hamad, Mohammad A.
Khudhur, Zahraa N.
Detection of Mycoplasma gallisepticum in broiler chickens by PCR
title Detection of Mycoplasma gallisepticum in broiler chickens by PCR
title_full Detection of Mycoplasma gallisepticum in broiler chickens by PCR
title_fullStr Detection of Mycoplasma gallisepticum in broiler chickens by PCR
title_full_unstemmed Detection of Mycoplasma gallisepticum in broiler chickens by PCR
title_short Detection of Mycoplasma gallisepticum in broiler chickens by PCR
title_sort detection of mycoplasma gallisepticum in broiler chickens by pcr
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270947/
https://www.ncbi.nlm.nih.gov/pubmed/35821769
http://dx.doi.org/10.5455/OVJ.2022.v12.i3.4
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