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Distinct gene expression dynamics in developing and regenerating crustacean limbs
Regenerating animals have the ability to reproduce body parts that were originally made in the embryo and subsequently lost due to injury. Understanding whether regeneration mirrors development is an open question in most regenerative species. Here, we take a transcriptomics approach to examine whet...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9271199/ https://www.ncbi.nlm.nih.gov/pubmed/35776546 http://dx.doi.org/10.1073/pnas.2119297119 |
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author | Sinigaglia, Chiara Almazán, Alba Lebel, Marie Sémon, Marie Gillet, Benjamin Hughes, Sandrine Edsinger, Eric Averof, Michalis Paris, Mathilde |
author_facet | Sinigaglia, Chiara Almazán, Alba Lebel, Marie Sémon, Marie Gillet, Benjamin Hughes, Sandrine Edsinger, Eric Averof, Michalis Paris, Mathilde |
author_sort | Sinigaglia, Chiara |
collection | PubMed |
description | Regenerating animals have the ability to reproduce body parts that were originally made in the embryo and subsequently lost due to injury. Understanding whether regeneration mirrors development is an open question in most regenerative species. Here, we take a transcriptomics approach to examine whether leg regeneration shows similar temporal patterns of gene expression as leg development in the embryo, in the crustacean Parhyale hawaiensis. We find that leg development in the embryo shows stereotypic temporal patterns of gene expression. In contrast, the dynamics of gene expression during leg regeneration show a higher degree of variation related to the physiology of individual animals. A major driver of this variation is the molting cycle. We dissect the transcriptional signals of individual physiology and regeneration to obtain clearer temporal signals marking distinct phases of leg regeneration. Comparing the transcriptional dynamics of development and regeneration we find that, although the two processes use similar sets of genes, the temporal patterns in which these genes are deployed are different and cannot be systematically aligned. |
format | Online Article Text |
id | pubmed-9271199 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-92711992023-01-01 Distinct gene expression dynamics in developing and regenerating crustacean limbs Sinigaglia, Chiara Almazán, Alba Lebel, Marie Sémon, Marie Gillet, Benjamin Hughes, Sandrine Edsinger, Eric Averof, Michalis Paris, Mathilde Proc Natl Acad Sci U S A Biological Sciences Regenerating animals have the ability to reproduce body parts that were originally made in the embryo and subsequently lost due to injury. Understanding whether regeneration mirrors development is an open question in most regenerative species. Here, we take a transcriptomics approach to examine whether leg regeneration shows similar temporal patterns of gene expression as leg development in the embryo, in the crustacean Parhyale hawaiensis. We find that leg development in the embryo shows stereotypic temporal patterns of gene expression. In contrast, the dynamics of gene expression during leg regeneration show a higher degree of variation related to the physiology of individual animals. A major driver of this variation is the molting cycle. We dissect the transcriptional signals of individual physiology and regeneration to obtain clearer temporal signals marking distinct phases of leg regeneration. Comparing the transcriptional dynamics of development and regeneration we find that, although the two processes use similar sets of genes, the temporal patterns in which these genes are deployed are different and cannot be systematically aligned. National Academy of Sciences 2022-07-01 2022-07-05 /pmc/articles/PMC9271199/ /pubmed/35776546 http://dx.doi.org/10.1073/pnas.2119297119 Text en Copyright © 2022 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Biological Sciences Sinigaglia, Chiara Almazán, Alba Lebel, Marie Sémon, Marie Gillet, Benjamin Hughes, Sandrine Edsinger, Eric Averof, Michalis Paris, Mathilde Distinct gene expression dynamics in developing and regenerating crustacean limbs |
title | Distinct gene expression dynamics in developing and regenerating crustacean limbs |
title_full | Distinct gene expression dynamics in developing and regenerating crustacean limbs |
title_fullStr | Distinct gene expression dynamics in developing and regenerating crustacean limbs |
title_full_unstemmed | Distinct gene expression dynamics in developing and regenerating crustacean limbs |
title_short | Distinct gene expression dynamics in developing and regenerating crustacean limbs |
title_sort | distinct gene expression dynamics in developing and regenerating crustacean limbs |
topic | Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9271199/ https://www.ncbi.nlm.nih.gov/pubmed/35776546 http://dx.doi.org/10.1073/pnas.2119297119 |
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