Cargando…

Genotype-Specific Recruitment of Rhizosphere Bacteria From Sandy Loam Soil for Growth Promotion of Cucumis sativus var. hardwickii

The composition and structure of the rhizosphere microbiome is affected by many factors, including soil type, genotype, and cultivation time of the plant. However, the interaction mechanisms among these factors are largely unclear. We use culture-independent 16S rRNA amplicon sequencing to investiga...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Zeyang, Zheng, Yingying, Li, Yansu, Cheng, Xu, Huang, Sanwen, Yang, Xueyong, Qin, Yuxuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9271904/
https://www.ncbi.nlm.nih.gov/pubmed/35832804
http://dx.doi.org/10.3389/fmicb.2022.910644
_version_ 1784744776286863360
author Li, Zeyang
Zheng, Yingying
Li, Yansu
Cheng, Xu
Huang, Sanwen
Yang, Xueyong
Qin, Yuxuan
author_facet Li, Zeyang
Zheng, Yingying
Li, Yansu
Cheng, Xu
Huang, Sanwen
Yang, Xueyong
Qin, Yuxuan
author_sort Li, Zeyang
collection PubMed
description The composition and structure of the rhizosphere microbiome is affected by many factors, including soil type, genotype, and cultivation time of the plant. However, the interaction mechanisms among these factors are largely unclear. We use culture-independent 16S rRNA amplicon sequencing to investigate the rhizosphere bacterial composition and the structure of cultivated cucumber Xintaimici (XT) and wild-type cucumber Cucumis sativus var. hardwickii (HD) in four kinds of soils. We found that soil type, cultivation time, and genotype affected the composition and structure of cucumber rhizosphere bacterial communities. Notably, HD showed better physiological features in sandy soil and sandy loam soil than it did in black soil and farm soil at 50 days post-sowing, which was due to its stronger recruitment ability to Nitrospira, Nocardioides, Bacillus, and Gaiella in sandy soil, and more Tumebacillus, Nitrospira, and Paenibacillus in sandy loam soil. Meanwhile, we also found that HD showed a better recruiting capacity for these bacterial genera than XT in both sandy soil and sandy loam soil. Functional predictions indicated that these bacteria might have had stronger root colonization ability and then promoted the growth of cucumbers by enhancing nitrogen metabolism and active metabolite secretion. In this study, our findings provided a better insight into the relationship between cucumber phenotype, genotype, and the rhizosphere bacterial community, which will offer valuable theoretical references for rhizosphere microbiota studies and its future application in agriculture.
format Online
Article
Text
id pubmed-9271904
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-92719042022-07-12 Genotype-Specific Recruitment of Rhizosphere Bacteria From Sandy Loam Soil for Growth Promotion of Cucumis sativus var. hardwickii Li, Zeyang Zheng, Yingying Li, Yansu Cheng, Xu Huang, Sanwen Yang, Xueyong Qin, Yuxuan Front Microbiol Microbiology The composition and structure of the rhizosphere microbiome is affected by many factors, including soil type, genotype, and cultivation time of the plant. However, the interaction mechanisms among these factors are largely unclear. We use culture-independent 16S rRNA amplicon sequencing to investigate the rhizosphere bacterial composition and the structure of cultivated cucumber Xintaimici (XT) and wild-type cucumber Cucumis sativus var. hardwickii (HD) in four kinds of soils. We found that soil type, cultivation time, and genotype affected the composition and structure of cucumber rhizosphere bacterial communities. Notably, HD showed better physiological features in sandy soil and sandy loam soil than it did in black soil and farm soil at 50 days post-sowing, which was due to its stronger recruitment ability to Nitrospira, Nocardioides, Bacillus, and Gaiella in sandy soil, and more Tumebacillus, Nitrospira, and Paenibacillus in sandy loam soil. Meanwhile, we also found that HD showed a better recruiting capacity for these bacterial genera than XT in both sandy soil and sandy loam soil. Functional predictions indicated that these bacteria might have had stronger root colonization ability and then promoted the growth of cucumbers by enhancing nitrogen metabolism and active metabolite secretion. In this study, our findings provided a better insight into the relationship between cucumber phenotype, genotype, and the rhizosphere bacterial community, which will offer valuable theoretical references for rhizosphere microbiota studies and its future application in agriculture. Frontiers Media S.A. 2022-06-27 /pmc/articles/PMC9271904/ /pubmed/35832804 http://dx.doi.org/10.3389/fmicb.2022.910644 Text en Copyright © 2022 Li, Zheng, Li, Cheng, Huang, Yang and Qin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Li, Zeyang
Zheng, Yingying
Li, Yansu
Cheng, Xu
Huang, Sanwen
Yang, Xueyong
Qin, Yuxuan
Genotype-Specific Recruitment of Rhizosphere Bacteria From Sandy Loam Soil for Growth Promotion of Cucumis sativus var. hardwickii
title Genotype-Specific Recruitment of Rhizosphere Bacteria From Sandy Loam Soil for Growth Promotion of Cucumis sativus var. hardwickii
title_full Genotype-Specific Recruitment of Rhizosphere Bacteria From Sandy Loam Soil for Growth Promotion of Cucumis sativus var. hardwickii
title_fullStr Genotype-Specific Recruitment of Rhizosphere Bacteria From Sandy Loam Soil for Growth Promotion of Cucumis sativus var. hardwickii
title_full_unstemmed Genotype-Specific Recruitment of Rhizosphere Bacteria From Sandy Loam Soil for Growth Promotion of Cucumis sativus var. hardwickii
title_short Genotype-Specific Recruitment of Rhizosphere Bacteria From Sandy Loam Soil for Growth Promotion of Cucumis sativus var. hardwickii
title_sort genotype-specific recruitment of rhizosphere bacteria from sandy loam soil for growth promotion of cucumis sativus var. hardwickii
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9271904/
https://www.ncbi.nlm.nih.gov/pubmed/35832804
http://dx.doi.org/10.3389/fmicb.2022.910644
work_keys_str_mv AT lizeyang genotypespecificrecruitmentofrhizospherebacteriafromsandyloamsoilforgrowthpromotionofcucumissativusvarhardwickii
AT zhengyingying genotypespecificrecruitmentofrhizospherebacteriafromsandyloamsoilforgrowthpromotionofcucumissativusvarhardwickii
AT liyansu genotypespecificrecruitmentofrhizospherebacteriafromsandyloamsoilforgrowthpromotionofcucumissativusvarhardwickii
AT chengxu genotypespecificrecruitmentofrhizospherebacteriafromsandyloamsoilforgrowthpromotionofcucumissativusvarhardwickii
AT huangsanwen genotypespecificrecruitmentofrhizospherebacteriafromsandyloamsoilforgrowthpromotionofcucumissativusvarhardwickii
AT yangxueyong genotypespecificrecruitmentofrhizospherebacteriafromsandyloamsoilforgrowthpromotionofcucumissativusvarhardwickii
AT qinyuxuan genotypespecificrecruitmentofrhizospherebacteriafromsandyloamsoilforgrowthpromotionofcucumissativusvarhardwickii