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Dual-Action Icariin-Containing Thermosensitive Hydrogel for Wound Macrophage Polarization and Hair-Follicle Neogenesis

Bone morphogenetic protein (BMP) pathway is essential for M2 macrophage polarization and hair-follicle neogenesis. Icariin, a flavonoid derived from Epimedium, is a mediator of the BMP pathway. Here, we develop a hydrogel formulation functionalized with icariin for regulation of macrophage polarizat...

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Autores principales: Teng, Ying-Ying, Zou, Ming-Li, Liu, Si-Yu, Jia, Yuan, Zhang, Kai-Wen, Yuan, Zheng-Dong, Wu, Jun-Jie, Ye, Jun-Xing, Yu, Shun, Li, Xia, Zhou, Xiao-Jin, Yuan, Feng-Lai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9272914/
https://www.ncbi.nlm.nih.gov/pubmed/35832407
http://dx.doi.org/10.3389/fbioe.2022.902894
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author Teng, Ying-Ying
Zou, Ming-Li
Liu, Si-Yu
Jia, Yuan
Zhang, Kai-Wen
Yuan, Zheng-Dong
Wu, Jun-Jie
Ye, Jun-Xing
Yu, Shun
Li, Xia
Zhou, Xiao-Jin
Yuan, Feng-Lai
author_facet Teng, Ying-Ying
Zou, Ming-Li
Liu, Si-Yu
Jia, Yuan
Zhang, Kai-Wen
Yuan, Zheng-Dong
Wu, Jun-Jie
Ye, Jun-Xing
Yu, Shun
Li, Xia
Zhou, Xiao-Jin
Yuan, Feng-Lai
author_sort Teng, Ying-Ying
collection PubMed
description Bone morphogenetic protein (BMP) pathway is essential for M2 macrophage polarization and hair-follicle neogenesis. Icariin, a flavonoid derived from Epimedium, is a mediator of the BMP pathway. Here, we develop a hydrogel formulation functionalized with icariin for regulation of macrophage polarization to accelerate wound healing and hair-follicle neogenesis. Compared to skin defects without icariin treatment, those treated with icariin+PEG hydrogel healed faster and had new hair follicles. Results in vivo showed that icariin+PEG hydrogel induced a higher level of M2 phenotypic transformation of macrophages. Moreover, icariin+PEG hydrogel significantly accelerated wound-repair process by reducing the invasion of inflammation, excessive deposition of collagen, immoderate activation of myofibroblasts, and increasing the regeneration of hair follicles. Furthermore, studies in vitro demonstrated that the icariin+PEG hydrogel induced macrophages to polarize to the M2 phenotype and dermal papilla cell to hair follicles. Finally, molecular analysis demonstrated that the icariin+PEG hydrogel increased the expression of BMP4 and Smad1/5 phosphorylation in skin wounds. These results demonstrate the therapeutic potential of icariin-containing thermosensitive hydrogels for inducing M2 macrophage polarization to accelerate wound healing and promote hair-follicle neogenesis by regulating the BMP pathway.
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spelling pubmed-92729142022-07-12 Dual-Action Icariin-Containing Thermosensitive Hydrogel for Wound Macrophage Polarization and Hair-Follicle Neogenesis Teng, Ying-Ying Zou, Ming-Li Liu, Si-Yu Jia, Yuan Zhang, Kai-Wen Yuan, Zheng-Dong Wu, Jun-Jie Ye, Jun-Xing Yu, Shun Li, Xia Zhou, Xiao-Jin Yuan, Feng-Lai Front Bioeng Biotechnol Bioengineering and Biotechnology Bone morphogenetic protein (BMP) pathway is essential for M2 macrophage polarization and hair-follicle neogenesis. Icariin, a flavonoid derived from Epimedium, is a mediator of the BMP pathway. Here, we develop a hydrogel formulation functionalized with icariin for regulation of macrophage polarization to accelerate wound healing and hair-follicle neogenesis. Compared to skin defects without icariin treatment, those treated with icariin+PEG hydrogel healed faster and had new hair follicles. Results in vivo showed that icariin+PEG hydrogel induced a higher level of M2 phenotypic transformation of macrophages. Moreover, icariin+PEG hydrogel significantly accelerated wound-repair process by reducing the invasion of inflammation, excessive deposition of collagen, immoderate activation of myofibroblasts, and increasing the regeneration of hair follicles. Furthermore, studies in vitro demonstrated that the icariin+PEG hydrogel induced macrophages to polarize to the M2 phenotype and dermal papilla cell to hair follicles. Finally, molecular analysis demonstrated that the icariin+PEG hydrogel increased the expression of BMP4 and Smad1/5 phosphorylation in skin wounds. These results demonstrate the therapeutic potential of icariin-containing thermosensitive hydrogels for inducing M2 macrophage polarization to accelerate wound healing and promote hair-follicle neogenesis by regulating the BMP pathway. Frontiers Media S.A. 2022-06-27 /pmc/articles/PMC9272914/ /pubmed/35832407 http://dx.doi.org/10.3389/fbioe.2022.902894 Text en Copyright © 2022 Teng, Zou, Liu, Jia, Zhang, Yuan, Wu, Ye, Yu, Li, Zhou and Yuan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Teng, Ying-Ying
Zou, Ming-Li
Liu, Si-Yu
Jia, Yuan
Zhang, Kai-Wen
Yuan, Zheng-Dong
Wu, Jun-Jie
Ye, Jun-Xing
Yu, Shun
Li, Xia
Zhou, Xiao-Jin
Yuan, Feng-Lai
Dual-Action Icariin-Containing Thermosensitive Hydrogel for Wound Macrophage Polarization and Hair-Follicle Neogenesis
title Dual-Action Icariin-Containing Thermosensitive Hydrogel for Wound Macrophage Polarization and Hair-Follicle Neogenesis
title_full Dual-Action Icariin-Containing Thermosensitive Hydrogel for Wound Macrophage Polarization and Hair-Follicle Neogenesis
title_fullStr Dual-Action Icariin-Containing Thermosensitive Hydrogel for Wound Macrophage Polarization and Hair-Follicle Neogenesis
title_full_unstemmed Dual-Action Icariin-Containing Thermosensitive Hydrogel for Wound Macrophage Polarization and Hair-Follicle Neogenesis
title_short Dual-Action Icariin-Containing Thermosensitive Hydrogel for Wound Macrophage Polarization and Hair-Follicle Neogenesis
title_sort dual-action icariin-containing thermosensitive hydrogel for wound macrophage polarization and hair-follicle neogenesis
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9272914/
https://www.ncbi.nlm.nih.gov/pubmed/35832407
http://dx.doi.org/10.3389/fbioe.2022.902894
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