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Correlation of Nuclear Morphometry with Clinicopathologic Parameters in Malignant Breast Aspirates

Background  Nuclear size, shape, chromatin pattern, and nucleolar size and number have all been reported to change in breast cancer. Aim  The aim of the study was to quantify nuclear changes on malignant breast aspirates using morphometry and to correlate the morphometric parameters with clinicopath...

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Autores principales: Kalhan, Shivani, Garg, Shilpa, Satarkar, Rahul N., Sharma, Puja, Hasija, Sonia, Sharma, Sonia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Thieme Medical and Scientific Publishers Pvt. Ltd. 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273322/
https://www.ncbi.nlm.nih.gov/pubmed/35833049
http://dx.doi.org/10.1055/s-0040-1721215
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author Kalhan, Shivani
Garg, Shilpa
Satarkar, Rahul N.
Sharma, Puja
Hasija, Sonia
Sharma, Sonia
author_facet Kalhan, Shivani
Garg, Shilpa
Satarkar, Rahul N.
Sharma, Puja
Hasija, Sonia
Sharma, Sonia
author_sort Kalhan, Shivani
collection PubMed
description Background  Nuclear size, shape, chromatin pattern, and nucleolar size and number have all been reported to change in breast cancer. Aim  The aim of the study was to quantify nuclear changes on malignant breast aspirates using morphometry and to correlate the morphometric parameters with clinicopathologic features such as cytologic grade, tumor size, lymph node status, mitotic index, and histopathologic grade. Materials and Methods  Forty-five cases of carcinoma breast diagnosed on cytology were included in this study. Cytologic grading was performed as per the Robinson's cytologic grading system. Nuclear morphometry was done on Papanicolaou stained smears. One hundred nonoverlapping cells per case were evaluated. Both geometrical and textural parameters were evaluated. Results  Comparison of cytologic grades with most morphometric features (nuclear area, perimeter, shape, long axis, short axis, intensity, total run length, and TI homogeneity) was highly significant on statistical analysis. Correlation with tumor size yielded significant results for nuclear area, perimeter, long and short axes, and intensity with p  < 0.05. The study of lymph node status and morphometry showed a highly significant statistical association with all the parameters. Mitotic count was significantly associated with all the geometric parameters and one textural parameter (total run length). On correlation of ductal carcinoma in situ and histopathological Grades 1 to 3 with morphometry, it was found that all the parameters except long–run emphasis were highly significant with p  < 0.001. Conclusion  Morphometry as a technique holds immense promise in prognostication in breast carcinoma.
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spelling pubmed-92733222022-07-12 Correlation of Nuclear Morphometry with Clinicopathologic Parameters in Malignant Breast Aspirates Kalhan, Shivani Garg, Shilpa Satarkar, Rahul N. Sharma, Puja Hasija, Sonia Sharma, Sonia South Asian J Cancer Background  Nuclear size, shape, chromatin pattern, and nucleolar size and number have all been reported to change in breast cancer. Aim  The aim of the study was to quantify nuclear changes on malignant breast aspirates using morphometry and to correlate the morphometric parameters with clinicopathologic features such as cytologic grade, tumor size, lymph node status, mitotic index, and histopathologic grade. Materials and Methods  Forty-five cases of carcinoma breast diagnosed on cytology were included in this study. Cytologic grading was performed as per the Robinson's cytologic grading system. Nuclear morphometry was done on Papanicolaou stained smears. One hundred nonoverlapping cells per case were evaluated. Both geometrical and textural parameters were evaluated. Results  Comparison of cytologic grades with most morphometric features (nuclear area, perimeter, shape, long axis, short axis, intensity, total run length, and TI homogeneity) was highly significant on statistical analysis. Correlation with tumor size yielded significant results for nuclear area, perimeter, long and short axes, and intensity with p  < 0.05. The study of lymph node status and morphometry showed a highly significant statistical association with all the parameters. Mitotic count was significantly associated with all the geometric parameters and one textural parameter (total run length). On correlation of ductal carcinoma in situ and histopathological Grades 1 to 3 with morphometry, it was found that all the parameters except long–run emphasis were highly significant with p  < 0.001. Conclusion  Morphometry as a technique holds immense promise in prognostication in breast carcinoma. Thieme Medical and Scientific Publishers Pvt. Ltd. 2021-12-31 /pmc/articles/PMC9273322/ /pubmed/35833049 http://dx.doi.org/10.1055/s-0040-1721215 Text en MedIntel Services Pvt Ltd. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. ( https://creativecommons.org/licenses/by-nc-nd/4.0/ ) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited.
spellingShingle Kalhan, Shivani
Garg, Shilpa
Satarkar, Rahul N.
Sharma, Puja
Hasija, Sonia
Sharma, Sonia
Correlation of Nuclear Morphometry with Clinicopathologic Parameters in Malignant Breast Aspirates
title Correlation of Nuclear Morphometry with Clinicopathologic Parameters in Malignant Breast Aspirates
title_full Correlation of Nuclear Morphometry with Clinicopathologic Parameters in Malignant Breast Aspirates
title_fullStr Correlation of Nuclear Morphometry with Clinicopathologic Parameters in Malignant Breast Aspirates
title_full_unstemmed Correlation of Nuclear Morphometry with Clinicopathologic Parameters in Malignant Breast Aspirates
title_short Correlation of Nuclear Morphometry with Clinicopathologic Parameters in Malignant Breast Aspirates
title_sort correlation of nuclear morphometry with clinicopathologic parameters in malignant breast aspirates
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273322/
https://www.ncbi.nlm.nih.gov/pubmed/35833049
http://dx.doi.org/10.1055/s-0040-1721215
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