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Evaluation of Epidermal Growth Factor Receptor Gene Mutations in an Iranian Population with Non-Small Cell Lung Carcinoma
BACKGROUND: Mutations of the epidermal growth factor receptor (EGFR) gene, predominantly in exons 18–21, have been highlighted to function as the crucial predictors of the response rate of patients with non-small cell lung cancer (NSCLC) to EGFR tyrosine kinase inhibitors (TKIs). METHODS: This study...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tehran University of Medical Sciences
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273500/ https://www.ncbi.nlm.nih.gov/pubmed/35866114 http://dx.doi.org/10.18502/ijph.v51i2.8698 |
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author | Salmani, Hamzeh Yahyaei, Mahin Raoofian, Reza Irani, Shiva Salahshouri Far, Iman Akrami, Seyed Mohammad |
author_facet | Salmani, Hamzeh Yahyaei, Mahin Raoofian, Reza Irani, Shiva Salahshouri Far, Iman Akrami, Seyed Mohammad |
author_sort | Salmani, Hamzeh |
collection | PubMed |
description | BACKGROUND: Mutations of the epidermal growth factor receptor (EGFR) gene, predominantly in exons 18–21, have been highlighted to function as the crucial predictors of the response rate of patients with non-small cell lung cancer (NSCLC) to EGFR tyrosine kinase inhibitors (TKIs). METHODS: This study was performed at Tehran University of Medical Sciences. Data and information were retrospectively collected from the period between Dec 2010 and Apr 2014. Exons 18 to 21 of the EGFR were analyzed for any potential mutation by PCR, accompanied by DNA sequencing on 160 with pathological confirmation of NSCLC. RESULTS: Demographically, the male to female ratio was approximately 2:1, and a substantial difference in age between sexes was not observed (P=0.065), but a noticeable difference was found in the smoking variable, where 77.8% of males were smokers compared to 17.3% of women (odds ratio (OR) (95% CI) = 16.72 (7.15–39.11)). We found a frequency of 10.63% (17/160) for mutations found in exons 19 and 21, nonetheless, no mutations in exon 18 and exon 20 were observed. The most frequently observed mutations were c.2235_2249, del and c.2240_2257, del in exon 19 and p. L858R in exon 21. The c.2253A>G was found as a novel mutation that was the rarest mutation detected in this work. Interestingly, a remarkable negative association was revealed between smoking and mutation rates in NSCLC patients (OR (95% CI) = 0.13 (0.04–0.46). CONCLUSION: The occurrence of EGFR mutations is largely varied among the different states of Iran, probably due to variations in ethnicity, smoking rate, and sex ratio of participants. |
format | Online Article Text |
id | pubmed-9273500 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Tehran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-92735002022-07-20 Evaluation of Epidermal Growth Factor Receptor Gene Mutations in an Iranian Population with Non-Small Cell Lung Carcinoma Salmani, Hamzeh Yahyaei, Mahin Raoofian, Reza Irani, Shiva Salahshouri Far, Iman Akrami, Seyed Mohammad Iran J Public Health Original Article BACKGROUND: Mutations of the epidermal growth factor receptor (EGFR) gene, predominantly in exons 18–21, have been highlighted to function as the crucial predictors of the response rate of patients with non-small cell lung cancer (NSCLC) to EGFR tyrosine kinase inhibitors (TKIs). METHODS: This study was performed at Tehran University of Medical Sciences. Data and information were retrospectively collected from the period between Dec 2010 and Apr 2014. Exons 18 to 21 of the EGFR were analyzed for any potential mutation by PCR, accompanied by DNA sequencing on 160 with pathological confirmation of NSCLC. RESULTS: Demographically, the male to female ratio was approximately 2:1, and a substantial difference in age between sexes was not observed (P=0.065), but a noticeable difference was found in the smoking variable, where 77.8% of males were smokers compared to 17.3% of women (odds ratio (OR) (95% CI) = 16.72 (7.15–39.11)). We found a frequency of 10.63% (17/160) for mutations found in exons 19 and 21, nonetheless, no mutations in exon 18 and exon 20 were observed. The most frequently observed mutations were c.2235_2249, del and c.2240_2257, del in exon 19 and p. L858R in exon 21. The c.2253A>G was found as a novel mutation that was the rarest mutation detected in this work. Interestingly, a remarkable negative association was revealed between smoking and mutation rates in NSCLC patients (OR (95% CI) = 0.13 (0.04–0.46). CONCLUSION: The occurrence of EGFR mutations is largely varied among the different states of Iran, probably due to variations in ethnicity, smoking rate, and sex ratio of participants. Tehran University of Medical Sciences 2022-02 /pmc/articles/PMC9273500/ /pubmed/35866114 http://dx.doi.org/10.18502/ijph.v51i2.8698 Text en Copyright © 2022 Salmani et al. Published by Tehran University of Medical Sciences https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International license (https://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited. |
spellingShingle | Original Article Salmani, Hamzeh Yahyaei, Mahin Raoofian, Reza Irani, Shiva Salahshouri Far, Iman Akrami, Seyed Mohammad Evaluation of Epidermal Growth Factor Receptor Gene Mutations in an Iranian Population with Non-Small Cell Lung Carcinoma |
title | Evaluation of Epidermal Growth Factor Receptor Gene Mutations in an Iranian Population with Non-Small Cell Lung Carcinoma |
title_full | Evaluation of Epidermal Growth Factor Receptor Gene Mutations in an Iranian Population with Non-Small Cell Lung Carcinoma |
title_fullStr | Evaluation of Epidermal Growth Factor Receptor Gene Mutations in an Iranian Population with Non-Small Cell Lung Carcinoma |
title_full_unstemmed | Evaluation of Epidermal Growth Factor Receptor Gene Mutations in an Iranian Population with Non-Small Cell Lung Carcinoma |
title_short | Evaluation of Epidermal Growth Factor Receptor Gene Mutations in an Iranian Population with Non-Small Cell Lung Carcinoma |
title_sort | evaluation of epidermal growth factor receptor gene mutations in an iranian population with non-small cell lung carcinoma |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273500/ https://www.ncbi.nlm.nih.gov/pubmed/35866114 http://dx.doi.org/10.18502/ijph.v51i2.8698 |
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