Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning

Auxin critically regulates plant growth and development. Auxin-driven transcriptional responses are mediated through the AUXIN RESPONSE FACTOR (ARF) family of transcription factors. ARF protein condensation attenuates ARF activity, resulting in dramatic shifts in the auxin transcriptional landscape....

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Autores principales: Jing, Hongwei, Korasick, David A., Emenecker, Ryan J., Morffy, Nicholas, Wilkinson, Edward G., Powers, Samantha K., Strader, Lucia C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273615/
https://www.ncbi.nlm.nih.gov/pubmed/35817767
http://dx.doi.org/10.1038/s41467-022-31628-2
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author Jing, Hongwei
Korasick, David A.
Emenecker, Ryan J.
Morffy, Nicholas
Wilkinson, Edward G.
Powers, Samantha K.
Strader, Lucia C.
author_facet Jing, Hongwei
Korasick, David A.
Emenecker, Ryan J.
Morffy, Nicholas
Wilkinson, Edward G.
Powers, Samantha K.
Strader, Lucia C.
author_sort Jing, Hongwei
collection PubMed
description Auxin critically regulates plant growth and development. Auxin-driven transcriptional responses are mediated through the AUXIN RESPONSE FACTOR (ARF) family of transcription factors. ARF protein condensation attenuates ARF activity, resulting in dramatic shifts in the auxin transcriptional landscape. Here, we perform a forward genetics screen for ARF hypercondensation, identifying an F-box protein, which we named AUXIN RESPONSE FACTOR F-BOX1 (AFF1). Functional characterization of SCF(AFF1) revealed that this E3 ubiquitin ligase directly interacts with ARF19 and ARF7 to regulate their accumulation, condensation, and nucleo-cytoplasmic partitioning. Mutants defective in AFF1 display attenuated auxin responsiveness, and developmental defects, suggesting that SCF(AFF1) -mediated regulation of ARF protein drives aspects of auxin response and plant development.
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spelling pubmed-92736152022-07-13 Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning Jing, Hongwei Korasick, David A. Emenecker, Ryan J. Morffy, Nicholas Wilkinson, Edward G. Powers, Samantha K. Strader, Lucia C. Nat Commun Article Auxin critically regulates plant growth and development. Auxin-driven transcriptional responses are mediated through the AUXIN RESPONSE FACTOR (ARF) family of transcription factors. ARF protein condensation attenuates ARF activity, resulting in dramatic shifts in the auxin transcriptional landscape. Here, we perform a forward genetics screen for ARF hypercondensation, identifying an F-box protein, which we named AUXIN RESPONSE FACTOR F-BOX1 (AFF1). Functional characterization of SCF(AFF1) revealed that this E3 ubiquitin ligase directly interacts with ARF19 and ARF7 to regulate their accumulation, condensation, and nucleo-cytoplasmic partitioning. Mutants defective in AFF1 display attenuated auxin responsiveness, and developmental defects, suggesting that SCF(AFF1) -mediated regulation of ARF protein drives aspects of auxin response and plant development. Nature Publishing Group UK 2022-07-11 /pmc/articles/PMC9273615/ /pubmed/35817767 http://dx.doi.org/10.1038/s41467-022-31628-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Jing, Hongwei
Korasick, David A.
Emenecker, Ryan J.
Morffy, Nicholas
Wilkinson, Edward G.
Powers, Samantha K.
Strader, Lucia C.
Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning
title Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning
title_full Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning
title_fullStr Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning
title_full_unstemmed Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning
title_short Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning
title_sort regulation of auxin response factor condensation and nucleo-cytoplasmic partitioning
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273615/
https://www.ncbi.nlm.nih.gov/pubmed/35817767
http://dx.doi.org/10.1038/s41467-022-31628-2
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