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A Washing-Free and Easy-to-Operate Fluorescent Biosensor for Highly Efficient Detection of Breast Cancer-Derived Exosomes
Traditional detection methods for protein tumor markers in the early screening of breast cancer are restricted by complicated operation procedures and unstable reproducibility. As one of alternative emerging tumor markers, exosomes play an important role in diagnosing and treating cancers at the ear...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273779/ https://www.ncbi.nlm.nih.gov/pubmed/35837545 http://dx.doi.org/10.3389/fbioe.2022.945858 |
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author | Chen, Wenqin Zhang, Yan Di, Kaili Liu, Chang Xia, Yanyan Ding, Shijia Shen, Han Li, Zhiyang |
author_facet | Chen, Wenqin Zhang, Yan Di, Kaili Liu, Chang Xia, Yanyan Ding, Shijia Shen, Han Li, Zhiyang |
author_sort | Chen, Wenqin |
collection | PubMed |
description | Traditional detection methods for protein tumor markers in the early screening of breast cancer are restricted by complicated operation procedures and unstable reproducibility. As one of alternative emerging tumor markers, exosomes play an important role in diagnosing and treating cancers at the early stage due to traceability of their origins and great involvement in occurrence and development of cancers. Herein, a washing-free and efficient fluorescent biosensor has been proposed to realize simple and straightforward analysis of breast cancer cell-derived exosomes based on high affinity aptamers and G quadruplex-hemin (G4-hemin). The whole reaction process can be completed by several simple steps, which realizes washing-free and labor-saving. With simplified operation procedures and high repeatability, the linear detection range for this developed fluorescent biosensing strategy to breast cancer cell-derived exosomes is from 2.5 × 10(5) to 1.00 × 10(7) particles/ml, and the limit of detection is down to 0.54 × 10(5) particles/ml. |
format | Online Article Text |
id | pubmed-9273779 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92737792022-07-13 A Washing-Free and Easy-to-Operate Fluorescent Biosensor for Highly Efficient Detection of Breast Cancer-Derived Exosomes Chen, Wenqin Zhang, Yan Di, Kaili Liu, Chang Xia, Yanyan Ding, Shijia Shen, Han Li, Zhiyang Front Bioeng Biotechnol Bioengineering and Biotechnology Traditional detection methods for protein tumor markers in the early screening of breast cancer are restricted by complicated operation procedures and unstable reproducibility. As one of alternative emerging tumor markers, exosomes play an important role in diagnosing and treating cancers at the early stage due to traceability of their origins and great involvement in occurrence and development of cancers. Herein, a washing-free and efficient fluorescent biosensor has been proposed to realize simple and straightforward analysis of breast cancer cell-derived exosomes based on high affinity aptamers and G quadruplex-hemin (G4-hemin). The whole reaction process can be completed by several simple steps, which realizes washing-free and labor-saving. With simplified operation procedures and high repeatability, the linear detection range for this developed fluorescent biosensing strategy to breast cancer cell-derived exosomes is from 2.5 × 10(5) to 1.00 × 10(7) particles/ml, and the limit of detection is down to 0.54 × 10(5) particles/ml. Frontiers Media S.A. 2022-06-28 /pmc/articles/PMC9273779/ /pubmed/35837545 http://dx.doi.org/10.3389/fbioe.2022.945858 Text en Copyright © 2022 Chen, Zhang, Di, Liu, Xia, Ding, Shen and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Chen, Wenqin Zhang, Yan Di, Kaili Liu, Chang Xia, Yanyan Ding, Shijia Shen, Han Li, Zhiyang A Washing-Free and Easy-to-Operate Fluorescent Biosensor for Highly Efficient Detection of Breast Cancer-Derived Exosomes |
title | A Washing-Free and Easy-to-Operate Fluorescent Biosensor for Highly Efficient Detection of Breast Cancer-Derived Exosomes |
title_full | A Washing-Free and Easy-to-Operate Fluorescent Biosensor for Highly Efficient Detection of Breast Cancer-Derived Exosomes |
title_fullStr | A Washing-Free and Easy-to-Operate Fluorescent Biosensor for Highly Efficient Detection of Breast Cancer-Derived Exosomes |
title_full_unstemmed | A Washing-Free and Easy-to-Operate Fluorescent Biosensor for Highly Efficient Detection of Breast Cancer-Derived Exosomes |
title_short | A Washing-Free and Easy-to-Operate Fluorescent Biosensor for Highly Efficient Detection of Breast Cancer-Derived Exosomes |
title_sort | washing-free and easy-to-operate fluorescent biosensor for highly efficient detection of breast cancer-derived exosomes |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273779/ https://www.ncbi.nlm.nih.gov/pubmed/35837545 http://dx.doi.org/10.3389/fbioe.2022.945858 |
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