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A Washing-Free and Easy-to-Operate Fluorescent Biosensor for Highly Efficient Detection of Breast Cancer-Derived Exosomes

Traditional detection methods for protein tumor markers in the early screening of breast cancer are restricted by complicated operation procedures and unstable reproducibility. As one of alternative emerging tumor markers, exosomes play an important role in diagnosing and treating cancers at the ear...

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Detalles Bibliográficos
Autores principales: Chen, Wenqin, Zhang, Yan, Di, Kaili, Liu, Chang, Xia, Yanyan, Ding, Shijia, Shen, Han, Li, Zhiyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273779/
https://www.ncbi.nlm.nih.gov/pubmed/35837545
http://dx.doi.org/10.3389/fbioe.2022.945858
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author Chen, Wenqin
Zhang, Yan
Di, Kaili
Liu, Chang
Xia, Yanyan
Ding, Shijia
Shen, Han
Li, Zhiyang
author_facet Chen, Wenqin
Zhang, Yan
Di, Kaili
Liu, Chang
Xia, Yanyan
Ding, Shijia
Shen, Han
Li, Zhiyang
author_sort Chen, Wenqin
collection PubMed
description Traditional detection methods for protein tumor markers in the early screening of breast cancer are restricted by complicated operation procedures and unstable reproducibility. As one of alternative emerging tumor markers, exosomes play an important role in diagnosing and treating cancers at the early stage due to traceability of their origins and great involvement in occurrence and development of cancers. Herein, a washing-free and efficient fluorescent biosensor has been proposed to realize simple and straightforward analysis of breast cancer cell-derived exosomes based on high affinity aptamers and G quadruplex-hemin (G4-hemin). The whole reaction process can be completed by several simple steps, which realizes washing-free and labor-saving. With simplified operation procedures and high repeatability, the linear detection range for this developed fluorescent biosensing strategy to breast cancer cell-derived exosomes is from 2.5 × 10(5) to 1.00 × 10(7) particles/ml, and the limit of detection is down to 0.54 × 10(5) particles/ml.
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spelling pubmed-92737792022-07-13 A Washing-Free and Easy-to-Operate Fluorescent Biosensor for Highly Efficient Detection of Breast Cancer-Derived Exosomes Chen, Wenqin Zhang, Yan Di, Kaili Liu, Chang Xia, Yanyan Ding, Shijia Shen, Han Li, Zhiyang Front Bioeng Biotechnol Bioengineering and Biotechnology Traditional detection methods for protein tumor markers in the early screening of breast cancer are restricted by complicated operation procedures and unstable reproducibility. As one of alternative emerging tumor markers, exosomes play an important role in diagnosing and treating cancers at the early stage due to traceability of their origins and great involvement in occurrence and development of cancers. Herein, a washing-free and efficient fluorescent biosensor has been proposed to realize simple and straightforward analysis of breast cancer cell-derived exosomes based on high affinity aptamers and G quadruplex-hemin (G4-hemin). The whole reaction process can be completed by several simple steps, which realizes washing-free and labor-saving. With simplified operation procedures and high repeatability, the linear detection range for this developed fluorescent biosensing strategy to breast cancer cell-derived exosomes is from 2.5 × 10(5) to 1.00 × 10(7) particles/ml, and the limit of detection is down to 0.54 × 10(5) particles/ml. Frontiers Media S.A. 2022-06-28 /pmc/articles/PMC9273779/ /pubmed/35837545 http://dx.doi.org/10.3389/fbioe.2022.945858 Text en Copyright © 2022 Chen, Zhang, Di, Liu, Xia, Ding, Shen and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Chen, Wenqin
Zhang, Yan
Di, Kaili
Liu, Chang
Xia, Yanyan
Ding, Shijia
Shen, Han
Li, Zhiyang
A Washing-Free and Easy-to-Operate Fluorescent Biosensor for Highly Efficient Detection of Breast Cancer-Derived Exosomes
title A Washing-Free and Easy-to-Operate Fluorescent Biosensor for Highly Efficient Detection of Breast Cancer-Derived Exosomes
title_full A Washing-Free and Easy-to-Operate Fluorescent Biosensor for Highly Efficient Detection of Breast Cancer-Derived Exosomes
title_fullStr A Washing-Free and Easy-to-Operate Fluorescent Biosensor for Highly Efficient Detection of Breast Cancer-Derived Exosomes
title_full_unstemmed A Washing-Free and Easy-to-Operate Fluorescent Biosensor for Highly Efficient Detection of Breast Cancer-Derived Exosomes
title_short A Washing-Free and Easy-to-Operate Fluorescent Biosensor for Highly Efficient Detection of Breast Cancer-Derived Exosomes
title_sort washing-free and easy-to-operate fluorescent biosensor for highly efficient detection of breast cancer-derived exosomes
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273779/
https://www.ncbi.nlm.nih.gov/pubmed/35837545
http://dx.doi.org/10.3389/fbioe.2022.945858
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