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Golgi-Localized OsFPN1 is Involved in Co and Ni Transport and Their Detoxification in Rice
Cobalt (Co) and nickel (Ni) are beneficial and essential elements for plants, respectively, with the latter required for urease activity, which hydrolyzes urea into ammonium in plants. However, excess Co and Ni are toxic to plants and their transport mechanisms in rice are unclear. Here, we analyzed...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273799/ https://www.ncbi.nlm.nih.gov/pubmed/35817888 http://dx.doi.org/10.1186/s12284-022-00583-3 |
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author | Kan, Manman Fujiwara, Toru Kamiya, Takehiro |
author_facet | Kan, Manman Fujiwara, Toru Kamiya, Takehiro |
author_sort | Kan, Manman |
collection | PubMed |
description | Cobalt (Co) and nickel (Ni) are beneficial and essential elements for plants, respectively, with the latter required for urease activity, which hydrolyzes urea into ammonium in plants. However, excess Co and Ni are toxic to plants and their transport mechanisms in rice are unclear. Here, we analyzed an ethyl methanesulfonate (EMS)-mutagenized rice mutant, 1187_n, with increased Co and Ni contents in its brown rice and shoots. 1187_n has a mutation in OsFPN1, which was correlated with a high Co and Ni phenotype in F(2) crosses between the parental line and mutant. In addition, CRISPR/Cas9 mutants exhibited a phenotype similar to that of 1187_n, demonstrating that OsFPN1 is the causal gene of the mutant. In addition to the high Co and Ni in brown rice and shoots, the mutant also exhibited high Co and Ni concentrations in the xylem sap, but low concentrations in the roots, suggesting that OsFPN1 is involved in the root-to-shoot translocation of Co and Ni. The growth of 1187_n and CRISPR/Cas9 lines were suppressed under high Co and Ni condition, indicating OsFPN1 is required for the normal growth under high Co and Ni. An OsFPN1-green fluorescent protein (GFP) fusion protein was localized to the Golgi apparatus. Yeast carrying GFP-OsFPN1 increased sensitivity to high Co contents and decreased Co and Ni accumulation. These results suggest that OsFPN1 can transport Co and Ni and is vital detoxification in rice. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12284-022-00583-3. |
format | Online Article Text |
id | pubmed-9273799 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-92737992022-07-13 Golgi-Localized OsFPN1 is Involved in Co and Ni Transport and Their Detoxification in Rice Kan, Manman Fujiwara, Toru Kamiya, Takehiro Rice (N Y) Research Cobalt (Co) and nickel (Ni) are beneficial and essential elements for plants, respectively, with the latter required for urease activity, which hydrolyzes urea into ammonium in plants. However, excess Co and Ni are toxic to plants and their transport mechanisms in rice are unclear. Here, we analyzed an ethyl methanesulfonate (EMS)-mutagenized rice mutant, 1187_n, with increased Co and Ni contents in its brown rice and shoots. 1187_n has a mutation in OsFPN1, which was correlated with a high Co and Ni phenotype in F(2) crosses between the parental line and mutant. In addition, CRISPR/Cas9 mutants exhibited a phenotype similar to that of 1187_n, demonstrating that OsFPN1 is the causal gene of the mutant. In addition to the high Co and Ni in brown rice and shoots, the mutant also exhibited high Co and Ni concentrations in the xylem sap, but low concentrations in the roots, suggesting that OsFPN1 is involved in the root-to-shoot translocation of Co and Ni. The growth of 1187_n and CRISPR/Cas9 lines were suppressed under high Co and Ni condition, indicating OsFPN1 is required for the normal growth under high Co and Ni. An OsFPN1-green fluorescent protein (GFP) fusion protein was localized to the Golgi apparatus. Yeast carrying GFP-OsFPN1 increased sensitivity to high Co contents and decreased Co and Ni accumulation. These results suggest that OsFPN1 can transport Co and Ni and is vital detoxification in rice. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12284-022-00583-3. Springer US 2022-07-11 /pmc/articles/PMC9273799/ /pubmed/35817888 http://dx.doi.org/10.1186/s12284-022-00583-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Kan, Manman Fujiwara, Toru Kamiya, Takehiro Golgi-Localized OsFPN1 is Involved in Co and Ni Transport and Their Detoxification in Rice |
title | Golgi-Localized OsFPN1 is Involved in Co and Ni Transport and Their Detoxification in Rice |
title_full | Golgi-Localized OsFPN1 is Involved in Co and Ni Transport and Their Detoxification in Rice |
title_fullStr | Golgi-Localized OsFPN1 is Involved in Co and Ni Transport and Their Detoxification in Rice |
title_full_unstemmed | Golgi-Localized OsFPN1 is Involved in Co and Ni Transport and Their Detoxification in Rice |
title_short | Golgi-Localized OsFPN1 is Involved in Co and Ni Transport and Their Detoxification in Rice |
title_sort | golgi-localized osfpn1 is involved in co and ni transport and their detoxification in rice |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273799/ https://www.ncbi.nlm.nih.gov/pubmed/35817888 http://dx.doi.org/10.1186/s12284-022-00583-3 |
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