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Mambalgin-2 Inhibits Lung Adenocarcinoma Growth and Migration by Selective Interaction With ASIC1/α-ENaC/γ-ENaC Heterotrimer

Lung cancer is one of the most common cancer types in the world. Despite existing treatment strategies, overall patient survival remains low and new targeted therapies are required. Acidification of the tumor microenvironment drives the growth and metastasis of many cancers. Acid sensors such as aci...

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Autores principales: Sudarikova, Anastasia V., Bychkov, Maxim L., Kulbatskii, Dmitrii S., Chubinskiy-Nadezhdin, Vladislav I., Shlepova, Olga V., Shulepko, Mikhail A., Koshelev, Sergey G., Kirpichnikov, Mikhail P., Lyukmanova, Ekaterina N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273970/
https://www.ncbi.nlm.nih.gov/pubmed/35837090
http://dx.doi.org/10.3389/fonc.2022.904742
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author Sudarikova, Anastasia V.
Bychkov, Maxim L.
Kulbatskii, Dmitrii S.
Chubinskiy-Nadezhdin, Vladislav I.
Shlepova, Olga V.
Shulepko, Mikhail A.
Koshelev, Sergey G.
Kirpichnikov, Mikhail P.
Lyukmanova, Ekaterina N.
author_facet Sudarikova, Anastasia V.
Bychkov, Maxim L.
Kulbatskii, Dmitrii S.
Chubinskiy-Nadezhdin, Vladislav I.
Shlepova, Olga V.
Shulepko, Mikhail A.
Koshelev, Sergey G.
Kirpichnikov, Mikhail P.
Lyukmanova, Ekaterina N.
author_sort Sudarikova, Anastasia V.
collection PubMed
description Lung cancer is one of the most common cancer types in the world. Despite existing treatment strategies, overall patient survival remains low and new targeted therapies are required. Acidification of the tumor microenvironment drives the growth and metastasis of many cancers. Acid sensors such as acid-sensing ion channels (ASICs) may become promising targets for lung cancer therapy. Previously, we showed that inhibition of the ASIC1 channels by a recombinant analogue of mambalgin-2 from Dendroaspis polylepis controls oncogenic processes in leukemia, glioma, and melanoma cells. Here, we studied the effects and molecular targets of mambalgin-2 in lung adenocarcinoma A549 and Lewis cells, lung transformed WI-38 fibroblasts, and lung normal HLF fibroblasts. We found that mambalgin-2 inhibits the growth and migration of A549, metastatic Lewis P29 cells, and WI-38 cells, but not of normal fibroblasts. A549, Lewis, and WI-38 cells expressed different ASIC and ENaC subunits, while normal fibroblasts did not at all. Mambalgin-2 induced G2/M cell cycle arrest and apoptosis in lung adenocarcinoma cells. In line, acidification-evoked inward currents were observed only in A549 and WI-38 cells. Gene knockdown showed that the anti-proliferative and anti-migratory activity of mambalgin-2 is dependent on the expression of ASIC1a, α-ENaC, and γ-ENaC. Using affinity extraction and immunoprecipitation, mambalgin-2 targeting of ASIC1a/α-ENaC/γ-ENaC heteromeric channels in A549 cells was shown. Electrophysiology studies in Xenopus oocytes revealed that mambalgin-2 inhibits the ASIC1a/α-ENaC/γ-ENaC channels with higher efficacy than the ASIC1a channels, pointing on the heteromeric channels as a primary target of the toxin in cancer cells. Finally, bioinformatics analysis showed that the increased expression of ASIC1 and γ-ENaC correlates with a worse survival prognosis for patients with lung adenocarcinoma. Thus, the ASIC1a/α-ENaC/γ-ENaC heterotrimer can be considered a marker of cell oncogenicity and its targeting is promising for the design of new selective cancer therapeutics.
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spelling pubmed-92739702022-07-13 Mambalgin-2 Inhibits Lung Adenocarcinoma Growth and Migration by Selective Interaction With ASIC1/α-ENaC/γ-ENaC Heterotrimer Sudarikova, Anastasia V. Bychkov, Maxim L. Kulbatskii, Dmitrii S. Chubinskiy-Nadezhdin, Vladislav I. Shlepova, Olga V. Shulepko, Mikhail A. Koshelev, Sergey G. Kirpichnikov, Mikhail P. Lyukmanova, Ekaterina N. Front Oncol Oncology Lung cancer is one of the most common cancer types in the world. Despite existing treatment strategies, overall patient survival remains low and new targeted therapies are required. Acidification of the tumor microenvironment drives the growth and metastasis of many cancers. Acid sensors such as acid-sensing ion channels (ASICs) may become promising targets for lung cancer therapy. Previously, we showed that inhibition of the ASIC1 channels by a recombinant analogue of mambalgin-2 from Dendroaspis polylepis controls oncogenic processes in leukemia, glioma, and melanoma cells. Here, we studied the effects and molecular targets of mambalgin-2 in lung adenocarcinoma A549 and Lewis cells, lung transformed WI-38 fibroblasts, and lung normal HLF fibroblasts. We found that mambalgin-2 inhibits the growth and migration of A549, metastatic Lewis P29 cells, and WI-38 cells, but not of normal fibroblasts. A549, Lewis, and WI-38 cells expressed different ASIC and ENaC subunits, while normal fibroblasts did not at all. Mambalgin-2 induced G2/M cell cycle arrest and apoptosis in lung adenocarcinoma cells. In line, acidification-evoked inward currents were observed only in A549 and WI-38 cells. Gene knockdown showed that the anti-proliferative and anti-migratory activity of mambalgin-2 is dependent on the expression of ASIC1a, α-ENaC, and γ-ENaC. Using affinity extraction and immunoprecipitation, mambalgin-2 targeting of ASIC1a/α-ENaC/γ-ENaC heteromeric channels in A549 cells was shown. Electrophysiology studies in Xenopus oocytes revealed that mambalgin-2 inhibits the ASIC1a/α-ENaC/γ-ENaC channels with higher efficacy than the ASIC1a channels, pointing on the heteromeric channels as a primary target of the toxin in cancer cells. Finally, bioinformatics analysis showed that the increased expression of ASIC1 and γ-ENaC correlates with a worse survival prognosis for patients with lung adenocarcinoma. Thus, the ASIC1a/α-ENaC/γ-ENaC heterotrimer can be considered a marker of cell oncogenicity and its targeting is promising for the design of new selective cancer therapeutics. Frontiers Media S.A. 2022-06-28 /pmc/articles/PMC9273970/ /pubmed/35837090 http://dx.doi.org/10.3389/fonc.2022.904742 Text en Copyright © 2022 Sudarikova, Bychkov, Kulbatskii, Chubinskiy-Nadezhdin, Shlepova, Shulepko, Koshelev, Kirpichnikov and Lyukmanova https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Sudarikova, Anastasia V.
Bychkov, Maxim L.
Kulbatskii, Dmitrii S.
Chubinskiy-Nadezhdin, Vladislav I.
Shlepova, Olga V.
Shulepko, Mikhail A.
Koshelev, Sergey G.
Kirpichnikov, Mikhail P.
Lyukmanova, Ekaterina N.
Mambalgin-2 Inhibits Lung Adenocarcinoma Growth and Migration by Selective Interaction With ASIC1/α-ENaC/γ-ENaC Heterotrimer
title Mambalgin-2 Inhibits Lung Adenocarcinoma Growth and Migration by Selective Interaction With ASIC1/α-ENaC/γ-ENaC Heterotrimer
title_full Mambalgin-2 Inhibits Lung Adenocarcinoma Growth and Migration by Selective Interaction With ASIC1/α-ENaC/γ-ENaC Heterotrimer
title_fullStr Mambalgin-2 Inhibits Lung Adenocarcinoma Growth and Migration by Selective Interaction With ASIC1/α-ENaC/γ-ENaC Heterotrimer
title_full_unstemmed Mambalgin-2 Inhibits Lung Adenocarcinoma Growth and Migration by Selective Interaction With ASIC1/α-ENaC/γ-ENaC Heterotrimer
title_short Mambalgin-2 Inhibits Lung Adenocarcinoma Growth and Migration by Selective Interaction With ASIC1/α-ENaC/γ-ENaC Heterotrimer
title_sort mambalgin-2 inhibits lung adenocarcinoma growth and migration by selective interaction with asic1/α-enac/γ-enac heterotrimer
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273970/
https://www.ncbi.nlm.nih.gov/pubmed/35837090
http://dx.doi.org/10.3389/fonc.2022.904742
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