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mRNA expression and epigenetic-based role of chromodomain helicase DNA-binding 5 in hepatocellular carcinoma
OBJECTIVE: Chromodomain helicase DNA-binding 5 (CHD5) acts as a tumor suppressor gene in some cancers. CHD5 expression levels may affect an individual’s susceptibility to hepatocellular carcinoma (HCC). This study aimed to evaluate the methylation pattern of the CHD5 promoter region and the gene’s c...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9274423/ https://www.ncbi.nlm.nih.gov/pubmed/35808817 http://dx.doi.org/10.1177/03000605221105344 |
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author | Heidari, Zahra Asemi-Rad, Azam Moudi, Bita Mahmoudzadeh-Sagheb, Hamidreza |
author_facet | Heidari, Zahra Asemi-Rad, Azam Moudi, Bita Mahmoudzadeh-Sagheb, Hamidreza |
author_sort | Heidari, Zahra |
collection | PubMed |
description | OBJECTIVE: Chromodomain helicase DNA-binding 5 (CHD5) acts as a tumor suppressor gene in some cancers. CHD5 expression levels may affect an individual’s susceptibility to hepatocellular carcinoma (HCC). This study aimed to evaluate the methylation pattern of the CHD5 promoter region and the gene’s corresponding mRNA expression in HCC patients compared with healthy individuals. METHODS: In this case–control study, CHD5 mRNA gene expression levels and DNA methylation patterns were analyzed in 81 HCC patients and 90 healthy individuals by quantitative reverse transcription polymerase chain reaction and methylation-specific polymerase chain reaction, respectively. RESULTS: The CHD5 gene was hypermethylated in 61.8% of the HCC patients and 54.4% of the controls, and this difference was statistically significant. The CHD5 mRNA expression levels were significantly lower in the HCC patient group. CONCLUSIONS: Hypermethylation of the CHD5 promoter region may significantly lower the expression of this gene, affecting the incidence and severity of HCC. The methylation status of CHD5 can also be further studied as a prognostic factor in HCC. |
format | Online Article Text |
id | pubmed-9274423 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-92744232022-07-13 mRNA expression and epigenetic-based role of chromodomain helicase DNA-binding 5 in hepatocellular carcinoma Heidari, Zahra Asemi-Rad, Azam Moudi, Bita Mahmoudzadeh-Sagheb, Hamidreza J Int Med Res Prospective Clinical Research Report OBJECTIVE: Chromodomain helicase DNA-binding 5 (CHD5) acts as a tumor suppressor gene in some cancers. CHD5 expression levels may affect an individual’s susceptibility to hepatocellular carcinoma (HCC). This study aimed to evaluate the methylation pattern of the CHD5 promoter region and the gene’s corresponding mRNA expression in HCC patients compared with healthy individuals. METHODS: In this case–control study, CHD5 mRNA gene expression levels and DNA methylation patterns were analyzed in 81 HCC patients and 90 healthy individuals by quantitative reverse transcription polymerase chain reaction and methylation-specific polymerase chain reaction, respectively. RESULTS: The CHD5 gene was hypermethylated in 61.8% of the HCC patients and 54.4% of the controls, and this difference was statistically significant. The CHD5 mRNA expression levels were significantly lower in the HCC patient group. CONCLUSIONS: Hypermethylation of the CHD5 promoter region may significantly lower the expression of this gene, affecting the incidence and severity of HCC. The methylation status of CHD5 can also be further studied as a prognostic factor in HCC. SAGE Publications 2022-07-08 /pmc/articles/PMC9274423/ /pubmed/35808817 http://dx.doi.org/10.1177/03000605221105344 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by-nc/4.0/Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Prospective Clinical Research Report Heidari, Zahra Asemi-Rad, Azam Moudi, Bita Mahmoudzadeh-Sagheb, Hamidreza mRNA expression and epigenetic-based role of chromodomain helicase DNA-binding 5 in hepatocellular carcinoma |
title | mRNA expression and epigenetic-based role of chromodomain helicase
DNA-binding 5 in hepatocellular carcinoma |
title_full | mRNA expression and epigenetic-based role of chromodomain helicase
DNA-binding 5 in hepatocellular carcinoma |
title_fullStr | mRNA expression and epigenetic-based role of chromodomain helicase
DNA-binding 5 in hepatocellular carcinoma |
title_full_unstemmed | mRNA expression and epigenetic-based role of chromodomain helicase
DNA-binding 5 in hepatocellular carcinoma |
title_short | mRNA expression and epigenetic-based role of chromodomain helicase
DNA-binding 5 in hepatocellular carcinoma |
title_sort | mrna expression and epigenetic-based role of chromodomain helicase
dna-binding 5 in hepatocellular carcinoma |
topic | Prospective Clinical Research Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9274423/ https://www.ncbi.nlm.nih.gov/pubmed/35808817 http://dx.doi.org/10.1177/03000605221105344 |
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