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Transcription-associated metabolomic profiling reveals the critical role of frost tolerance in wheat
BACKGROUND: Low temperature is a crucial stress factor of wheat (Triticum aestivum L.) and adversely impacts on plant growth and grain yield. Multi-million tons of grain production are lost annually because crops lack the resistance to survive in winter. Particularlly, winter wheat yields was severe...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9275158/ https://www.ncbi.nlm.nih.gov/pubmed/35820806 http://dx.doi.org/10.1186/s12870-022-03718-2 |
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author | Lv, Liangjie Dong, Ce Liu, Yuping Zhao, Aiju Zhang, Yelun Li, Hui Chen, Xiyong |
author_facet | Lv, Liangjie Dong, Ce Liu, Yuping Zhao, Aiju Zhang, Yelun Li, Hui Chen, Xiyong |
author_sort | Lv, Liangjie |
collection | PubMed |
description | BACKGROUND: Low temperature is a crucial stress factor of wheat (Triticum aestivum L.) and adversely impacts on plant growth and grain yield. Multi-million tons of grain production are lost annually because crops lack the resistance to survive in winter. Particularlly, winter wheat yields was severely damaged under extreme cold conditions. However, studies about the transcriptional and metabolic mechanisms underlying cold stresses in wheat are limited so far. RESULTS: In this study, 14,466 differentially expressed genes (DEGs) were obtained between wild-type and cold-sensitive mutants, of which 5278 DEGs were acquired after cold treatment. 88 differential accumulated metabolites (DAMs) were detected, including P-coumaroyl putrescine of alkaloids, D-proline betaine of mino acids and derivativ, Chlorogenic acid of the Phenolic acids. The comprehensive analysis of metabolomics and transcriptome showed that the cold resistance of wheat was closely related to 13 metabolites and 14 key enzymes in the flavonol biosynthesis pathway. The 7 enhanced energy metabolites and 8 up-regulation key enzymes were also compactly involved in the sucrose and amino acid biosynthesis pathway. Moreover, quantitative real-time PCR (qRT-PCR) revealed that twelve key genes were differentially expressed under cold, indicating that candidate genes POD, Tacr7, UGTs, and GSTU6 which were related to cold resistance of wheat. CONCLUSIONS: In this study, we obtained the differentially expressed genes and differential accumulated metabolites in wheat under cold stress. Using the DEGs and DAMs, we plotted regulatory pathway maps of the flavonol biosynthesis pathway, sucrose and amino acid biosynthesis pathway related to cold resistance of wheat. It was found that candidate genes POD, Tacr7, UGTs and GSTU6 are related to cold resistance of wheat. This study provided valuable molecular information and new genetic engineering clues for the further study on plant resistance to cold stress. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-022-03718-2. |
format | Online Article Text |
id | pubmed-9275158 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-92751582022-07-13 Transcription-associated metabolomic profiling reveals the critical role of frost tolerance in wheat Lv, Liangjie Dong, Ce Liu, Yuping Zhao, Aiju Zhang, Yelun Li, Hui Chen, Xiyong BMC Plant Biol Research BACKGROUND: Low temperature is a crucial stress factor of wheat (Triticum aestivum L.) and adversely impacts on plant growth and grain yield. Multi-million tons of grain production are lost annually because crops lack the resistance to survive in winter. Particularlly, winter wheat yields was severely damaged under extreme cold conditions. However, studies about the transcriptional and metabolic mechanisms underlying cold stresses in wheat are limited so far. RESULTS: In this study, 14,466 differentially expressed genes (DEGs) were obtained between wild-type and cold-sensitive mutants, of which 5278 DEGs were acquired after cold treatment. 88 differential accumulated metabolites (DAMs) were detected, including P-coumaroyl putrescine of alkaloids, D-proline betaine of mino acids and derivativ, Chlorogenic acid of the Phenolic acids. The comprehensive analysis of metabolomics and transcriptome showed that the cold resistance of wheat was closely related to 13 metabolites and 14 key enzymes in the flavonol biosynthesis pathway. The 7 enhanced energy metabolites and 8 up-regulation key enzymes were also compactly involved in the sucrose and amino acid biosynthesis pathway. Moreover, quantitative real-time PCR (qRT-PCR) revealed that twelve key genes were differentially expressed under cold, indicating that candidate genes POD, Tacr7, UGTs, and GSTU6 which were related to cold resistance of wheat. CONCLUSIONS: In this study, we obtained the differentially expressed genes and differential accumulated metabolites in wheat under cold stress. Using the DEGs and DAMs, we plotted regulatory pathway maps of the flavonol biosynthesis pathway, sucrose and amino acid biosynthesis pathway related to cold resistance of wheat. It was found that candidate genes POD, Tacr7, UGTs and GSTU6 are related to cold resistance of wheat. This study provided valuable molecular information and new genetic engineering clues for the further study on plant resistance to cold stress. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-022-03718-2. BioMed Central 2022-07-11 /pmc/articles/PMC9275158/ /pubmed/35820806 http://dx.doi.org/10.1186/s12870-022-03718-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Lv, Liangjie Dong, Ce Liu, Yuping Zhao, Aiju Zhang, Yelun Li, Hui Chen, Xiyong Transcription-associated metabolomic profiling reveals the critical role of frost tolerance in wheat |
title | Transcription-associated metabolomic profiling reveals the critical role of frost tolerance in wheat |
title_full | Transcription-associated metabolomic profiling reveals the critical role of frost tolerance in wheat |
title_fullStr | Transcription-associated metabolomic profiling reveals the critical role of frost tolerance in wheat |
title_full_unstemmed | Transcription-associated metabolomic profiling reveals the critical role of frost tolerance in wheat |
title_short | Transcription-associated metabolomic profiling reveals the critical role of frost tolerance in wheat |
title_sort | transcription-associated metabolomic profiling reveals the critical role of frost tolerance in wheat |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9275158/ https://www.ncbi.nlm.nih.gov/pubmed/35820806 http://dx.doi.org/10.1186/s12870-022-03718-2 |
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